Preparation and Properties of Anti-oxidation Inorganic Nano-coating for Low Carbon Steel at an Elevated Temperature

A new kind of anti-oxidation inorganic nano-coating for the common low carbon steel was prepared. It included magnesite mineral, metallurgic dust and silicate adhesive as the main raw materials. The nano-coating could be sprayed directly onto the low carbon steel slab even though with hot surface as far as under 1000 ℃ . And at the same time, a compact thin nano-film was formed, and the film would inhibit the oxygen into the interface of the steel body and decrease the loss of weight because of oxidation. The loss was decreased by about 60% or more. The properties and mechanism of oxidation resistance of the coating were discussed through XRD, TG-DTA and SEM. The experimental results show that many reactions would happen among the companents of the coating and then many microspheres with the size of 80- 100nm generated in the system. By using the heat of the steel body, the silicate adhesive would interact with the microspheres, and the other components of the coating would be soft and sintered so that when the coating was sprayed onto the suface of the steel slab , the intact and compact film could be formed and adhesive with the steel body. Besides the special properties of anti- oxidation, the coating could prevent the volatilization of microelement in the steel such as silicate and carbon at a high temperature. So it can improve the quality and output of steel with this new kind of nano-coating.

Oxidative imbalance increases the risk for colonic polyp and colorectal cancer development

BACKGROUND The role of oxidative stress in the pathogenesis of colorectal carcinoma(CRC)has garnered considerable interest recently.Specific oxidative factors have been implicated in the pathogenesis of adenomatous polyps and ultimately adenocarcinoma.AIM To evaluate the effect of oxidative imbalance as quantified by specific serological markers in the development of sporadic colon adenocarcinoma.METHODS A total of 170 patients that underwent endoscopy of the lower gastrointestinal tract in a tertiary center within 3 years were included in the study.They were allocated in three groups;those with sporadic colon adenocarcinoma(n=56,32.9%),those with colonic polyps(n=33,19.4%)and healthy controls(n=81,47.7%).All patients were evaluated for oxidant activity and antioxidant capacity with serum measurements of specific markers such as vitamins A,25(OH)D3,E,C,B12,folic acid,glutathione,selenium(Se),zinc(Zn),free iron(Fe^(2+)),and malondialdehyde and results were compared between groups.RESULTS Serum levels of vitamins C,E,D,Se,Zn,vitamin B12 and total antioxidant capacity were significantly lower in the combined neoplasia/polyp group than in the control group(P=0.002,P=0.009,P<0.001,P<0.001,P<0.001,P=0.020 and P<0.001,correspondingly).Increased levels of vitamin E(P=0.004),vitamin D(P<0.001),Se(P<0.001)and Zn(P<0.001)seem to bestow a protective effect on the development of CRC.For vitamin D(P<0.001)and Zn(P=0.036),this effect seems to extend to the development of colon polyps as well.On the other hand,elevated serum levels of malondialdehyde are associated with a higher risk of CRC(OR=2.09 compared to controls,P=0.004).Regarding colonic polyp development,increased concentrations of vitaminΑand Fe^(2+) are associated with a higher risk,whereas lower levels of malondialdehyde with a lower risk.CONCLUSION Increased oxidative stress may play an important role in the pathogenesis and progression of CRC.Antioxidants’presence may exert a protective effect in the very early stages of colon carcinogenesis.

Etomidate affects the anti-oxidant pathway to protect retinal ganglion cells after optic nerve transection

Our previous studies revealed that etomidate, a non-barbiturate intravenous anesthetic agent, has protective effects on retinal ganglion cells within 7 days after optic nerve transection. Whether this process is related to anti-oxidative stress is not clear. To reveal its mechanism, we established the optic nerve transection injury model by transecting 1 mm behind the left eyeball of adult male Sprague-Dawley rats. The rats received an intraperitoneal injection of etomidate(4 mg/kg) once per day for 7 days. The results showed that etomidate significantly enhanced the number of retinal ganglion cells retrogradely labeled with Fluorogold at 7 days after optic nerve transection. Etomidate also significantly reduced the levels of nitric oxide and malonaldehyde in the retina and increased the level of glutathione at 12 hours after optic nerve transection. Thus, etomidate can protect retinal ganglion cells after optic nerve transection in adult rats by activating an anti-oxidative stress response. The study was approved by the Animal Ethics Committee at Air Force Medical University, China(approval No. 20180305) on March 5, 2018.

Redox imbalance stress in diabetes mellitus: Role of the polyol pathway

In diabetes mellitus, the polyol pathway is highly active and consumes approximately 30% glucose in the body. This pathway contains 2 reactions catalyzed by aldose reductase(AR) and sorbitol dehydrogenase, respectively. AR reduces glucose to sorbitol at the expense of NADPH, while sorbitol dehydrogenase converts sorbitol to fructose at the expense of NAD+, leading to NADH production. Consumption of NADPH, accumulation of sorbitol, and generation of fructose and NADH have all been implicated in the pathogenesis of diabetes and its complications. In this review, the roles of this pathway in NADH/NAD+redox imbalance stress and oxidative stress in diabetes are highlighted. A potential intervention using nicotinamide riboside to restore redox balance as an approach to fighting diabetes is also discussed.

Microstructure Study on Oxidation of Zirconium Diboride

The oxidation behaviors of fused zirconium diboride and chemosynthetic zirconium diboride as well as morphology and composition of their oxidation products were researched by FESEM-EDS and XRD.The two kinds of zirconium diboride were heated at 700℃,900℃,1100℃and 1300℃for 3 h in air,respectively.The results show that Zr02 and B203(Ⅰ)are generated from the chemosynthetic zirconium diboride oxidized at 700℃for 3 h or the fused zirconium diboride oxidized at 800℃for 24 h;B203(Ⅰ)dissolves into water and then H3B03 crystallizes.

Overall Estimation of Anti-Oxidant Activity by Mammal Macrophage

Super oxide anion had been known as a critical role in a phagocytic cell when microorganisms invade inside. However, this radical seldom need for a clean environment such in a developed country. Moreover, this agent sometimes triggered as serious factor in the site of inflammation. There are many papers which concerned newly found anti-oxidative substances. However, many reports only focused on the molecular basis in vitro, how oxidative stress damaged the human tissue. The key factor for oxidative stress needs to be discussed first for immunological standpoint, such as armed reduction treaty. In other words, minimum armed force is necessary for an invasion by outer or inner irregular cells. On the basis of phagocytic function of the cell in vivo, we tried to access anti-oxidative activity by employing peritoneal murine macrophage and human monocyte. So, the purpose of this study is to establish a conventional but tailor made system for which is useful for evaluating the anti-oxidative activity to different constition. Especially in human peripheral monocyte, the sample was tested each constitution such in granulocyte rich type and lymphocyte rich typ. In order to establish the direct estimation by phagocytic cell, a murine peritoneal exudates cell suspension was obtained after one week of oral administration with anti-oxidative sample. The samples we selected were traditional Chinese medicine, Shi-Quan-Da-Bu-Tang (SDT), Bu-Zong-Ye-Qi-Tang (BYT) and Shao-Chin-Rong-Tang sCRT), Agaricus, Chlorella, and Propolis. The amount of generated super oxide anion was calculated through measuring the optical density change of this supernatant using cytochrome-C which was reduced by generated super oxide anion in the phagocytic cell. The generated super oxide anion after administration of Agaricus, Chlorella, SDT, BYT, sCRT and Propolis were 2.64, 1.95, 1.24, 1.25, 2.88, 2.55 × 10﹣5 mmol/ml, respectively, whereas 2.85 × 10﹣5 mmol/ml in control group. The fermented sample from SDT, BYT, sCRT and microfied Propolis were also tested and the value of super oxide anion were 0.62, 0.84, 1.50 and 2.55 × 10﹣5 mmol/ml, respectively. In this report, we discussed the direct method for measuring the generated super oxide anion of macrophages, especially from different constitution.

加味升降散对早期糖尿病肾病患者肾小管功能的影响

目的:观察加味升降散对早期糖尿病肾病(DN)患者肾小管功能的影响及其作用机制。方法:选取2018年3月至2019年10月河北省中医院肾内科门诊及住院部收治的早期DN患者92例作为研究对象,中医辨证属气阴两虚、浊毒郁热证。用简单随机化法分为对照组和观察组,每组46例。对照组给予基础治疗;观察组在基础治疗上加服加味升降散,疗程均为2个月。记录2组患者治疗前后中医证候积分;临床指标:糖化血红蛋白(HbA1c),低密度脂蛋白(LDL),总胆固醇(TC),尿微量白蛋白排泄率(UAER),肾小球滤过率(GFR);肾小管功能标志物:β2-微球蛋白(β2-MG),肝型脂肪酸结合蛋白(L-FABP),α1-微球蛋白(α1-MG);氧化应激指标:NADPH氧化酶4(NOX4),超氧化物歧化酶(SOD),总抗氧化能力(T-AOC)。结果:治疗后,观察组总有效率为92.68%,对照组为81.40%,差异有统计学意义(P<0.05);2组患者中医证候积分、HbA1c、TC、LDL、UAER均较前下降,且观察组下降更为显著(均P<0.05);2组患者治疗前后GFR变化不明显,α1-MG、β2-MG、L-FABP水平均较前降低,且观察组下降更显著(均P<0.05);2组患者NOX4表达受到抑制,T-AOC、SOD水平回升(均P<0.05),观察组患者NOX4表达的下降以及T-AOC、SOD水平的回升更显著(P<0.05);2组患者均未出现不良反应。结论:加味升降散能够改善早期DN患者临床症状,调节氧化-抗氧化失衡,减轻氧化应激,缓解肾小管损伤。

圆锥角膜铁稳态失衡的研究进展

铁离子在维持角膜细胞正常代谢、DNA合成和修复等生理活动中发挥关键作用,但过量的铁离子可能引发铁稳态失衡继而导致细胞毒性损伤和死亡。圆锥角膜是最常见的扩张性角膜疾病,其典型的Fleischer环是铁稳态失衡的直接证据。圆锥角膜与铁代谢相关的前期研究显示,铁稳态失衡有可能是诱发圆锥角膜发生和发展的潜在致病机制。文章总结了人体及角膜中正常的铁代谢循环以及圆锥角膜铁稳态失衡的证据,并从维持铁稳态角度出发探索可能的治疗策略,为扩张性眼病治疗提供新的思路。

Unveiling the dynamic role of innate and adaptive immune cells in COPD pathogenesis induced by cigarette smoke

Chronic obstructive pulmonary disease(COPD)is a multifaceted syndrome characterized by a dysregulated inflammatory cascade within the respiratory system,primarily triggered by exposure to harmful particles and gases,notably from cigarette smoke.This inflammatory response is orchestrated by innate immune cells like macrophages and epithelial cells,which recognize danger signals released from damaged cells.Prolonged inflammation prompts an adaptive immune reaction mediated by dendritic cells,culminating in the formation of lymphoid follicles and involving a complex interplay of T and B cells,as well as cytotoxic activity.Additionally,both viral and bacterial infections exacerbate COPD by further igniting inflammatory pathways,perpetuating the chronic inflammatory state.This comprehensive review encapsulates the intricate interplay between innate and adaptive immunity in COPD,with a particular focus on the role of cigarette smoke in its pathogenesis and potential therapeutic targets.

Inflammation, oxidative stress and renin angiotensin system in atherosclerosis

Atherosclerosis is a chronic inflammatory disease associated with cardiovascular dysfunction including myocardial infarction, unstable angina, sudden cardiac death, stroke and peripheral thromboses. It has been predicted that atherosclerosis will be the primary cause of death in the world by 2020. Atherogenesis is initiated by endothelial injury due to oxidative stress associated with cardiovascular risk factors including diabetes mellitus, hypertension, cigarette smoking, dyslipidemia, obesity, and metabolic syndrome. The impairment of the endothelium associated with cardiovascular risk factors creates an imbalance between vasodilating and vasoconstricting factors, in particular, an increase in angiotensin Ⅱ(Ang Ⅱ) and a decrease in nitric oxide. The renin-angiotensin system(RAS), and its primary mediator Ang Ⅱ, also have a direct influence on the progression of the atherosclerotic process via effects on endothelial function, inflammation, fibrinolytic balance, and plaque stability. Anti-inflammatory agents [statins, secretory phospholipase A2 inhibitor, lipoprotein-associated phospholipase A2 inhibitor, 5-lipoxygenase activating protein, chemokine motif ligand-2, C-C chemokine motif receptor 2 pathway inhibitors, methotrexate, IL-1 pathway inhibitor and RAS inhibitors(angiotensin-converting enzyme inhibitors)], Ang Ⅱ receptor blockers and ranin inhibitors may slow inflammatory processes and disease progression. Several studies in human using anti-inflammatory agents and RAS inhibitors revealed vascular benefits and reduced progression of coronary atherosclerosis in patients with stable angina pectoris; decreased vascular inflammatory markers, improved common carotid intima-media thickness and plaque volume in patients with diagnosed atherosclerosis. Recent preclinical studies have demonstrated therapeutic efficacy of vitamin D analogs paricalcitol in Apo E-deficient atherosclerotic mice.

Modulation of Anti-Oxidation Ability by Proanthocyanidins during Germination of Arabidopsis thaliana Seeds

Proanthocyanidins （PAs） as the end products of flavonoid biosynthetic pathway mainly accumulate in seed coat but their biological function is largely unknown. We studied the anti-oxidation ability in seed coat and germination changes under externally applied oxidative stresses in PAs-deficient mutants of Arabidopsis. Germination of PAs-deficient mutant seeds was faster than that of wild-type under low or no oxidative stress, suggesting a PAs-induced inhibition of germination. When the applied oxidative stress was high, germination of PAs-deficient mutants was lower than that of wild-type, suggesting a loss of PAs-related anti-oxidation ability in the mutants. Using ABA signaling mutants, our studies demonstrated that both ABA signaling pathway and PAs were important for the response to serve oxidative stress during seed germination. However, the discrepancy of the response between abi mutants and PAs mutants to oxidative stress suggests that ABA signaling pathway may not play a major role in PAs＂ action in alleviating oxidative stress. Under low or no oxidative stress, germination was mainly determined by the ABA content in seed and the PAs-deficient mutant seeds germinated faster due to their lower ABA content than wild-type. However, oxidative injury inhibited germination when PAs-deficient seeds germinated under high oxidative stress, Wild-type exhibited higher germination under the high ox- idative stress due to the PAs＇ anti-oxidation ability. Oxidative stress applied externally led to changes in endogenous PAs contents that coincided with the expression changes of PAs biogenesis genes. PAs modulated the activities of some key enzymes that controlled the levels of reactive oxygen species and the anti-oxidation capacity during the seed germination. This work suggests that PAs contribute to the adaptive mechanism that helps germination under environmental stresses by playing dual roles in both germination control and anti-oxidation reaction.

Review of oxidative stress and antioxidative defense mechanisms in Gossypium hirsutum L.in response to extreme abiotic conditions

Oxidative stress occurs when crop plants are exposed to extreme abiotic conditions that lead to the excessive production and accumulation of reactive oxygen species(ROS).Those extreme abiotic conditions or stresses include drought,high temperature,heavy metals,salinity,and ultraviolet radiation,and they cause yield and quality losses in crops.ROS are highly reactive species found in nature that can attack plant organelles,metabolites,and molecules by interrupting various metabolic pathways until cell death occurs.Plants have evolved defense mechanisms for the production of antioxidants to detoxify the ROS and to protect the plant against oxidative damage.Modern researches in crop plants revealed that low levels of ROS act as a signal which induces tolerance to environmental extremes by altering the expression of defensive genes.In this review,we summarized the processes involved in ROS production in response to several types of abiotic stress in cotton plants.Furthermore,we discussed the achievements in the understanding and improving oxidative stress tolerance in cotton in recent years.Researches related to plant oxidative stresses have shown excellent potential for the development of stress-tolerant crops.

Oxidative stress in testicular tissues of rats exposed to cigarette smoke and protective effects of caffeic acid phenethyl ester

Aim： To show the oxidative stress after cigarette smoke exposure in rat testis and to evaluate the effects of caffeic acid phenethyl ester （CAPE）. Methods： Twenty-one rats were divided into three groups of seven. Animals in Group Ⅰ were used as control. Rats in Group Ⅱ were exposed to cigarette smoke only （4 × 30 min/d） and rats in Group Ⅲ were exposed to cigarette smoke and received daily intraperitoneal injections of CAPE （10 μmol/kg.d）. After 60 days all the rats were killed and the levels of nitric oxide （NO） and anti-oxidant enzymes such as superoxide-dismutase, catalase and glutathione peroxidase （GSH-Px） and the level of malondialdehyde were studied in the testicular tissues of rats with spectrophotometric analysis. Results： There was a significant increase in catalase and superoxide-dismutase activities in Group Ⅱ when compared to the controls, but the levels of both decreased after CAPE administration in Group Ⅲ. GSH-Px activity was decreased in Group Ⅱ but CAPE caused an elevation in GSH-Px activity in Group Ⅲ. The difference between the levels of GSH-Px in Group Ⅰ and Group Ⅱ was significant, but the difference between groups Ⅱ and Ⅲ was not significant. Elevation of malondialdehyde after smoke exposure was significant and CAPE caused a decrease to a level which was not statistically different to the control group. A significantly increased level of NO after exposure to smoke was reversed by CAPE administration and the difference between NO levels in groups Ⅰ and Ⅲ was statistically insignificant. Conclusion： Exposure to cigarette smoke causes changes in the oxidative enzyme levels in rat testis, but CAPE can reverse these harmful effects. （Asian J Andro12006 Mar; 8： 189-193）

Impact of obesity and Ala16Val MnSOD polymorphism interaction on lipid, inflammatory and oxidative blood biomarkers

Previous investigations suggest association between obesity and Ala16Val MnSOD gene polymorphism. The V allele produces enzyme which not catalyze the superoxide anion efficiently as occurs with A allele. As obesity is related to development of other metabolic disorders we performed a study that analyzed the effect of interaction between Ala16Val MnSOD polymorphism and obesity on lipid, oxidative and inflammatory biomarkers of adult subjects. The study enrolled 161 volunteers as categorized in six groups with different genotypes: Obeses with different genotypes (AAO, VVO and AVO) and nonobese (AANO, VVNO and AVNO). In general the group AANO presented lower values whereas VVO presented higher values of biomarkers analyzed. These results suggest that oxidative metabolism influenced by genetic status could to minimize or maximize the obesity effects on lipid, oxidative and inflammatory biomarkers that are also implicated in the genesis of important dysfunctions and diseases as atherosclerosis, diabetes 2 and cardiovascular morbidities.

Salt-Induced Changes in Physio-Biochemical and Antioxidant Defense System in Mustard Genotypes

Salinity stress is a major factor limiting plant growth and productivity of many crops including oilseed.The present study investigated the identification of salt tolerant mustard genotypes and better understanding the mechanism of salinity tolerance.Salt stresses significantly reduced relative water content(RWC),chlorophyll(Chl)content,K^(+) and K^(+)/Na^(+) ratio,photosynthetic rate(P N),tran-spiration rate(Tr),stomatal conductance(gs),intercellular CO_(2) concentration(Ci)and increased the levels of proline(Pro)and lipid peroxidation(MDA)contents,Na+,superoxide(O_(2)^(•−))and hydrogen peroxide(H_(2)O_(2))in both tolerant and sensitive mustard genotypes.The tolerant genotypes maintained higher Pro and lower MDA content than the salt sensitive genotypes under stress condition.The activities of superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),glutathione peroxidase(GPX),monodehydroascorbate reductase(MDHAR)and dehydroascorbate reductase(DHAR)were increased with increasing salinity in salt tolerant genotypes,BJ-1603,BARI Sarisha-11 and BARI Sarisha-16,but the activities were unchanged in salt sensitive genotype,BARI Sarisha-14.Besides,the increment of ascorbate peroxidase(APX)activity was higher in salt sensitive genotype as compared to tolerant ones.However,the activ-ities of glutathione reductase(GR)and glutathione S-transferase(GST)were increased sharply at stress conditions in tolerant genotypes as compared to sensi-tive genotype.Higher accumulation of Pro along with improved physiological and biochemical parameters as well as reduced oxidative damage by up-regulation of antioxidant defense system are the mechanisms of salt tolerance in selected mustard genotypes,BJ-1603 and BARI Sarisha-16.

Promising Actions of Certain Medicinal Plants of Bangladesh against Diabetes and Oxidative Stress: A Review

Diabetes is a metabolic illness defined by hyperglycemia that affects 10% of the world’s population. Diabetic complications such as blindness, kidney failure, and heart failure can develop if left untreated and are made worse by oxidative stress. Oxidative stress contributes to the rise of diabetic complications, particularly type-2 diabetes. In the blood vessels of diabetic individuals, it causes endothelial dysfunction. Diabetes is one of the leading causes of death worldwide. Nowadays, it is alarming that the number of diabetic patients is increasing dramatically. There are plenty of anti-diabetic drugs available on the market, but they possess several adverse effects and do not completely cure diabetes. It has now become a financial burden on patients, their families, and society as well. Medicinal plants have gained popularity in developed and developing countries over the last two decades because of their vast natural sources and lack of harmful effects compared to modern allopathic medications. According to the World Health Organization, traditional medicines, which are mostly manufactured from plants, are still used by 80% of the population in developing countries for the management and curing of diseases. This review includes 81 Bangladeshi medicinal plants from 51 different families that can be used to treat diabetes and oxidative stress. Among them, Psidium guajava (L.), Aloe vera, Catharanthus roseus, Allium sativum, Annona squamosa, Cinnamon zeylaniucm, Amaranthus esculentus, Eugenia jambolana, Azadirachta indica, Moringa oleifera, Spondias pinnata, Coccinia grandis (L.), Momordica charantia L., Heretiera fomes, Trigonella foenum-graecum were most potent. The fundamental purpose of our study is to find out and highlight certain medicinal plants in Bangladesh that have anti-diabetic and antioxidant capabilities so that the researchers can develop newer anti-diabetic medications with minimal side effects to treat metabolic dysfunction, diabetic complications, and oxidative stress more effectively.

Oxidative stress and its complications in human health

Under normal physiological conditions, a homeostatic balance exists between the formation of oxgyen radicals (ROS) and their removal by endogenous scavenging antioxidants. An imbalance between oxidants and antioxidants that is in favor of the oxidants potentially leading to damage is termed “oxidative stress”. Mitochondria are a major producer of ROS, which mainly consists of superoxide anion, hydrogen peroxide, and hydroxyl radical. Especially, during systemic inflammatory response syndrome, sepsis, septic shock, there is an overproduction of ROS while the natural antioxidant mechanisms are weakened, mainly because of endothelial cell damage. General anesthesia can impair the immunological defense mechanisms while inducing an inflammatory reaction in alveolar macrophages. In chronic diseases and high mortality situations like sepsis, oxidative/antioxidative equilibrium shifts to the direction of oxidative stress. When elderly patients with chronic diseases and patients with sepsis undergo surgical operations, anesthesia and anesthetic agents used increases the oxidative stress in addition to the surgical trauma. Thence many studies are ongoing on the antioxidant drugs and enzymes. We must never forget to take the oxidative stress of our patients into account while planning their treatments.

CXCL10基因对SH-SY5Y细胞氧化损伤、钙稳态及Nrf2/BNIP3的影响

目的 探讨CXC趋化因子配体10(CXCL10)基因对人神经母细胞瘤细胞SH-SY5Y氧化损伤、钙稳态失衡及核因子E2相关因子2(Nrf2)、BCL2相互作用蛋白3(BNIP3)表达的影响。方法 采用H2O2损伤SH-SY5Y细胞,分为对照组(未经H2O2损伤的SH-SY5Y细胞)、模型组(H2O2干预的SH-SY5Y细胞)、NC-siRNA组(H2O2干预的SH-SY5Y细胞转染NC-siRNA)及CXCL10-siRNA组(H2O2干预的SH-SY5Y细胞转染CXCL10-siRNA),peDNA3.1.D组(H2O2干预的SH-SY5Y细胞转染空载体)及CXCLl0/peDNA3.1组(H2O2干预的SH-SY5Y细胞转染CXCL10/peDNA3.1)。采用实时荧光定量(qRT-PCR)检测各组细胞CXCL10 mRNA、MTT检测各组细胞活性、相关试剂盒检测氧化应激指标、流式细胞仪检测各组细胞Ca2+荧光强度和免疫印迹检测各组细胞Nrf2、BNIP3表达。结果 对照组、模型组、NC-siRNA组、CXCL10-siRNA组、peDNA3.1.D组和CXCLl0/peDNA3.1组中CXCL10 mRNA水平分别为1.02±0.08、1.44±0.14、1.40±0.17、0.73±0.05、1.49±0.18和2.20±0.16,差异有统计学意义(F=77.400,P<0.05);与对照组相比,模型组细胞活性、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)、Nrf2、BNIP3降低,丙二醛(MDA)、Ca2+荧光强度升高(P<0.05);模型组、NC-siRNA组、peDNA3.1.D组上述指标比较无意义(P>0.05),与模型组相比,CXCL10-siRNA组细胞活性、GSH、SOD、CAT、Nrf2、BNIP3升高(P<0.05),MDA、Ca2+荧光强度升降低(P<0.05),CXCL10/peDNA3.1组细胞活性、GSH、SOD、CAT、Nrf2、BNIP3降低(P<0.05),MDA、Ca2+荧光强度升高(P<0.05)。结论 下调CX-CL10基因可提高H2O2损伤后SH-SY5Y细胞活性,并可抗氧化应激,降低细胞内游离Ca2+水平,这可能与激活Nrf2/BNIP3信号通路相关。

高原低氧诱导肠稳态失衡的分子机制及中药防治研究进展

低氧是限制人群在高原生存的因素之一,能够引起呕吐腹泻、心慌气短、急性昏迷等症状。患有急性高原病的人约80%至少有一种胃肠道不适的症状,如厌食、恶心、腹泻、呕吐等。研究高原低氧所致肠损伤的病理特点、发病机制及药物防治,有利于高原胃肠道疾病的诊治。因此,本文通过总结相关文献,就高原低氧造成肠道损伤的相关研究进行系统阐述,对高原低氧造成肠道形态学、肠道细胞、肠道菌群等肠稳态的改变,引起肠道炎症、氧化损伤等机制及中药防治进行总结,以期为科研工作者和临床工作者提供借鉴及信息参考。

真武汤联合缩泉丸加减对糖尿病肾病Ⅲ期(脾肾阳虚型)患者的临床研究

目的探讨真武汤和缩泉丸加减对糖尿病肾病Ⅲ期(脾肾阳虚型)患者的影响。方法纳入128例糖尿病肾病Ⅲ期(脾肾阳虚型)患者,随机分成观察组和对照组,对照组采用西医常规治疗,观察组联合真武汤和缩泉丸加减治疗,治疗12周后比较两组疗效。结果治疗后,观察组血清转化生长因子-β1(TGF-β1)、糖原合酶激酶-3β(GSK-3β)、白介素-6(IL-6)水平、血清肌酐(Scr)、尿素氮(BUN)、24 h尿蛋白、中医症状总积分低于对照组(t分别=9.84、8.39、11.12、8.83、7.53、8.58、30.76,P均<0.05),而血清血红素加氧酶(HO-1)、氧化物歧化酶(SOD)、白介素-10(IL-10)水平高于对照组(t分别=7.74、19.03、9.58,P均<0.05)。治疗后观察组健康状况调查简表(SF-36)评分高于对照组(t=5.59,P<0.05),总有效率高于对照组(χ^(2)=8.18,P<0.05)。结论对于糖尿病肾病Ⅲ期(脾肾阳虚型)患者而言联合真武汤和缩泉丸加减效果显著,可减轻氧化应激失衡,促进Th1/Th2平衡恢复,促进肾功能恢复,提高临床疗效。