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Effects of Oxidized Low Density Lipoprotein on Transformation of Valvular Myofibroblasts to Osteoblast-like Phenotype 被引量:2
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作者 陈娣 沈迎念 +2 位作者 胡伟林 陈正平 李永胜 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2015年第3期362-367,共6页
In order to investigate the roles of Wnt signal pathway in transformation of cardiac valvular myofibroblasts to the osteoblast-like phenotype, the primary cultured porcine aortic valve myofibroblasts were incubated wi... In order to investigate the roles of Wnt signal pathway in transformation of cardiac valvular myofibroblasts to the osteoblast-like phenotype, the primary cultured porcine aortic valve myofibroblasts were incubated with oxidized low density lipoprotein(ox-LDL, 50 mg/L), and divided into four groups according to the ox-LDL treatment time: control group, ox-LDL 24-h group, ox-LDL 48-h group, and ox-LDL 72-h group. Wnt signal pathway blocker Dickkopf-1(DDK-1, 100 μg/L) was added in ox-LDL 72-h group. The expression of α-smooth muscle actin(α-SMA), bone morphogenetic protein 2(BMP2), alkaline phosphatase(ALP), and osteogenic transcription factor Cbfa-1 was detected by Western blotting, and that of β-catenin, a key mediator of Wnt signal pathway by immunocytochemical staining method. The Wnt/β-catenin was observed and the transformation of myofibroblasts to the osteoblast-like phenotype was examined. The expression of α-SMA, BMP2, ALP and Cbfa-1 proteins in the control group was weaker than in the ox-LDL-treated groups. In ox-LDL-treated groups, the protein expression of α-SMA, BMP2, ALP, and Cbfa-1 was significantly increased in a time-dependent manner as compared with the control group, and there was significant difference among the three ox-LDL-treated groups(P〈0.05 for all); β-catenin protein was also up-regulated in the ox-LDL-treated groups in a time-dependent manner as compared with the control group(P〈0.05), and its transfer from cytoplasm to nucleus and accumulation in the nucleus were increased in the same fashion(P〈0.05). After addition of DKK-1, the expression of α-SMA, bone-related proteins and β-catenin protein was significantly reduced as compared with ox-LDL 72-h group(P〈0.05). The Wnt/ β-catenin signaling pathway may play an important role in transformation of valvular myofibroblasts to the osteoblast-like phenotype. 展开更多
关键词 oxidized low density lipoprotein cardiac valve calcification MYOFIBROBLASTS WNT/Β-CATENIN OSTEOBLASTS
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Role of PERK/eIF2α/CHOP Endoplasmic Reticulum Stress Pathway in Oxidized Low-density Lipoprotein Mediated Induction of Endothelial Apoptosis 被引量:21
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作者 TAO Yong Kang YU Pu Lin +3 位作者 BAI Yong Ping YAN Sheng Tao ZHAO Shui Ping ZHANG Guo Qiang 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2016年第12期868-876,共9页
Objective PERK/elF2/CHOP is a major signaling pathway mediating endoplasmic reticulum (ER) stress related with atherosclerosis. Oxidized LDL (ox-LDL) also induces endothelial apoptosis and plays a vital role in th... Objective PERK/elF2/CHOP is a major signaling pathway mediating endoplasmic reticulum (ER) stress related with atherosclerosis. Oxidized LDL (ox-LDL) also induces endothelial apoptosis and plays a vital role in the initiation and progression of atherosclerosis. The present study was conducted to explore the regulatory effect of ox-LDL on PERK/elF2a/CHOP signaling pathway in vascular endothelial cells. Methods The effects of ox-LDL on PERK and p-elF2a protein expression of primary human umbilical vein endothelial cells (HUVECs) were investigated by Western blot analysis. PERK gene silencing and selective elF2a phosphatase inhibitor, salubrinal were used to inhibit the process of ox-LDL induced endothelial cell apoptosis, caspase-3 activity, and CHOP mRNA level. Results Ox-LDL treatment significantly increased the expression of PERK, PERK-mediated inactivation of elF2a phosphorylation, and the expression of CHOP, as well as the caspase-3 activity and apoptosis. The effects of ox-LDL were markedly decreased by knocking down PERK with stable transduction of lentiviral shRNA or by selective elF2a phosphatase inhibitor, salubrinal. Conclusion This study provides the first evidence that ox-LDL induces apoptosis in vascular endothelial cells mediated largely via the PERK/elF2a/CHOP ER-stress pathway. It adds new insights into the molecular mechanisms underlying the pathogenesis and progression of atherosclerosis. 展开更多
关键词 PERK elF2a CHOP Endoplasmic reticulum stress oxidized low-density lipoprotein Endothelial cell Apoptosis ATHEROSCLEROSIS Caspase-3
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Expression of Monocyte Chemoattractant Protein-1 in Monocytes and Effects of Native and Oxidized Very Low Density Lipoproteins 被引量:1
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作者 王国平 邓仲端 倪娟 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1997年第4期203-205,共3页
Monocyte chemoattractant protein-1(MCP-1), a potent chemoattractant, is thought to play an important role in migration of monocytes into atherosclerotic lesions. The present study was designed to investigate the capac... Monocyte chemoattractant protein-1(MCP-1), a potent chemoattractant, is thought to play an important role in migration of monocytes into atherosclerotic lesions. The present study was designed to investigate the capacity of human peripheral blood monocytes to express MCP-1 and effects of native very low density lipoprotein (VLDL) and oxidized VLDL(OX-VLDL) on the expression. The total RNA was extracted from cultured monocytes, which were exposed to VLDL and OX-VLDL, and the media conditioned by monocytes were collected. MCP-1 mRNA expression was examined by Northern blot analysis. MCP-1 protein in conditioned media was determined by using sandwich ELISA. The results showed that monocytes can express MCP-1 after a 24 h incubation at 37℃,and the expression was markedly increased by a exposure to OX-VLDL, whereas the expression was slightly increased when exposed to VLDL. It suggests that the capacity of monocytes to produce MCP-1 that recruits and activates circulating monocytes may be of considerable importance in atherogenesis, and oxidation of VLDL enhances its potential to promote atherogenesis. 展开更多
关键词 monocyte chemoattractant protein-1 very low density lipoprotein oxidIZATION MONOCYTES ATHEROSCLEROSIS
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Anti-oxidized low-density lipoprotein antibodies in chronic heart failure 被引量:2
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作者 Gideon Charach Alexander Rabinovich +4 位作者 Ori Argov Moshe Weintraub Lior Charach Oded Ayzenberg Jacob George 《World Journal of Cardiology》 CAS 2012年第11期302-308,共7页
Oxidative stress may play a significant role in the pathogenesis of heart failure(HF).Antibodies to oxidized low-density lipoprotein(oxLDL Abs) reflect an immune response to LDL over a prolonged period and may represe... Oxidative stress may play a significant role in the pathogenesis of heart failure(HF).Antibodies to oxidized low-density lipoprotein(oxLDL Abs) reflect an immune response to LDL over a prolonged period and may represent long-term oxidative stress in HF.The oxLDL plasma level is a useful predictor of mortality in HF patients,and measurement of the oxLDL Abs level may allow better management of those patients.Antibodies to oxLDL also significantly correlate with the New York Heart Association score.Hypercholesterolemia,smoking,hypertension,and obesity are risk factors for atherosclerotic coronary heart disease(CHD) leading to HF,but these factors account for only onehalf of all cases,and understanding of the pathologic process underlying HF remains incomplete.Nutrients with antioxidant properties can reduce the susceptibility of LDL to oxidation.Antioxidant therapy may be an adjunct to lipid-lowering,angiotensin converting enzyme inhibition and metformin(in diabetes) therapy for the greatest impact on CHD and HF.Observational data suggest a protective effect of antioxidant supple-mentation on the incidence of HD.This review summarizes the data on oxLDL Abs as a predictor of morbidity and mortality in HF patients. 展开更多
关键词 HEART failure oxidIZED low-density lipoproteinS ANTIBODIES ANTIoxidANTS
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Oxidized low density lipoprotein (Ox-LDL) impacts on erythrocyte viscoelasticity and its molecular mechanism 被引量:1
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作者 K.-L.Paul Sung Lanping Amy Sung 《医用生物力学》 EI CAS CSCD 2009年第S1期60-60,共1页
Aim:The oxidized low-density lipoprotein(OxLDL) plays an important role in atherosclerosis yet it remains unclear if it damages circulating erythrocytes. Method: In this study。
关键词 OX-LDL impacts on erythrocyte viscoelasticity and its molecular mechanism oxidized low density lipoprotein
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Inhibition of Oxidative Modification of Human Low Density Lipoprotein by Resveratrol
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作者 邹建刚 黄元铸 +2 位作者 陈琪 魏恩会 曹克将 《The Journal of Biomedical Research》 CAS 1999年第1期7-11,22,共6页
To further investigate whether resveratrol, a polyphenolic compound in red wine, affects the oxidation of human low density lipoprotein (LDL), LDL purified from normolipidemic subjects was subjected to Cu\+\{2+\}induc... To further investigate whether resveratrol, a polyphenolic compound in red wine, affects the oxidation of human low density lipoprotein (LDL), LDL purified from normolipidemic subjects was subjected to Cu\+\{2+\}induced or azo compoundinitiated oxidative modification. The extent of LDL modification was assessed by measuring the formation of thiobarbituric acid reactive substances (TBARS) and the relative electrophoretic mobilities (REM) of LDL. Resveratrol (50 mol/L) reduced TBARS and REM of LDL during Cu\+\{2+\}induced oxidation by 70.5% and 42.3%, respectively (P<0.01), and prolonged the lag phase associated with the oxidative modification of LDL by copper ion or azo compound. These in vitro results suggest that resveratrol may afford LDL protection against oxidative modification, possibly by acting as a free radical scavenger. 展开更多
关键词 RESVERATROL ANTIoxidANT oxidation low density lipoprotein CHOLESTEROL
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Influence of Oxidized Low Density Lipoprotein on the Proliferation of Human Artery Smooth Muscle Cells in vitro 被引量:5
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作者 乔晨晖 张凯伦 夏家红 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第1期20-23,共4页
The effects of oxidized low density lipoprotein (ox-LDL) on the proliferation of cultured human vascular smooth muscle cells (vSMC) were investigated in vitro. By using NaBr density gradient centrifugation, LDL wa... The effects of oxidized low density lipoprotein (ox-LDL) on the proliferation of cultured human vascular smooth muscle cells (vSMC) were investigated in vitro. By using NaBr density gradient centrifugation, LDL was isolated and purified from human plasma. Ox-LDL was produced from LDL by being incubated with CuSO4. ox-LDL was then added to the culture medium at different concentrations (35, 60, 85, 110, 135 and 160μg/mL) for 7 days. The influence of ox-LDL on vSMC proliferation was observed in growth curve, mitosis index, and in situ determination of apoptosis. The data were analyzed with SPSS 10.0 software. The results showed that the ox-LDL produced in vitro had a good purity and optimal oxidative degree, which was similar to the intrinsic ox-LDL in atherosclerotic plaque, ox-LDL at a concentration of 35 μg/mL demonstrated the strongest proliferation inducement, and at a concentration of 135 μg/mL, ox-LDL could inhibit the growth of vSMC. ox-LDL at concentrations of 35 and 50 μg/mL presented powerful mitotic trigger, and with the increase of ox-LDL concentration, the mitotic index of vSMC was decreased gradually, ox-LDL at higher concentrations promoted more apoptotic vSMCs, ox-LDL at lower concentrations triggered proliferation of vSMCs, and at higher concentrations induced apoptosis in vSMCs, ox-LDL played a promotional role in the pathogenesis and development of atherosclerosis by affecting vSMC proliferation and apoptosis. 展开更多
关键词 oxidized low density lipoprotein smooth muscle cell PROLIFERATION ATHEROSCLEROSIS
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Obstructive jaundice leads to accumulation of oxidized low density lipoprotein in human liver tissue 被引量:1
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作者 Mustafa Comert Yucel Ustundag +2 位作者 Ishak Ozel Tekin Banu Dogan Gun Figen Barut 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第31期5094-5095,共2页
Oxidized low density lipoprotein (ox-LDL) molecule is one of the most important modified lipoproteins produced during the oxidative stress. Modified lipoproteins have been defined as being part of the immune inflamm... Oxidized low density lipoprotein (ox-LDL) molecule is one of the most important modified lipoproteins produced during the oxidative stress. Modified lipoproteins have been defined as being part of the immune inflammatory mechanisms in association with oxidant stress. We have reported the accumulation of ox-LDL in Balb/c mice liver after bile duct ligation previously. Here, we investigated this finding in human beings with obstructive jaundice. Our study demonstrates that obstructive jaundice results in tremendous accumulation of ox-LDL in the liver tissue of patients. 展开更多
关键词 Obstructive jaundice LIVER oxidative stress oxidized low density lipoprotein
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EC,ASMC and Macrophage Oxidize Human Low Density Lipoprotein
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作者 Dai Zhao\|ming, Wu Jun\|zhu, Li Xiao\| ming, Chen Li\|da, Hong Jia\|ling Department of Biochemistry, Medical College, Wuhan University, Wuhan 430071 ,Hubei, China 《Wuhan University Journal of Natural Sciences》 CAS 2003年第01A期130-134,共5页
To investigate the mechanism of LDL oxidation i n vivo , LDL was incubated with endothelium cell (EC),artery smooth muscle cel l (ASMC) and macrophage, and then the change of myeloperoxidase (MPO) activity i n ce... To investigate the mechanism of LDL oxidation i n vivo , LDL was incubated with endothelium cell (EC),artery smooth muscle cel l (ASMC) and macrophage, and then the change of myeloperoxidase (MPO) activity i n cell and medium and the oxidation of LDL by those three cells were assessed. T he result showed that LDL promoted the activity of cellular and secretive myelop eroxidase which was concentration\|dependent on LDL; with elevation of MPO activ ity, oxidation of LDL intensified, which was expressed by the formation of conju gated dienes and the elevation of thiobarbituric acid teactive substance (TBARS ). Macrophage's MPO activity went up with the increase of LDL at both low and h igh concentration; EC's MPO activity went up with the increase of LDL only at h igh concentration and ASMC's MPO activity wasn't sensitive to LDL concentratio n change. The results suggest that Macrophage might be crucial to the oxidation of LDL in vivo , in which MPO might play an important role. 展开更多
关键词 low density lipoprotein oxidATION in vivo MYELOPERoxidASE
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Protein in oxidized low density lipoprotein may cause injury to mitochondria of mouse peritoneal macrophages
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作者 刘尚喜 周玫 +1 位作者 陈瑗 文维延 《Journal of Medical Colleges of PLA(China)》 CAS 1997年第4期265-268,272,共5页
Injury to mitochondria of macrophages caused by oxidized low density lipoprotein (Ox-LDL) and the role of lipid hydroperoxides (LOOH). lipid and protein in Ox-LDL on the injury were studied by measuring mito-chondrial... Injury to mitochondria of macrophages caused by oxidized low density lipoprotein (Ox-LDL) and the role of lipid hydroperoxides (LOOH). lipid and protein in Ox-LDL on the injury were studied by measuring mito-chondrial membrane potential (MMP) on ACAS570. The results showed that MMP decreased when macrophageswere treated by Ox-LDL. If LOOH in Ox-LDL was pre cleared by ebselen plus GSH. the decreased MMP could be recovered by about 20 %. Lipid moiety alone had no effect on IMP, but protein moiety could cause decrease ofMMP, the extent of the decrease was equivalent to that caused by Ox-LDL in which LOOH was pre-cleared by ebselen plus GSH. 展开更多
关键词 oxidIZED low density lipoprotein lipid HYDROPERoxidE APOB MACROPHAGES mitochondron mitochondrial membrane potential
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基于ox-LDL/LOX-1信号通路探讨脂质代谢紊乱促进肺癌进展中的机制
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作者 吴阳 姚坚 陈金亮 《实用医学杂志》 CAS 北大核心 2024年第1期19-24,31,共7页
目的基于氧化低密度脂蛋白(ox-LDL)/人凝集素样氧化低密度脂蛋白受体1(LOX-1)信号通路探讨脂质代谢紊乱促进肺癌进展的机制。方法收集81个已鉴定的具有成对相邻非癌组织(离肿瘤至少5 cm)的肺腺癌组织,使用免疫组织化学检测LOX-1表达。... 目的基于氧化低密度脂蛋白(ox-LDL)/人凝集素样氧化低密度脂蛋白受体1(LOX-1)信号通路探讨脂质代谢紊乱促进肺癌进展的机制。方法收集81个已鉴定的具有成对相邻非癌组织(离肿瘤至少5 cm)的肺腺癌组织,使用免疫组织化学检测LOX-1表达。肺腺癌细胞系(A549、H1299细胞)中过表达LOX-1。用Transwell测定细胞侵袭能力。用不同浓度oxLDL处理细胞,并检测细胞中LOX-1表达情况。结果在包含原发性人肺癌和匹配的邻近非癌组织中,肿瘤中LOX-1染色比非癌组织样品明显增强(中值H分数99.4 vs.16.2,P<0.001)。高LOX-1表达与低生存显著相关(P<0.001)。与无淋巴结转移的患者相比,发生淋巴结转移患者的癌组织具有更高的LOX-1水平(中值H分数83.2 vs.121.1,P<0.01)。LOX-1过表达显著促进肺癌细胞的侵袭转移细胞数(P<0.01)。此外,LOX-1是ox-LDL诱导的肺癌细胞转移所必需的功能靶点。伊他替尼抑制LOX-1过表达的A549在体外的转移能力。结论LOX-1的表达随着oxLDL水平的升高而增加,并且LOX-1的表达上调促进了肺癌细胞的转移,其作用机制可能与激活Janus激酶/转录因子激活子(JAK1/STAT6)信号通路有关。 展开更多
关键词 氧化低密度脂蛋白 人凝集素样氧化低密度脂蛋白受体1 肺癌 脂质代谢
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外源性GDF11通过调控LOX-1介导的内质网应激途径改善高血压大鼠血管重构
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作者 戴正东 胡良坤 +1 位作者 万伟 徐丽新 《中国动脉硬化杂志》 CAS 2024年第10期850-856,共7页
[目的]探讨外源性生长分化因子11(GDF11)通过调控凝集素样氧化型低密度脂蛋白受体1(LOX-1)介导的内质网应激途径对高血压大鼠血管重构的影响。[方法]将大鼠随机分为对照组、模型组(AngⅡ诱导的高血压大鼠模型组)、r-GDF11组、Ab-LOX-1组... [目的]探讨外源性生长分化因子11(GDF11)通过调控凝集素样氧化型低密度脂蛋白受体1(LOX-1)介导的内质网应激途径对高血压大鼠血管重构的影响。[方法]将大鼠随机分为对照组、模型组(AngⅡ诱导的高血压大鼠模型组)、r-GDF11组、Ab-LOX-1组和r-GDF11+r-LOX-1组,每组10只。采用动物无创血压仪检测大鼠尾动脉血压变化;HE染色评估主动脉血管形态;Masson染色评估主动脉组织胶原沉积情况;Western blot检测主动脉组织中GDF11、LOX-1及内质网应激相关蛋白表达。[结果]与对照组比较,模型组大鼠血压升高,主动脉组织中膜厚度(MT)、MT/管腔内径(LD)比值增大,LD减小(均P<0.05);主动脉组织胶原纤维容积分数(CVF)值、葡萄糖调节蛋白78(GRP78)和转录激活因子6(ATF6)蛋白表达、磷酸化PKR样内质网调节激酶(p-PERK)/PERK和磷酸化肌醇需求酶1α(p-IRE1α)/IRE1α比值均升高(均P<0.05)。与模型组比较,r-GDF11组和Ab-LOX-1组大鼠血压降低,主动脉组织MT、MT/LD均减小,LD增大(均P<0.05);主动脉组织CVF值、GRP78和ATF6蛋白表达、p-PERK/PERK和p-IRE1α/IRE1α比值均显著降低(均P<0.05)。与r-GDF11组比较,r-GDF11+r-LOX-1组大鼠血压升高,主动脉组织MT、MT/LD增大,LD减小(均P<0.05);主动脉组织CVF值、GRP78和ATF6蛋白表达、p-PERK/PERK和p-IRE1α/IRE1α比值显著升高(均P<0.05)。[结论]外源性GDF11通过抑制LOX-1介导的内质网应激途径改善高血压大鼠血管重构。 展开更多
关键词 外源性生长分化因子11 高血压 血管重构 凝集素样氧化型低密度脂蛋白受体1 内质网应激
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微小RNA-26a-5p对氧化型低密度脂蛋白处理的血管平滑肌细胞增殖、迁移和泡沫化的影响
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作者 赵妙惠 周佳妮 吴超群 《现代实用医学》 2024年第4期428-432,共5页
目的探究微小RNA-26a-5p(miR-26a-5p)对动脉粥样硬化(AS)过程中血管平滑肌细胞(VSMCs)增殖、迁移和泡沫化的影响。方法使用氧化型低密度脂蛋白(ox-LDL)处理VSMCs,模拟AS过程中的VSMCs细胞模型,向细胞中转染miR-26a-5p模拟物(miR-26a-5p ... 目的探究微小RNA-26a-5p(miR-26a-5p)对动脉粥样硬化(AS)过程中血管平滑肌细胞(VSMCs)增殖、迁移和泡沫化的影响。方法使用氧化型低密度脂蛋白(ox-LDL)处理VSMCs,模拟AS过程中的VSMCs细胞模型,向细胞中转染miR-26a-5p模拟物(miR-26a-5p mimic)及其阴性对照(NC mimic),将细胞分为对照组、ox-LDL组、ox-LDL+NC mimic组、ox-LDL+miR-26a-5p mimic组。通过qRT-PCR检测细胞miR-26a-5p表达水平,采用CCK-8和EDU染色检测细胞活力和增殖,通过划痕实验和Transwell实验检测细胞迁移和侵袭能力,使用油红O染色观察细胞中脂滴聚集情况,采用免疫荧光实验检测细胞中α-SMA的表达。结果VSMCs中miR-26a-5p水平随着ox-LDL浓度的增加和作用时间的延长而降低。与对照组比较,ox-LDL组VSMCs细胞增殖、迁移、侵袭能力显著增强,脂滴聚集增多,α-SMA信号减弱,泡沫化程度增强。与oxLDL+NC mimic组比较,ox-LDL+miR-26a-5p mimic组细胞增殖、迁移、侵袭能力显著减弱,脂滴聚集减少,α-SMA信号增强,泡沫化程度减弱。结论过表达miR-26a-5p可以下调血管平滑肌细胞的增殖、迁移和泡沫化,miR-26a-5p可能成为AS治疗的新靶点。 展开更多
关键词 动脉粥样硬化 miR-26a-5p OX-LDL VSMCS 泡沫细胞
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血清S-100B蛋白、可溶性凝集素样氧化低密度脂蛋白受体-1、胶质纤维酸性蛋白检测在新生儿缺血缺氧性脑病病情严重程度中的诊断价值
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作者 耿淑霞 《陕西医学杂志》 CAS 2024年第1期118-121,共4页
目的:探讨血清S-100B蛋白、可溶性凝集素样氧化低密度脂蛋白受体-1(sLOX-1)、胶质纤维酸性蛋白(GFAP)与新生儿缺血缺氧性脑病(HIE)病情严重程度的关系。方法:选择80例HIE患儿作为观察组,另选择90例健康新生儿作为对照组,收集所有患儿一... 目的:探讨血清S-100B蛋白、可溶性凝集素样氧化低密度脂蛋白受体-1(sLOX-1)、胶质纤维酸性蛋白(GFAP)与新生儿缺血缺氧性脑病(HIE)病情严重程度的关系。方法:选择80例HIE患儿作为观察组,另选择90例健康新生儿作为对照组,收集所有患儿一般资料,并检测两组患儿血清S-100B蛋白、sLOX-1、GFAP水平,分析HIE患儿血清S-100B蛋白、sLOX-1、GFAP与病情严重程度的相关性及预后不良的影响因素。结果:对照组血清S-100B蛋白、sLOX-1、GFAP水平低于观察组(均P<0.05)。重度组血清S-100B蛋白、sLOX-1、GFAP水平高于中度组、轻度组和对照组(均P<0.05)。Pearson相关分析显示,疾病严重程度与HIE患儿血清S-100B蛋白、sLOX-1、GFAP水平呈正相关(均P<0.001)。随访预后良好患儿59例,预后不良21例,经多因素Logistic回归分析显示,产程异常、病情重度、S-100B蛋白、sLOX-1、GFAP为影响HIE患儿预后的危险因素(均P<0.05)。结论:HIE患儿病情严重程度和预后与血清S-100B蛋白、sLOX-1、GFAP水平有关,监测其水平变化有利于临床早期完善干预方案改善预后。 展开更多
关键词 S-100B蛋白 可溶性凝集素样氧化低密度脂蛋白受体-1 胶质纤维酸性蛋白 新生儿缺血缺氧性脑病 相关性 预后
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血清KLK6、CCR2、ox-LDL水平与帕金森病的相关性研究
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作者 马晓琳 胡兆婷 +3 位作者 郑伟 崔晓 张真 许谦 《国际检验医学杂志》 CAS 2024年第5期614-617,623,共5页
目的分析血清激肽释放酶6(KLK6)、CC趋化因子受体2(CCR2)、氧化修饰低密度脂蛋白(ox-LDL)与帕金森病的相关性。方法将2020年7月至2022年12月于该院诊治的帕金森病患者150例纳入研究作为患者组,按照Hoehn-Yahr(H-Y)分期进一步分为Ⅰ期27... 目的分析血清激肽释放酶6(KLK6)、CC趋化因子受体2(CCR2)、氧化修饰低密度脂蛋白(ox-LDL)与帕金森病的相关性。方法将2020年7月至2022年12月于该院诊治的帕金森病患者150例纳入研究作为患者组,按照Hoehn-Yahr(H-Y)分期进一步分为Ⅰ期27例、Ⅱ期42例、Ⅲ期47例、Ⅳ期34例。另外,选取同期健康体检者150例作为对照组。采用简易精神状态检查(MMSE)量表评估患者精神障碍情况。比较患者组与对照组及不同H-Y分期帕金森病患者血清KLK6、CCR2、ox-LDL水平。分析血清KLK6、CCR2、ox-LDL对帕金森病的诊断价值。分析血清KLK6、CCR2、ox-LDL与H-Y分期、MMSE评分的相关性。结果患者组血清KLK6、CCR2、ox-LDL水平高于对照组(P<0.05)。受试者工作特征(ROC)曲线分析显示,KLK6、CCR2、ox-LDL诊断帕金森病的曲线下面积(AUC)分别为0.813、0.847、0.826,最佳临界值对应的灵敏度、特异度:KLK6为66.7%、90.0%,CCR2为68.0、91.3%,ox-LDL为59.3%、100.0%。不同H-Y分期患者血清KLK6、CCR2、ox-LDL比较:Ⅰ期<Ⅱ期<Ⅲ期<Ⅳ期;MMSE评分比较:Ⅰ期>Ⅱ期>Ⅲ期>Ⅳ期;两两比较差异均有统计学意义(P<0.05)。血清KLK6、CCR2、ox-LDL水平与H-Y分期均呈正相关(r=0.559、0.716、0.722,P<0.05);血清KLK6、CCR2、ox-LDL水平与MMSE评分均呈负相关(r=-0.276、-0.448、-0.457,P<0.05)。结论血清KLK6、CCR2、ox-LDL对帕金森病患者具有一定的诊断价值,并且与帕金森病患者的病情严重程度和认知功能有关。 展开更多
关键词 帕金森病 激肽释放酶6 CC趋化因子受体2 氧化修饰低密度脂蛋白 相关性 预测价值
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恩格列净通过调控AMPK/eNOS信号通路改善ox-LDL诱导的内皮祖细胞功能障碍
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作者 帅青云 张晶 +3 位作者 唐光能 赵祺 曹政 涂强 《西部医学》 2024年第5期667-673,共7页
目的研究恩格列净(EMP)对氧化低密度脂蛋白(ox-LDL)诱导内皮祖细胞(EPCs)损伤的保护作用及机制。方法通过密度梯度离心法提取、分离并培养小鼠骨髓来源的的EPCs。采用Dil标记乙酰化低密度脂蛋白(Dil-ac-LDL)联合FITC标记荆豆凝集素-1(FI... 目的研究恩格列净(EMP)对氧化低密度脂蛋白(ox-LDL)诱导内皮祖细胞(EPCs)损伤的保护作用及机制。方法通过密度梯度离心法提取、分离并培养小鼠骨髓来源的的EPCs。采用Dil标记乙酰化低密度脂蛋白(Dil-ac-LDL)联合FITC标记荆豆凝集素-1(FITC-UEA-1)双摄取法鉴定。将EPCs分为正常对照组,ox-LDL组以及ox-LDL联合不同浓度恩格列净实验组。CCK-8检测细胞活力,Transwell检测细胞迁移,FITC-Annexin V/PI检测细胞凋亡,ELISA检测细胞上清液中血管内皮细胞生长因子(VEGF)、基质细胞衍生因子-1α(SDF-1α)含量;流式细胞术检测一氧化氮(NO)的合成情况。Western blot检测AMPK、p-AMPK、eNOS、p-eNOS的蛋白表达。结果提取的EPCs诱导培养至第7天经鉴定为小鼠骨髓EPCs。与对照组比较,ox-LDL组细胞活力降低,迁移细胞减少,凋亡增加,VEGF、SDF-1α含量降低,NO合成减少(P<0.05);与ox-LDL组相比,不同浓度恩格列净组细胞活力有所提高,迁移细胞增多,凋亡减少,VEGF、SDF-1α含量升高,NO合成增加(P<0.05)。ox-LDL处理可明显抑制AMPK及eNOS磷酸化(P<0.05),恩格列净处理可以改善AMPK及eNOS磷酸化水平(P<0.05),而AMPK抑制剂Compound C可使恩格列净改善EPCs功能活性的作用受到明显的抑制(P<0.05)。结论恩格列净可改善ox-LDL诱导的EPCs功能障碍,其机制与调控AMPK/eNOS信号通路有关。 展开更多
关键词 恩格列净 氧化低密度脂蛋白 内皮祖细胞 一氧化氮 血管新生
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隔药饼灸对动脉粥样硬化兔血清Ox-LDL、IFN-γ表达的影响
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作者 易洪芬 陈昕羽 +4 位作者 彭涵 肖孟霞 欧阳里知 刘红华 刘迈兰 《湖南中医药大学学报》 CAS 2024年第9期1614-1619,共6页
目的 观察隔药饼灸对动脉粥样硬化(atherosclerosis,AS)兔血清氧化低密度脂蛋白(oxidized low-density lipoprotein,Ox-LDL)、干扰素(interferon-γ,IFN-γ)的影响,探讨隔药饼灸抗AS的作用机制。方法 将18只新西兰兔随机分为正常组、模... 目的 观察隔药饼灸对动脉粥样硬化(atherosclerosis,AS)兔血清氧化低密度脂蛋白(oxidized low-density lipoprotein,Ox-LDL)、干扰素(interferon-γ,IFN-γ)的影响,探讨隔药饼灸抗AS的作用机制。方法 将18只新西兰兔随机分为正常组、模型组、隔药饼灸组,每组6只。正常组给予普通饲料喂养,其余2组给予高脂饲料喂养,其中隔药饼灸组边造模边干预:2组穴位(巨阙、天枢、丰隆;心俞、肝俞、脾俞)交替行隔药饼灸干预,每穴灸4壮,每日1次,干预12周。HE染色观察主动脉组织病理变化,比色法检测总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density liporotein cholesterol,LDL-C)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)含量,ELISA法检测Ox-LDL、IFN-γ含量。结果 与正常组比,模型组主动脉内皮明显增厚,平滑肌排列絮乱,泡沫细胞大量聚集,血清TC、TG、LDL-C、Ox-LDL、IFN-γ含量均显著升高(P<0.05,P<0.001),HDL-C含量显著下降(P<0.001)。与模型组比,隔药饼灸组主动脉内皮结构完整,平滑肌排列整齐,少见泡沫细胞,血清TC、TG、LDL-C、ox-LDL、IFN-γ含量均显著下降(P<0.05,P<0.01,P<0.001),HDL-C含量明显升高(P<0.05)。结论 隔药饼灸可能通过降低AS兔血清Ox-LDL、IFN-γ含量,减少巨噬细胞脂质摄取,抑制泡沫细胞形成,发挥抗AS的作用。 展开更多
关键词 动脉粥样硬化 隔药饼灸 氧化低密度脂蛋白 干扰素-γ 泡沫细胞 脂质
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血清LDL-C/HDL-C、non-HDL-C、RLP-C及sLOX-1水平与老年急性脑梗死的相关性研究 被引量:1
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作者 张萌 刘海颖 齐畅 《临床误诊误治》 CAS 2024年第1期83-88,共6页
目的分析血清低密度脂蛋白胆固醇(LDL-C)/高密度脂蛋白胆固醇(HDL-C)、非高密度脂蛋白胆固醇(non-HDL-C)、残粒脂蛋白胆固醇(RLP-C)及可溶性凝集素样氧化型低密度脂蛋白受体-1(sLOX-1)水平与老年急性脑梗死(ACI)的相关性。方法选取2022... 目的分析血清低密度脂蛋白胆固醇(LDL-C)/高密度脂蛋白胆固醇(HDL-C)、非高密度脂蛋白胆固醇(non-HDL-C)、残粒脂蛋白胆固醇(RLP-C)及可溶性凝集素样氧化型低密度脂蛋白受体-1(sLOX-1)水平与老年急性脑梗死(ACI)的相关性。方法选取2022年1月—2023年8月收治的230例老年ACI作为研究组,另选择同期、同年龄段115例健康体检者作为对照组。比较2组LDL-C/HDL-C、non-HDL-C、RLP-C及sLOX-1水平,比较研究组不同斑块稳定性、神经功能缺损程度[采用美国国立卫生研究院卒中量表(NIHSS)评分评估]、颈动脉内中膜厚度(IMT)患者上述指标水平,偏回归分析上述指标与老年ACI的关系,并分析上述指标与斑块稳定性、IMT、NIHSS评分的相关性。结果研究组LDL-C/HDL-C、non-HDL-C、RLP-C、sLOX-1水平高于对照组(P<0.01)。老年ACI患者LDL-C/HDL-C、non-HDL-C、RLP-C及sLOX-1水平,无斑块患者<稳定斑块患者<不稳定斑块患者,轻度神经功能缺损患者<中度神经功能缺损患者<重度神经功能缺损患者,IMT正常患者<IMT增厚患者<斑块形成患者(P<0.05)。LDL-C/HDL-C、non-HDL-C、RLP-C、sLOX-1水平与老年ACI密切相关(P<0.01)。研究组LDL-C/HDL-C、non-HDL-C、RLP-C及sLOX-1水平分别与斑块稳定性、IMT、NIHSS评分呈正相关(P<0.01)。结论老年ACI患者LDL-C/HDL-C、non-HDL-C、RLP-C、sLOX-1水平明显升高,且与神经功能缺损程度及颈动脉粥样硬化斑块密切相关。 展开更多
关键词 急性脑梗死 低密度脂蛋白胆固醇 高密度脂蛋白胆固醇 非高密度脂蛋白胆固醇 残粒脂蛋白胆固醇 可溶性凝集素样氧化低密度脂蛋白受体-1 老年人 相关性
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丹皮酚通过调控动脉粥样硬化中的miR-152-3p/ASPH轴抑制氧化低密度脂蛋白诱发的小鼠血管内皮细胞凋亡、炎症反应和氧化应激
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作者 刘岩 张磊 +2 位作者 安硕 史玉娟 秦素霞 《沈阳药科大学学报》 CAS CSCD 2024年第9期1244-1253,共10页
目的揭示丹皮酚通过调控miR-152-3p/ASPH轴在ox-LDL诱导的血管内皮细胞(vascular endothelial cells,VECs)进展中的作用。方法首先,用不同浓度的氧化低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)(0、25、50、100μg·mL^... 目的揭示丹皮酚通过调控miR-152-3p/ASPH轴在ox-LDL诱导的血管内皮细胞(vascular endothelial cells,VECs)进展中的作用。方法首先,用不同浓度的氧化低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)(0、25、50、100μg·mL^(-1))处理小鼠VECs,选取100μg·mL^(-1)ox-LDL进行实验。然后,用不同浓度的丹皮酚(30、60、120μmol·L^(-1))或辛伐他汀(30μmol·L^(-1))处理VECs。接着,使用细胞计数试剂盒-8和流式细胞术分别用于测定细胞活力和凋亡。此外,使用酶联免疫吸附法分析了IL-1β和IL-6的水平,并使用相关试剂盒检测了活性氧、乳酸脱氢酶和丙二醛的含量。此外,通过qRT-PCR或Western blot检测miR-152-3p和天冬氨酰(天冬酰胺)β-羟化酶(aspartate-β-hydroxylase,ASPH)的表达。miR-152-3p和ASPH之间的相互作用由starBase预测,然后通过双荧光素酶报告基因实验和RNA结合蛋白免疫沉淀实验证实。结果100μg·mL^(-1)ox-LDL显著抑制VECs活力,并促进细胞凋亡、炎症反应和氧化损伤。而丹皮酚的处理以剂量依赖性的方式增加细胞活力,抑制ox-LDL诱导的细胞凋亡。丹皮酚以剂量依赖性的方式降低ox-LDL诱导的IL-1β和IL-6的水平,以及以剂量依赖性的方式减弱ox-LDL对ROS、MDA和LDH含量的促进作用。另外,ox-LDL对miR-152-3p表达的抑制作用和对ASPH表达的促进作用也被丹皮酚逆转,且120μmol·L^(-1)丹皮酚效果最好。120μmol·L^(-1)丹皮酚能够上调miR-152-3p或下调ASPH,进一步促进丹皮酚对VECs生长的保护作用,miR-152-3p靶向负调控ASPH表达。结论丹皮酚通过调节miR-152-3p/ASPH轴减弱ox-LDL对小鼠VECs生长的影响,为AS的治疗提供理论基础。 展开更多
关键词 丹皮酚 氧化低密度脂蛋白 miR-152-3p 天冬氨酰(天冬酰胺)β-羟化酶 血管内皮细胞 动脉粥样硬化
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oxLDL/β2GPⅠ/aβ2GPⅠ复合物通过TLR4/MyD88/NF-κB途径促进血管内皮细胞血管生成
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作者 张贵婷 何超 《天津医药》 CAS 2024年第11期1131-1136,共6页
目的探讨氧化型低密度脂蛋白(oxLDL)/β2糖蛋白Ⅰ(β2GPⅠ)/抗β2糖蛋白Ⅰ抗体(aβ2GPⅠ)复合物对血管内皮细胞增殖、迁移及血管生成的影响及其机制。方法将人脐静脉内皮细胞(HUVEC)培养至对数生长期,分为对照组(正常培养)、oxLDL组(50 ... 目的探讨氧化型低密度脂蛋白(oxLDL)/β2糖蛋白Ⅰ(β2GPⅠ)/抗β2糖蛋白Ⅰ抗体(aβ2GPⅠ)复合物对血管内皮细胞增殖、迁移及血管生成的影响及其机制。方法将人脐静脉内皮细胞(HUVEC)培养至对数生长期,分为对照组(正常培养)、oxLDL组(50 mg/L oxLDL)、oxLDL/β2GPⅠ/aβ2GPⅠ组(50 mg/L oxLDL/100 mg/Lβ2GPⅠ/100 mg/L aβ2GPⅠ)和血管内皮生长因子(VEGF)组(100μg/L VEGF)。实时荧光定量PCR(qPCR)法检测血管生成相关因子VEGF、血管内皮钙黏蛋白(VE-cadherin)、基质金属蛋白酶(MMP)-2及MMP-9的基因表达;CCK-8检测细胞增殖;划痕愈合实验和Transwell实验检测细胞的迁移和侵袭能力;基质胶管腔形成实验检测HUVEC的血管生成能力;Western blot法检测Toll样受体4(TLR4)、髓样分化因子88(MyD88)和核因子κB(NF-κB)的蛋白表达。结果与对照组相比,oxLDL/β2GPⅠ/aβ2GPⅠ组细胞增殖活力在处理48 h时增加;此外,与对照组相比,oxLDL/β2GPⅠ/aβ2GPⅠ组细胞迁移及血管生成能力增强,VEGF、VE-cadherin、MMP-2及MMP-9的mRNA水平升高(P<0.05),TLR4和MyD88蛋白水平及p-NF-κB p65/NF-κB p65比值升高(P<0.05)。结论oxLDL/β2GPⅠ/aβ2GPⅠ复合物可能通过TLR4/MyD88/NF-κB通路诱导血管内皮细胞增殖、迁移及血管生成。 展开更多
关键词 动脉粥样硬化 β2糖蛋白Ⅰ 新生血管化 病理性 细胞增殖 细胞运动 NF-ΚB 内皮细胞 氧化型低密度脂蛋白
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