Dyslipidemia is a condition in which the lipid levels are altered in the body.The fixed oils(FOs)from spices,Alpinia galanga,Cinnamomum zeylanicum,Trigonella foenum-graecum,Foeniculum vulgare,and Myristica fragrans we...Dyslipidemia is a condition in which the lipid levels are altered in the body.The fixed oils(FOs)from spices,Alpinia galanga,Cinnamomum zeylanicum,Trigonella foenum-graecum,Foeniculum vulgare,and Myristica fragrans were evaluated as potential sources of anti-dyslipidemic activity.The nutraceutical compound fractions such as polyphenols,tocopherols,γ-oryzanol,and phytosterols were extracted from spice FOs,tested individually and in combination for cytotoxicity and their effect on lipid accumulation in 3T3-L1 and Hep-G2 cell lines.Further,the best performing FOs in the in vitro studies were fed to high fat diet-induced dyslipidemic C57BL/6 mice and studied its activity in the liver and adipose tissue.The in vitro assays showed no cytotoxicity in the studied FOs up to 200μg/mL concentration.Out of all the studied nutraceutical compound fractions,theγ-oryzanol and phytosterols fractions showed a reduction in the accumulation of lipid droplets in 3T3-L1 cell lines.Moreover,the reconstituted nutraceutical fractions from C.zeylanicum,T.foenum-graecum,and F.vulgare FOs exhibited a dose dependent inhibition of lipid accumulation compared to the fractions of A.galanga and M.fragrans FOs.The in vivo studies confirmed the anti-dyslipidemic activity of C.zeylanicum FO that showed an improved lipid profile,antioxidant enzymes,and reduced lipid droplets in the liver and adipose tissue.The histopathological images of the liver and adipose tissue showed a comparable visualisation in Atorvastatin,and C.zeylanicum fed group mice.Hence,the spice FOs are potentially efficacious anti-dyslipidemic functional food ingredients and could be safer than statins.展开更多
基金The author(VM)is thankful to the Indian Council of Medical Research,New Delhi,India,for the award of ICMR-SRF.The authors greatly acknowledge the Director,CSIR-CFTRI,for her constant support and encouragement.Part of this work was supported by SERB,New Delhi(ECR/2016/001548),India.
文摘Dyslipidemia is a condition in which the lipid levels are altered in the body.The fixed oils(FOs)from spices,Alpinia galanga,Cinnamomum zeylanicum,Trigonella foenum-graecum,Foeniculum vulgare,and Myristica fragrans were evaluated as potential sources of anti-dyslipidemic activity.The nutraceutical compound fractions such as polyphenols,tocopherols,γ-oryzanol,and phytosterols were extracted from spice FOs,tested individually and in combination for cytotoxicity and their effect on lipid accumulation in 3T3-L1 and Hep-G2 cell lines.Further,the best performing FOs in the in vitro studies were fed to high fat diet-induced dyslipidemic C57BL/6 mice and studied its activity in the liver and adipose tissue.The in vitro assays showed no cytotoxicity in the studied FOs up to 200μg/mL concentration.Out of all the studied nutraceutical compound fractions,theγ-oryzanol and phytosterols fractions showed a reduction in the accumulation of lipid droplets in 3T3-L1 cell lines.Moreover,the reconstituted nutraceutical fractions from C.zeylanicum,T.foenum-graecum,and F.vulgare FOs exhibited a dose dependent inhibition of lipid accumulation compared to the fractions of A.galanga and M.fragrans FOs.The in vivo studies confirmed the anti-dyslipidemic activity of C.zeylanicum FO that showed an improved lipid profile,antioxidant enzymes,and reduced lipid droplets in the liver and adipose tissue.The histopathological images of the liver and adipose tissue showed a comparable visualisation in Atorvastatin,and C.zeylanicum fed group mice.Hence,the spice FOs are potentially efficacious anti-dyslipidemic functional food ingredients and could be safer than statins.
