Expressions of HLA class Ⅰ and CD80 in human epithelial ovarian carcinomas

Objective:To determine expressions of HLA class I and CD80 in humanepithelial ovarian carcinomas(EOC) and the clinical significance.Methods:Expression of HLA class I was detected by immunohistochemical technique. Expression of CD80 mRNA was examined by reverse transcriptions-polymerase chain reaction(RT-PCR).Results:The positive rate of HLA classⅠ was 59.09%.CD80 mRNA was expressed on 9.09% of all 44 EOC tissues.HLA classⅠ expression rate in stage Ⅲ-Ⅳ was lower than that in stage Ⅰ-Ⅱ;in tumors of node-positive patients was lower than that of node-negative patients(P<0.05).In patiens with tumors expressing HLA class Ⅰ antigens,recurrence rate was lower than that in patients with tumors deficient in HLA class Ⅰ(P<0.01).In four patients with tumors expressing CD80 mRNA,recurrence did not occur,in contrast to patients with tumors lacking CD80 mRNA,in whom tumor relapse rate was 57.5%(P<0.05).Relapse ratein tumors deficient both HLA class Ⅰ and CD80 was significantly higher than that of tumors coexpression the two molecules.Conclusions:EOC cells may escapefrom the immune surveillance of the host through downregulating expressions ofHLA class Ⅰ and CD80.Evaluation of expressions of these surface immunoregulatory molecules may be helpful for judging prognoses of EOC patients and guiding immunotherapy.

卵巢上皮癌组织中HLA抗原和CD80的表达

目的 了解卵巢上皮癌 (EOC)中HLA、CD80的表达及其临床意义。方法 应用免疫组化法检测HLAⅠ和HLAⅡ的表达 ,用RT PCR检测CD80mRNA的表达。结果 44份EOC中 ,HLAⅠ、HLAⅡ的阳性表达率分别为 5 9 0 9%和 43 18% ;CD80mRNA的表达率为 9 0 9%。HLAⅠ类分子的阳性表达在Ⅲ～Ⅳ期显著低于Ⅰ～Ⅱ期 (P <0 0 5 ) ,有淋巴结转移组显著低于无淋巴结转移组 (P <0 0 5 ) ,肿瘤复发组显著低于未复发组 (P <0 0 1)。HLAⅡ类分子的表达在有淋巴结转移组显著低于无淋巴结转移组 (P <0 0 5 ) ,HLAⅡ表达组较不表达组复发率有降低趋势 ,但差异未达显著水平 (P =0 0 74)。CD80mRNA阳性者 4例均未复发 ,而CD80mRNA阴性者复发率为5 7 5 % ,两组有显著性差异 (P <0 0 5 )。HLAⅠ与HLAⅡ、HLAⅠ与CD80同时缺乏时 ,患者复发率分别较HLAⅠ与HLAⅡ、HLAⅠ与CD80共表达时显著升高 (P <0 0 1)。结论 EOC可能通过HLAⅠ的降调、CD80的缺乏多种途径逃避机体的免疫监视 ,检测上述调节分子的表达 ,可能对判断患者预后及指导免疫治疗有一定的意义。

免疫调节分子在卵巢上皮癌中的表达

目的 了解5种卵巢上皮癌(EOC)细胞系中免疫调节分子HLAⅠ、HLAⅡ、CD54、CD80)的表达,探讨EOC逃避机体免疫监视的机制。方法 采用流式细胞仪检测5种人EOC细胞系的HLAⅠ、HLAⅡ、CD54、CD80)表达;应用RT-PCR检测CD80mRNA的表达。结果 5种细胞系中仅COC1和COC2高表达HLAⅠ类分子(阳性细胞率在99％以上)。5种细胞系HLAⅡ类分子的表达均很低(1.40％～12.94％),SKOV3细胞CD54的表达较高(78.00％),COC1次之(33.25％),余者均较低。除COC2低表达CD80(6.63％)外,其余4种细胞系均不表达CD80。结论 5种EOC细胞系存在免疫调节分子表达的异常(如HLAⅠ下调、CD80的缺乏等),EOC细胞可能通过多个方面逃避机体的免疫监视。