The Mesoamerican Ministers of Health have set 2020 as the target for malaria elimination to be achieved in the region. Imported malaria cases are a potential threat to countries attempting elimination or working to pr...The Mesoamerican Ministers of Health have set 2020 as the target for malaria elimination to be achieved in the region. Imported malaria cases are a potential threat to countries attempting elimination or working to prevent resurgence. We report the first imported Plasmodium ovale infection with molecular confirmation in Central America, which occurred in a Guatemalan soldier that had been deployed in Africa. The obstacles for its diagnosis using the standard microscopy technique and the need to improve its detection are discussed.展开更多
目的应用巢式PCR扩增小亚单位核糖体核糖核酸(18S r RNA)基因确诊卵形疟原虫感染。方法采集2012-2013年山东省疟疾患者全血和滤纸血样,吉氏染色镜检观察血膜疟原虫形态并鉴定虫种。提取血样中DNA,根据疟原虫18S r RNA基因以属和种特异...目的应用巢式PCR扩增小亚单位核糖体核糖核酸(18S r RNA)基因确诊卵形疟原虫感染。方法采集2012-2013年山东省疟疾患者全血和滤纸血样,吉氏染色镜检观察血膜疟原虫形态并鉴定虫种。提取血样中DNA,根据疟原虫18S r RNA基因以属和种特异性引物进行巢式PCR扩增,筛查卵形疟阳性标本并进行测序验证。结果2012和2013年山东省共筛查到7例输入性卵形疟原虫感染病例。巢式PCR扩增7份血样均在800 bp处出现卵形疟原虫特异性单一条带,Blast比对表明产物序列与Gen Bank卵形疟参考序列一致。结论经巢式PCR方法确诊了2012和2013年山东省7例输入性卵形疟病例。展开更多
文摘The Mesoamerican Ministers of Health have set 2020 as the target for malaria elimination to be achieved in the region. Imported malaria cases are a potential threat to countries attempting elimination or working to prevent resurgence. We report the first imported Plasmodium ovale infection with molecular confirmation in Central America, which occurred in a Guatemalan soldier that had been deployed in Africa. The obstacles for its diagnosis using the standard microscopy technique and the need to improve its detection are discussed.
文摘目的应用巢式PCR扩增小亚单位核糖体核糖核酸(18S r RNA)基因确诊卵形疟原虫感染。方法采集2012-2013年山东省疟疾患者全血和滤纸血样,吉氏染色镜检观察血膜疟原虫形态并鉴定虫种。提取血样中DNA,根据疟原虫18S r RNA基因以属和种特异性引物进行巢式PCR扩增,筛查卵形疟阳性标本并进行测序验证。结果2012和2013年山东省共筛查到7例输入性卵形疟原虫感染病例。巢式PCR扩增7份血样均在800 bp处出现卵形疟原虫特异性单一条带,Blast比对表明产物序列与Gen Bank卵形疟参考序列一致。结论经巢式PCR方法确诊了2012和2013年山东省7例输入性卵形疟病例。
