期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Late-stage cascade of oxidation reactions during the biosynthesis of oxalicine B in Penicillium oxalicum
1
作者 Tao Zhang Guowei Gu +8 位作者 Guodong Liu Jinhua Su Zhilai Zhan Jianyuan Zhao Jinxiu Qian Guowei Cai Shan Cen Dewu Zhang Liyan Yu 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2023年第1期256-270,共15页
Oxalicine B(1)is an a-pyrone meroterpenoid with a unique bispirocyclic ring system derived from Penicillium oxalicum.The biosynthetic pathway of 15-deoxyoxalicine B(4)was preliminarily reported in Penicillium canescen... Oxalicine B(1)is an a-pyrone meroterpenoid with a unique bispirocyclic ring system derived from Penicillium oxalicum.The biosynthetic pathway of 15-deoxyoxalicine B(4)was preliminarily reported in Penicillium canescens,however,the genetic base and biochemical characterization of tailoring reactions for oxalicine B(1)has remained enigmatic.In this study,we characterized three oxygenases from the metabolic pathway of oxalicine B(1),including a cytochrome P450 hydroxylase OxaL,a hydroxylating Fe(II)/a-KG-dependent dioxygenase OxaK,and a multifunctional cytochrome P450 OxaB.Intriguingly,OxaK can catalyze various multicyclic intermediates or shunt products of oxalicines with impressive substrate promiscuity.OxaB was further proven via biochemical assays to have the ability to convert 15-hydroxdecaturin A(3)to 1 with a spiro-lactone core skeleton through oxidative rearrangement.We also solved the mystery of OxaL that controls C-15 hydroxylation.Chemical investigation of the wild-type strain and deletants enabled us to identify 10 metabolites including three new compounds,and the isolated compounds displayed potent anti-influenza A virus bioactivities exhibiting IC50values in the range of 4.0-19.9μmol/L.Our studies have allowed us to propose a late-stage biosynthetic pathway for oxalicine B(1)and create downstream derivatizations of oxalicines by employing enzymatic strategies. 展开更多
关键词 oxalicine B Meroterpenoid BIOSYNTHESIS Cytochrome P450 Anti-IAV activity
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部