8,4′-Oxyneolignane glucosides from an aqueous extract of“ban lan gen”(Isatis indigotica root)and their absolute configurations

Three pairs of glycosidic 8,4′-oxyneolignane diastereoisomers, named isatioxyneolignosides A-F(1–6), were isolated from an aqueous extract of Isatis indigotica roots. Their structures and absolute configurations were elucidated by comprehensive spectroscopic data analysis and enzyme hydrolysis. The validity of Δδ_(C8-C7) values to distinguish threo and erythro aryl glycerol units and Cotton effects at 235±5 nm to determine absolute configurations at C-8 in 1–6 and their aglycones(1a–6a) are discussed.

A novel 8,4'-oxyneolignan diglycoside from Ligusticum sinensis

A novel 8,4＇-oxyneolignan diglycoside,named ligusinenoside d（1）,was isolated from the rhizomes of Ligusticum sinensis, together with five known analogues 2-6.The absolute configurations of 1 and 2 were elucidated by means of enzymatic hydrolysis and spectroscopic data.

黄皮根和茎中一个新的木脂体(英文)

从黄皮(Clausena lansium)根和茎中分离得到8个化合物,其中化合物claulignan(1)为一个新的木脂体类化合物,通过1D-、2D-NMR和高分辨质谱确定其结构。化合物2-8首次从黄皮属(Clausena)植物中分离得到。活性筛选结果表明,化合物1和6显示一定的细胞毒活性,对人宫颈癌HeLa细胞株的IC50分别为25.03和53.99μM;化合物3具有一定的抗氧化活性,在DPPH实验中的EC50为268.96g·kg-1。

Lignans with NO inhibitory activity from Tinospora sinensis

Two new lignan glucosides,tinsinlignans A and B(1 and 2),two new oxyneolignans,tinsinlignans C and D(3 and 4),along with one known analogue(5),were isolated from the stems of Tinospora sinensis.The structures of the new compounds were elucidated based on analysis of spectroscopic data,and the absolute configuration of 1 was determined through electronic circular dichroism(ECD)calculation based on the time-dependent density functional theory(TD-DFT).Compounds 1−4 were evaluated for their inhibitory effects on nitric oxide(NO)production induced by lipopolysaccharide(LPS)in murine RAW264.7 macrophage cells and compounds 1 and 2 exhibited moderate inhibitory activities with IC50 values of 18.5±2.0 and 28.8±1.2μmol·L^(−1),respectively.

仙鹤草中木脂素类化学成分的研究

目的对仙鹤草Agrimonia pilosa的化学成分进行分离和鉴定,并利用去血清损伤的PC12细胞模型筛选化合物的神经保护活性。方法利用80%乙醇对仙鹤草进行提取,再通过大孔吸附树脂、ODS、硅胶、Sephadex LH-20凝胶以及半制备型高效液相等柱色谱方法进行分离,然后根据质谱、核磁、圆二色谱等波谱学数据鉴定化合物的结构。结果从仙鹤草中分离并鉴定了10个木脂素类化合物,分别命名为仙鹤草酸酯(1)、仙鹤草苷A(2)、苦树苷C(3)、长花马先蒿苷B(4)、(7R,8S)-4,7,9,9′-四羟基-3,3′-二甲氧基-8-O-4′-新木脂素-7-O-β-D-葡萄糖苷(5)、(7S,8S)-3-甲氧基-8,4′-氧代新木脂素-3′,4,7,9,9′-五醇(6)、红叶藤苷(7)、(7S,8R)-1-[4-O-(β-D-吡喃葡糖糖基)-3-甲氧基苯基]-2-[4-(3-羟丙基)-2,6-二甲氧基苯氧基]-1,3-丙二醇(8)、密穗马先蒿苷(9)、淫羊藿醇A2(10)。结论化合物1和2为新化合物,其余化合物均为首次从仙鹤草中分离得到。化合物1a和1b为1对互变的葡萄糖端基手性相反的8-O-4′型木脂素酸酯;化合物1在10μmol/L对去血清损伤的PC12神经细胞表现出一定的保护作用。