Effect of Acute Low Temperature Stress on Oxygen Consumption Rate and Respiration Frequency in Silurus meridionalis and Pelteobag vachelli

The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investigation was operated under the temperature of 24 ℃.It was found that the VO2 and Vf of Silurus meridionalis after 6 and 0 ℃ stress showed a decrease-increase-decrease trend while other groups showed a rapid increase then slowly recovery trend.The VO2 and Vf of Pelteobag vachelli after 0 ℃stress showed a decrease-increase-decrease trend while other groups showed an increase then slowly recovery process.It was suggested that Pelteobag vachelli was more adaptive to acute cold stress,but it cost more energy adapting to cold stress compared to Silurus meridionalis.

A Study of Real-time Peak Oxygen Consumption and Six-minute Walk Test

Objectives To assess the relationship between peak oxygen consumption （PVO2） and the ambulation distance in six-minute walk test （6MWT）among the healthy subjects. Methods The 51 healthy subjects were recruited for the six-minute walk test. Data of pulmonary gas exchange breath by breath, such as VO2 , VCO2 were real-time measured with wireless remote sensing K4B2, so to study the relationship between peak oxygen uptake and the ambulation distance. Results It was noticed that there was a positive linear correlation between the ambulation distance and PVO2 （ r =0. 619, P 〈0. 001 ） in six-minute walk test. The regression equation was set up （ VO2/kg =0. 05D -6. 331, P 〈 0. 001 ）. PVO2 〉 PVCO2 ,R 〈 1 were found,which suggested that 6MWT was a test below anaerobic threshold. Conclusions There was a closely positive linear correlation between the ambulation distance and PVO2, which is safety, convenient and valuable for the evaluation of cardiopulmonary function and the treatment of cardiopulmonary rehabilitation.

Effects of various anesthetic techniques and PaCO_2 levels on cerebral oxygen balance in neurosurgical patients

Objective: To assess the effects of various anesthetic techniques and PaCO2 levels on cerebral oxygen supply/consumption balance during craniotomy for removal of tumors, and to explore an anesthetic technique for neurosur-gery and an appropriate degree of PaCO2 during neuroanesthesia. Methods: One hundred and fourteen patients with supratentorial tumors for elective craniotomy, ASA grade I - II , were randomly allocated to six groups. Patients were anesthetized with continuous intravenous infusion of 2% procaine 1. 0 mg · kg-1 · min-1 in Group I , inhalation of 1. 0% - 1. 5% isoflurane in Group II , and infusion of 2% procaine 0. 5 mg·kg · min-1 combined with inhalation of 0.5% -0.7% isoflurane in Group III during the period of study. The end-tidal pressure of CO2(PET CO2 ) was maintained at 4.0 kPa in these 3 groups. In Group IV, V and VI, the anesthetic technique was the same as that in Group I but the PETCO2 was adjusted to 3. 5, 4. 0 and 4. 5 kPa respectively for 60 min during which the study was performed. The radial arterial and retrograde jugular venous blood samples were obtained at the onset and the end of this study for determining jugular venous bulb oxygen saturation ( SjvO2 ) , arteriovenous oxygen content difference (AVDO2) and cerebral extraction of oxygen (CEO2). Results: In Group I and I SjvO2, AVDO2 and CEO2 remained stable. Although SjvO2 kept constant, AVDO2 and CEO2 decreased significantly (P <0. 05) in Group II. Moreover, AVDO2 and CEO2 in Group II were significantly lower than those of Group III (P<0. 05). In Group IV, 60 min after hyperventilation, SjvO2 and jugular venous oxygen content ( CjvO2 ) decreased markedly (P < 0. 01 ) while CEO2 increased significantly ( P <0.01) . In addition, SjvO2, CjvO2 and CEO2 in Group IV were significantly different from the corresponding parameters in Group V and Group VI (P <0. 05) . In view of sustained excessive hyperventilation, SjvO2 was less than 50% in 37.5% patients of Group IV. Conclusion: Anesthesia with intravenous infusion of procaine combined with isoflurane inhalation proved to be more suitable for neurosurgery than procaine intravenous anesthesia or isoflurane inhalation anesthesia alone. PaCO2 at 4.0 -4. 5 kPa in patients undergoing craniocerebral surgery during neuroanesthesia would be beneficial in both decreasing ICP and maintaining cerebral oxygen supply/consumption balance.

Zn2＋对日本沼虾(Macrobrachium nipponensis)的急性致毒效应

在水温(27.4±1.3)℃条件下,以体长(33.23±1.66)mm、体质量(0.7893±0.1518)g的日本沼虾为实验动物,采用静水停食法开展了Zn2+对日本沼虾的急性毒性实验,并以此为基础,测定了Zn2+不同质量浓度水平下日本沼虾的耗氧率与窒息点。结果表明:(1)Zn2+对日本沼虾的急性致死效应与其呼吸生理代谢受阻密切相关;(2)Zn2+对日本沼虾24h、48h、72h、96h的半致死质量浓度依次为1.493、0.844、0.643和0.464mg/L;(3)Zn2+对日本沼虾昼均、夜均和日均耗氧率均无显著影响的阈值为0.046mg/L,该值为Zn2+对日本沼虾急性毒性的安全质量浓度;(4)Zn2+对日本沼虾产生轻度胁迫和中度胁迫的质量浓度范围分别为0.046—0.064mg/L和0.064—0.082mg/L。

盐度和Ca^(2+)浓度对中国明对虾稚虾耗氧率的影响

设计双因子实验研究了水温25.0℃±0.5℃下,盐度(5,15,30)和Ca2+质量浓度(175,350,700,1 400,2 800 mg/L)对中国明对虾(Fenneropenaeus chinensis)稚虾耗氧率的影响,实验对虾的湿体质量为0.301 g±0.041 g。实验结果表明:(1)不同盐度下,中国明对虾稚虾耗氧率的大小顺序为R5>R30>R15。其中,盐度15下对虾的耗氧率显著低于盐度5和30下的耗氧率(P<0.05);(2)不同Ca2+质量浓度下,中国明对虾稚虾耗氧率的大小顺序为R2 800>R175>R700>R1 400>R350。其中,Ca2+质量浓度为350 mg/L组对虾的耗氧率显著低于其它处理组,Ca2+质量浓度为2 800 mg/L组对虾的耗氧率显著高于其它处理组(P<0.05),而Ca2+质量浓度为175,700和1 400 mg/L组间对虾的耗氧率差异不显著(P>0.05);(3)盐度和Ca2+质量浓度的交互作用显著影响中国明对虾稚虾的耗氧率(P<0.05)。

个体化有氧联合抗阻运动对冠心病并发2型糖尿病患者血糖、血脂以及运动能力的影响

目的探讨个体化有氧联合抗阻运动对冠状动脉粥样硬化性心脏病(冠心病)并发2型糖尿病(type 2diabetes mellitus,T2DM)患者血糖、血脂等生理指标以及运动能力的影响,并分析其可能机制。方法招募冠心病并发T2DM患者60例纳入研究,并按随机数字分组法随机分为康复组(30例)和对照组(30例)。对照组进行常规药物治疗;康复组在对照组基础上加以根据心肺运动试验(cardiopulmonary exercise test,CPET)测试评估结果,进行强度为40%~60%最大摄氧量(maximal oxygen consumption,VO_2max)的有氧运动,抗阻训练以弹力带测定10 RM为强度。每次联合运动时间30~60 min,每周3次,共12周。12周的治疗前后所有患者测定空腹血糖(FPG)、餐后2 h血糖(PBG)、糖化血红蛋白(Hb A1c)、总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、脂肪重(FM)、瘦体质量(LBM)和VO_2max。结果对照组治疗前、后各项指标比较,差异无统计学意义(P>0.05)。康复组的血糖、血脂以及运动能力治疗前、后比较,差异有统计学意义(P<0.05)。治疗后康复组各项观察指标明显优于对照组,差异具有统计学意义(P<0.05)。结论个体化有氧联合抗阻运动能够改善冠心病并发T2DM患者血糖、血脂浓度以及运动能力。

应用DATEXUltima监测仪观察CO_2气腹对机体呼吸功能和氧耗的影响

目的 :通过 Ultima监测仪观察 CO2 气腹对机体呼吸和氧耗有何影响。方法 :在气管内静吸全麻复合硬膜外麻醉下行 L C。以 Ultima监测仪持续监测呼吸功能和气体的变化。结果 :PETCO2 和 VCO2 在气腹 2 0 m in后较气腹前显著增加。 PAW在气腹开始后较气腹前显著升高 (P<0 .0 0 1)。 MVE在气腹后较气腹前降低 (P>0 .0 5 )。 VO2 在气腹后 10min时显著高于气腹前水平 (P<0 .0 1) ,以后继续升高。结论 :在 CO2 气腹期间 ,通过 Ultima监测仪能连续观察 PETCO2 、VT、MVE、PAW及 VO2 的动态变化。

富氧焙烧技术在109 m^(2)锌精矿流态化焙烧炉上的应用实践

多年来,国内富氧焙烧技术在锌精矿焙烧炉上的应用始终停留在试验阶段,驰宏会冶首次实现了锌精矿富氧焙烧规模化生产。该公司通过在原鼓风系统管道上增加富氧分布装置,配套有高浓度氧气调节阀组及流量、压力监测仪表等设施,同时,配套改进流态化冷却器、高温引风机和鼓风机等设备,实现了富氧焙烧技术在锌精矿焙烧炉上的应用。生产实践表明,在适当的工艺参数下,富氧焙烧技术可以充分释放产能,流态化焙烧炉床能率提升到8.03 t/m^(2)·d;焙烧矿质量得到有效改善,烟尘含硫下降至3.5%;生产吨锌能耗大幅降低,空压风单耗194.0 Nm^(3)/t,电单耗135.80 kW·h/t,高浓度氧气的单耗为81 Nm^(3)/t。综上,109 m^(2)焙烧炉采用富氧焙烧技术后获得了较好的经济效益,另外,还提高了焙烧系统、硫酸系统装置的利用率,降低了鼓风量,有效防止了烟尘的无组织排放,改善了现场生产环境。

盐度和亚硝酸盐氮胁迫对菲律宾蛤仔“斑马蛤2号”和莆田群体代谢的影响

为研究低盐度和亚硝酸盐氮协同对菲律宾蛤仔(Ruditapes philippinarum)的胁迫作用,选取菲律宾蛤仔“斑马蛤2号”(壳长为27.5 mm±1.2 mm)和莆田群体(壳长为31.2 mm±1.8 mm)为试验对象,以盐度30的天然海水为对照,设定盐度分别为25、20、15,亚硝酸盐氮质量浓度分别为0.15、0.30、0.60 mg/L,通过分析菲律宾蛤仔耗氧率、排氨率和鳃丝结构的变化,探讨了低盐度和亚硝酸盐氮单因子胁迫及二者协同胁迫对菲律宾蛤仔呼吸代谢的影响。结果表明:在低盐度胁迫下,“斑马蛤2号”和莆田群体的耗氧率均随盐度的降低而增加,排氨率均呈先降低后增加的趋势;在亚硝酸盐氮胁迫下,“斑马蛤2号”和莆田群体的耗氧率均随亚硝酸盐氮质量浓度的增加而增加,“斑马蛤2号”的排氨率呈先降低后增加的趋势,而莆田群体的排氨率则呈先增加后降低的趋势;在低盐度和亚硝酸盐氮协同作用下,“斑马蛤2号”和莆田群体的耗氧率和排氨率均较低盐度和亚硝酸盐氮单因子胁迫时明显增加,且菲律宾蛤仔鳃组织结构受损,鳃丝出现呼吸上皮细胞脱落;对菲律宾蛤仔代谢过程的氧氮比(O∶N)分析显示,其代谢主要由蛋白质氧化供能,但在低盐度和亚硝酸盐氮协同胁迫下主要由蛋白质供能,辅以脂肪和碳水化合物供能。研究表明,低盐度和亚硝酸盐氮协同胁迫,影响菲律宾蛤仔鳃组织的正常生理功能,并导致其呼吸代谢机能减弱。

不同预氧化程度焦煤CO_(2)冷却后自燃特性研究

针对利用惰性气体降低煤氧化性来解决煤自燃、复燃的问题,现有研究大多是对煤低温氧化过程及煤复燃过程进行相关实验,对惰性气体降温后煤二次氧化的自燃特性涉及较少。针对上述问题,以焦煤为例,通过低温氧化实验,探究不同温度氧化的焦煤经过CO_(2)冷却二次氧化的自燃特性。采用GC-4000A程序升温装置对焦煤进行预氧化(预氧化温度分别设为70,110,150℃),并对分别通入CO_(2)气体和干空气冷却至30℃后焦煤二次氧化过程中的耗氧速率、CO产生率、CO_(2)浓度和表观活化能进行分析。实验结果表明:预氧化温度相同时,与干空气冷却相比,通入CO_(2)冷却后的焦煤相关参数的变化规律基本一致,二次氧化初期,因预氧化焦煤吸附大量CO_(2),阻碍了煤与O_(2)接触,耗氧速率和CO产生率减小,表观活化能增大,焦煤的氧化性减弱;随着CO_(2)解析,CO_(2)冷却也影响预氧化焦煤的后期反应,使得预氧化焦煤整个反应过程自燃危险性降低。预氧化温度不同时,70℃和110℃预氧化焦煤前期CO_(2)吸附量小,导致耗氧速率、CO产生率和表观活化能未发生变化,150℃预氧化焦煤冷却至30℃时,CO_(2)吸附量增多,导致耗氧速率、CO产生率减小,表观活化能增大,需要的能量更多,煤氧反应更难进行,自燃危险性有所降低。因此,当煤矿井下发生煤氧化自燃危险时,需长时间通入CO_(2)来降低矿区启封复采时发生二次氧化复燃的可能性。

太平洋牡蛎在对Pb^(2+)富集和排出过程中的生理水平变化

为研究牡蛎对Pb^(2+)富集和排出过程中生理代谢的变化,将太平洋牡蛎(Crassostrea gigas)暴露于含有浓度为0.2 mg/L Pb^(2+)的海水中,18 d后转入洁净海水中继续暂养18 d。实验结果表明,在牡蛎对Pb^(2+)富集的过程中,其摄食率、滤水率、耗氧率和排氨率显著提高,转入洁净海水培养后,其摄食率、滤水率、耗氧率和排氨率迅速降低,6 d左右恢复正常水平。总体来说,在浓度为0.2 mg/L Pb^(2+)海水胁迫下,牡蛎通过提升其生理代谢水平以抵御Pb^(2+)的毒性影响,转入洁净海水后,Pb^(2+)对牡蛎生理代谢产生的毒性影响迅速恢复。

中华绒螯蟹幼蟹标准代谢的研究

对中华绒螯蟹幼蟹的耗氧率、CO2 排出率及NH3-N排泄率进行了测定 ,并研究了幼蟹的能耗率及能源物质的供能比。结果表明 ,体重 ( 2 70± 1 4 0 )g的幼蟹 ,在水温 ( 2 0± 0 5 )℃时 ,耗氧率、CO2 排出率和NH3-N排泄率分别为 ( 0 4 14± 0 0 91)mg·g-1·h-1、( 0 4 76± 0 12 5 )mg·g-1·h-1和 ( 4 179± 1 171) μg·g-1·h-1;标准代谢的蛋白质、碳水化合物和脂肪提供的能量比为 7 3∶3 8 8∶5 3 9。其耗氧率 (R0 )受水中溶氧水平的影响 ,并与体重(W )呈负相关 (R0 =0 84 0 3W-0 72 6 5)。

糖尿病脂肪肝患者血浆Vaspin与代谢指标的相关性研究

目的:研究2型糖尿病(T2DM)合并非酒精性脂肪性肝病(NAFLD)患者血浆丝氨酸蛋白酶抑制剂(vaspin)水平与代谢指标的关系。方法:采用酶联免疫吸附法(ELISA)检测T2DM合并NAFLD组(A组)、T2DM不合并NAFLD组(B组)和正常对照(NC)组的血浆vaspin水平,采用间接测热法检测能量代谢指标,同时测定相关的临床指标。结果:B组血浆vaspin水平高于A组和NC组,A组和B组呼吸高(RQ)值和碳水化合物氧化率显著低于NC组,而24h尿素氮、静息代谢率(REE)、REE/体质量和脂肪氧化率显著高于NC组(P<0.05或P<0.01),A、B组间差异均无统计学意义(P>0.05)。A组血浆vaspin水平与体质量、体质量指数(BMI)、REE和性别(男=23,女=20)呈正相关,与RQ呈负相关;B组血浆vaspin水平与BMI、腰臀比(WHR)、空腹血糖(FBG)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、REE、REE/体质量、TG呈正相关,与RQ呈负相关。vaspin值为92.4μg/L时可作为预测2型糖尿病患者脂肪肝的风险值。结论:血浆vaspin水平与物质及能量代谢密切相关,并可用来预测2型糖尿病患者脂肪肝的风险。

Elevated metabolic rate during passive stretching is not a sufficient aerobic warm-up

Purpose： The purpose of this study was to determine the extent that a static stretching program could increase heart rate （HR） and oxygen consumption （VO2）, and if the increases were sufficient to serve as a warm-up for aerobic activity. Methods： The HR and VO2 of 15 male and 16 female college students were measured after either 12 min of passive static stretching （SS）, or 12 rain pseudo-stretching （PS）, which consisted of moving through the stretching positions without placing the muscles on stretch. Four different lower body stretches were used with each stretch held for 30 s before the participant moved to a different position, with the circuit being repeated four times. VO2 was determined by averaging breath-by-breath measures over the total 12 min. HR was obtained every 30 s and the 24 values were averaged. Warm-up benefit was determined from the 02 deficit accrued during 7-min cycling at 60% VOzmax. Results： HR （beats/rain, mean 4. SD） for SS （84 ± 11） was a significant （p 〈 0.05） 9% greater than PS （78 ± 12）. Similarly, VO2 （mL/min, mean 4. SD） for SS （0.53 ± 0.13） was a significant 44% greater than PS （0.38 ± 0.11）. The O2 deficit （L, mean 4, SD） for SS （0.64 ± 1.54） was not different from PS （0.72 ± 1.61）. Conclusion： These data indicate that passive static stretching increases both HR and VO2, indicating that metabolic activity can be increased without muscle activation. The magnitude of the increases, however, is not sufficient to elicit a warm-up effect.

Synthesis of cobalt vanadium nanomaterials for efficient electrocatalysis of oxygen evolution

A low-cost and high-activity catalyst for oxygen evolution reaction （OER） is the key to the water splitting technology for hydrogen generation. Here we report the use of three solvents, DMF, ethanol and glycol, in the solvothermal synthesis of three nano-catalysts, Co3（VO4）2-I, Co3（VO4）2-II, and Co3（VO4）2-III, respec- tively. Transmission electron microscope shows Co3（VO4）2-I, II, and III exist as ultrafine nanosheets, ultrathin nanofilms, and ultrafine nanosheet-comprised microspheres, respectively. These Co3（VO4）2 catalysts exhibit OER electrocatalysis, among which the Co3（VO4）2-II shows the lowest onset overpotential of 310 mV and only requires a small overpotential of 330 mV to drive current density of 10 mA/cm^2. Due to their high surface free energy, the ultmthin nanofilms of Co3（VO4）2- II exhibits a good immobilization effect with the high electrocatalytic activity for OER.

Over-ground walking in Parkinson’s disease: A pilot study utilizing a portable metabolic analyzer

Alterations in gait biomechanics are common during early stages of Parkinson’s disease (PD), potentially elevating energy requirements of walking and leading to impaired economy of gait. Although gait economy is traditionally assessed during treadmill walking with simultaneous ox-ygen consumption (VO2) monitoring, treadmill gait mechanics, particularly in PD, may be different from over-ground walking mechanics, possibly providing a distorted picture of true gait economy. Currently, no studies have directly examined the energy cost of over-ground walking in PD patients. The purpose of this study was to test the feasibility of measuring energy expenditure during over-ground walking in mild to moderate PD using portable gas exchange monitoring technology. Additionally, we sought to determine whether energy expenditure, as assessed through VO2 measures, related to disease severity for PD. Seventeen PD patients underwent separate 6-minute walk (6MW) tests both with and without the COSMED K4b2 portable oxygen monitoring system. Gait economy was calculated as measured VO2 during 6MW divided by the predicted VO2 for non-PD age-matched subjects, according to a standard estimation equation utilizing ground speed. Distance covered during the 6MW with the portable system (420 ± 12 meters) was highly correlated (r = 0.96, p 2 peak for normal floor walking, and show impaired gait economy relative to prediction equations. Interestingly, the degree of elevated energy expenditure during gait did not relate to disease severity.

Oxygen vacancy self-doped single crystal-like TiO_(2) nanotube arrays for efficient light-driven methane non-oxidative coupling

Photocatalytic non-oxidative coupling of methane(PNOCM)is a mild and cost-effective method for the production of multicarbon compounds.However,the separation of photogenerated charges and activation of methane(CH4)are the main challenges for this reaction.Here,single crystal-like TiO_(2) nanotubes(VO-p-TNTs)with oxygen vacancies(VO)and preferential orientation were prepared and applied to PNOCM.The results demonstrate that the significantly enhanced photocatalytic performance is mainly related to the strong synergistic effect between preferential orientation and VO.The preferential orientation of VO-p-TNT along the[001]direction reduces the formation of complex centers at grain boundaries as the form of interfacial states and potential barriers,which improves the separation and transport of photogenerated carriers.Meanwhile,VO provides abundant coordination unsaturated sites for CH4 chemisorption and also acts as electron traps to hinder the recombination of electrons and holes,establishing an effective electron transfer channel between the adsorbed CH4 molecule and photocatalyst,thus weakening the C–H bond.In addition,the introduction of VO broadens the light absorption range.As a result,VO-p-TNT exhibits excellent PNOCM performance and provides new insights into catalyst design for CH4 conversion.

Impact of Novel Pulsed Electromagnetic Field Device on Competitive Athlete Performance

The interaction of pulsed electromagnetic fields (PEMF) with the human body may result in a variety of positive outcomes including analgesia, enhanced healing, chondroprotection, cognitive improvement and better quality of life. Previous human studies have also revealed the potential of PEMF to enhance muscle function and athletic performance. To further evaluate this potential, an open label pilot study was conducted with 19 competitive cyclists who repeatedly participated in 63 training routes. Cyclist performance was tracked before and during use of a novel and portable PEMF device that is worn as a wristband. Comparison of performance before and during use of the wristband revealed a significant association with improved muscle power. The odds ratio was 3.02 (P < 0.01) for experiencing increased muscle power while wearing the PEMF device. Among the cycling routes in which an increase was observed, the average increase in power was about 9.8%. The data suggests the novel PEMF technology may be a safe and effective therapeutic approach for improved physical performance and likely involves improved oxygen delivery due to reduced rouleaux (erythrocyte aggregation). These results warrant further investigation comprising larger studies and additional outcomes.

Perceived Improvements of Quality of Life (QoL) among Patients with Idiopathic Pulmonary Fibrosis (IPF) in Response to a 6-Week Rehabilitation Program

Idiopathic pulmonary fibrosis (IPF) is a chronic, life-limiting with an average life expectancy of 05 years following the onset of the disease, with no curative treatments. These patients need palliative care and rehabilitation is one of the methods that can be used to improve quality of life (QoL) among these patients. Yet the research conducted to assess benefits of pulmonary rehabilitation (PR) in terms of improving physical activity and QoL in IPF patients remains limited. Hence this study aims to evaluate the effect of a bespoke pulmonary rehabilitation programme, on the physical, physiological and psychological parameters and improvements of QoL among IPF patients. Eleven (11) subjects with IPF received 6 weeks of pulmonary rehabilitation. An interviewer administered quality of life questionnaire, six-minute walking test (6MWT), Incremental bicycle exercise tests were performed, and cardiac and respiratory parameters were assessed pre- and post-rehabilitation. The 6MWT was significantly increased following training (Pre 312.55 ± 89.99;Post, 380.73 ± 59.60). A significant improvement was observed in overall QoL (2.226 ± 0.026), dyspnoea (-0.455 ± 0.004) anxiety (-2.070 ± 0.038), depression (-2.217 ± 0.027) scores. No significant changes were found in the VO2 max and other cardiopulmonary parameters, while non-significant improvement was seen in SpO2 at peak exercise from 85.8 - 86.5. Bespoke pulmonary rehabilitation program is beneficial in short term improvement of the functional exercise capacity, dyspnoea and QoL among IPF patients.

Mitochondria as the main source of vanadyl acetylacetonate-induced reactive oxygen species generation in renal epithelial cell lines

In this study, we aimed to clarify the source of the reactive oxygen species （ROS） generation induced by vanadium compounds. We used vanadyl acetylacetonate （VO（acac）2）, a highly effective agent in controlling hyperglycemia, to determine the source of ROS generation in two renal cell lines LLC-PK1 and MDCK. Four commonly fluorescent dyes were used to assess VO（acac）2-induced H202 and ＂02 production and their location. It demonstrated that VO（acac）2 can induce significant ROS generation in both LLC-PKI and MDCK cells, which were primarily derived from mitochondria. The results obtained in this study raised the possibility to reduce ROS level induced by vanadium compounds locally and thus avoid affecting its activity.