Objective To investigate the correlation of interleukin（IL）-1,IL-6 and IL-10 concentrations to ovarian hyperstimulation syndrome（OHSS） and whether intravenous immunoglobulin（IVIG） has the effects on ovarian hyp...Objective To investigate the correlation of interleukin（IL）-1,IL-6 and IL-10 concentrations to ovarian hyperstimulation syndrome（OHSS） and whether intravenous immunoglobulin（IVIG） has the effects on ovarian hyperstimulated rats. Methods Immature female Wistar rats were divided into control group, OHSS group （n=13） and IVIG group（n=13）. For the latter two groups, pregnancy mare serum gonadotropin（PMSG）and human chorionic gonadotropin（hCG） were given to induce OHSS, and rats in IVIG group were treated with immunoglobulin. Forty-eight hours after administration of hCG, capillary permeability was evaluated from the Evans blue dye（EB） concentration in the ovaries and the EB concentration in peritoneal irrigated fluid at 30 min after the intravenous injection of EB. Rats＇ blood samples and ovaries were obtained to be measured for IL-1, IL-6 and IL-10 by ELISA. Results In OHSS group, total weights of bilateral ovaries and the ovarian EB concentration were significantly higher than those in others（P〈0.05）. Both serum and ovarian concentrations of IL-1 were significantly higher in OHSS and IVIG groups than those in control group （P〈0.05）. The ovarian concentrations of IL-6 and IL-10 in IVIG group were significantly lower than those in control group（P〈0.05）. Furthermore, the ovarian IL-10 concentration in IVIG group was significantly lower than that in OHSS group（P〈0. 05）. Conclusion Inflammation involved IL-1 in OHSS rats plays an important role. Vascular permeability was mostly increased in ovaries of hyperstimulated rats. It appears that ovaries of OHSS rats may be the primary places of inflammation. IVIG treatment resulted in statistically significant reductions in ovaries＇ weights and ovarian vascular permeability of OHSS rats, with a decreased level of ovarian IL-10. It implys that IVIG have a beneficial effect in reducing the severity of OHSS in the experimental model maybe by restrainning IL-10.展开更多
目的构建卵巢过度刺激综合征(OHSS)的大鼠模型。方法32只未成年Wistar雌鼠,随机分为常规刺激组和过度刺激组。过度刺激组:22日龄Wistar雌鼠连续4天皮下注射含有10 IU PMSG的生理盐水0.2ml,至26日龄皮下注射含有30 IU hCG的生理盐水0.2m...目的构建卵巢过度刺激综合征(OHSS)的大鼠模型。方法32只未成年Wistar雌鼠,随机分为常规刺激组和过度刺激组。过度刺激组:22日龄Wistar雌鼠连续4天皮下注射含有10 IU PMSG的生理盐水0.2ml,至26日龄皮下注射含有30 IU hCG的生理盐水0.2ml。设立对照组为常规刺激组:24日龄Wistar雌鼠皮下注射含有10IU PMSG的生理盐水0.2ml,再予皮下注射含有10IU hCG的生理盐水0.2ml。测血E2水平,尾静脉注入美兰溶液30min后取腹腔灌洗液和卵巢,用分光光度计分别测定腹腔灌洗液和卵巢组织中的美兰含量来判断腹膜和卵巢血管通透性;比较两组间卵巢重量,血E2水平及卵巢、腹腔液中美兰含量。结果注射hCG 48h后,过度刺激组大鼠卵巢重量、血E2水平、腹腔液和卵巢中美兰含量均显著高于常规刺激组。结论利用适当日龄Wister大鼠可以成功构建OHSS动物模型。展开更多
基金Nangjing Medicine Science and Technology Development Fund(No:YKK06100)
文摘Objective To investigate the correlation of interleukin（IL）-1,IL-6 and IL-10 concentrations to ovarian hyperstimulation syndrome（OHSS） and whether intravenous immunoglobulin（IVIG） has the effects on ovarian hyperstimulated rats. Methods Immature female Wistar rats were divided into control group, OHSS group （n=13） and IVIG group（n=13）. For the latter two groups, pregnancy mare serum gonadotropin（PMSG）and human chorionic gonadotropin（hCG） were given to induce OHSS, and rats in IVIG group were treated with immunoglobulin. Forty-eight hours after administration of hCG, capillary permeability was evaluated from the Evans blue dye（EB） concentration in the ovaries and the EB concentration in peritoneal irrigated fluid at 30 min after the intravenous injection of EB. Rats＇ blood samples and ovaries were obtained to be measured for IL-1, IL-6 and IL-10 by ELISA. Results In OHSS group, total weights of bilateral ovaries and the ovarian EB concentration were significantly higher than those in others（P〈0.05）. Both serum and ovarian concentrations of IL-1 were significantly higher in OHSS and IVIG groups than those in control group （P〈0.05）. The ovarian concentrations of IL-6 and IL-10 in IVIG group were significantly lower than those in control group（P〈0.05）. Furthermore, the ovarian IL-10 concentration in IVIG group was significantly lower than that in OHSS group（P〈0. 05）. Conclusion Inflammation involved IL-1 in OHSS rats plays an important role. Vascular permeability was mostly increased in ovaries of hyperstimulated rats. It appears that ovaries of OHSS rats may be the primary places of inflammation. IVIG treatment resulted in statistically significant reductions in ovaries＇ weights and ovarian vascular permeability of OHSS rats, with a decreased level of ovarian IL-10. It implys that IVIG have a beneficial effect in reducing the severity of OHSS in the experimental model maybe by restrainning IL-10.
文摘目的构建卵巢过度刺激综合征(OHSS)的大鼠模型。方法32只未成年Wistar雌鼠,随机分为常规刺激组和过度刺激组。过度刺激组:22日龄Wistar雌鼠连续4天皮下注射含有10 IU PMSG的生理盐水0.2ml,至26日龄皮下注射含有30 IU hCG的生理盐水0.2ml。设立对照组为常规刺激组:24日龄Wistar雌鼠皮下注射含有10IU PMSG的生理盐水0.2ml,再予皮下注射含有10IU hCG的生理盐水0.2ml。测血E2水平,尾静脉注入美兰溶液30min后取腹腔灌洗液和卵巢,用分光光度计分别测定腹腔灌洗液和卵巢组织中的美兰含量来判断腹膜和卵巢血管通透性;比较两组间卵巢重量,血E2水平及卵巢、腹腔液中美兰含量。结果注射hCG 48h后,过度刺激组大鼠卵巢重量、血E2水平、腹腔液和卵巢中美兰含量均显著高于常规刺激组。结论利用适当日龄Wister大鼠可以成功构建OHSS动物模型。
