Anti-lipid peroxidation activity, reducing power and chelating power of flavonoid extracts from Oxytropis glabra DC, as well as their elimination effect on DPPH, superoxide anion and hydroxyl radicals were determined ...Anti-lipid peroxidation activity, reducing power and chelating power of flavonoid extracts from Oxytropis glabra DC, as well as their elimination effect on DPPH, superoxide anion and hydroxyl radicals were determined in the paper, so as to evaluate in vitro antioxidative activities of flavonoid extracted from O. glabra. The results showed that flavonoid from O. glabra had fairly strong anti-lipid peroxidation. The antioxidation effects on rapeseed oil successively were vitamin C 〉 sample 〉 vitamin E 〉 BHT 〉 CK; the effects on cottonseed oil successively were sample 〉 vitamin C 〉 BHT 〉 vitamin E 〉 CK; the effects on sunflower oil successively were sample 〉 vitamin C 〉 vitamin E 〉 BHT 〉 CK; the effects on lard successively were BHT 〉 vitamin C 〉 sample 〉 vitamin E 〉 CK. The samples had stronger clearance rate against DPPH, superoxide anion and hydroxyl radicals than control; the reducing power of sample was weaker than Vitamin C and stronger than Vitamin E ; chelating power of sample was weaker than EDTA. Flavonoid from O. glabra had the value for further development and utilization as natural antioxidant.展开更多
This study was conducted to optimize immunization program to gain the serum against swainsonine （SW） with high titer and to analyze the effects of polychinal antibody in treatment of loco disease of rabbits. Eightee...This study was conducted to optimize immunization program to gain the serum against swainsonine （SW） with high titer and to analyze the effects of polychinal antibody in treatment of loco disease of rabbits. Eighteen rabbits were randomly separated into control group, poisoning group and curing group, with six rabbits in each group. Rabbits in poisoning group and curing group were fed 10 g/（kg ~ d） powder of Oxytropis glabra DC every morning for 70 d till the rabbits in poisoning group began to die. From the 21＇t to 24＇h d of O. glabra administration, each of the rabbits in curing group was injected with 1 mL of polyclonal anti- body against SW every day. Blood samples were collected before O. glabra challenge, and then once every 7 d for the serological and immunological evaluation of serum. The resuhs showed that the serum alkaline phosphatase （AKP） activity and SW content in poisoning groups were significantly higher than those in control group on the 7th d （P 〈0.05 ） ; the serum aspartate transaminase （AST） and lactate dehydrogenase （LDH） activity in poisoning groups were significantly higher than those in control group on the 14th d （P 〈 0.05 ）, while the serum ct - mannosidase （AMA） and rate of E-rosette formation in poisoning groups were signifi- cantly lower than those in control group （ P 〈 0.05 ） ; the serum blood urea nitrogen （BUN）, creatinine （ CRE ） and glucose （ GLU ） concentration in poisoning groups were significantly higher than those in control group on the 21＇t d （P 〈 0.05 ）. By injecting serum against swainsonine, the serum AKP, LDH, AST, alanine transaminase （ALT） activity and contents of BUN, CRE, GLU, SW were reduced and serum AMA and rate of E-rosette formation were increased in curing group, by comparing with those in poisoning group. These results in this experiment indicated that polyclonal antibody against SW had good value of preventing from O. glabra DC.展开更多
In our previous paper, two new triterpenoid saponins extracted from Oxytropis ochrocephala Bunge have been reported. This note deals mainly with the structures of other two novel triterpenoid saponins Ⅱ and Ⅲ, isola...In our previous paper, two new triterpenoid saponins extracted from Oxytropis ochrocephala Bunge have been reported. This note deals mainly with the structures of other two novel triterpenoid saponins Ⅱ and Ⅲ, isolated from the aerial parts of Oxytropis glabra DC (Leguminosae), a poisonous plant to livestock, distributed展开更多
【目的】探讨家兔血清生化指标与小花棘豆中毒(Oxytropis glabra DC Poisoning,OgP)的关系,为小花棘豆中毒的诊治提供依据。【方法】以12只小花棘豆中毒家兔与10只正常家兔为研究对象,检测血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱...【目的】探讨家兔血清生化指标与小花棘豆中毒(Oxytropis glabra DC Poisoning,OgP)的关系,为小花棘豆中毒的诊治提供依据。【方法】以12只小花棘豆中毒家兔与10只正常家兔为研究对象,检测血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、α-甘露糖苷酶(AMA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性以及丙二醛(MDA)、尿素氮(BUN)、肌酐(CRE)、血糖(GLU)、甘油三酯(TG)、总胆固醇(TC)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)和极低密度脂蛋白(VLDL-C)含量。采用t检验对两组资料进行差异显著性比较后,再采用简单相关分析方法对各指标与小花棘豆中毒的关系进行分析。【结果】与正常对照相比,小花棘豆中毒家兔血清的AST、ALT、AKP、LDH、MDA、BUN、CRE、GLU、TC、GLO、LDL-C值均极显著升高(P<0.01),AMA、SOD、GSH-Px、CAT、TG和ALB极显著下降(P<0.01),HDL-C值显著下降(P<0.05),TP和VLDL-C含量差异不显著(P>0.05)。与小花棘豆中毒相关的主要因素有AMA、CAT、GLU、LDH、GSH-Px、AKP、TG和CRE,并建立回归方程:OgP=0.714-0.078AMA-0.069CAT+0.038GLU+0.047LDH+0.045AKP。【结论】血清AMA、CAT、GLU、LDH、GSH-Px、AKP、TG和CRE对家兔小花棘豆中毒的早期诊断、治疗、疗效观察和转归判断具有一定参考价值。展开更多
基金Supported by"12th Five-Year Plan" National Science and Technology Support Program (2011BAD17B05)Doctoral Special Fund of Xinjiang Production and Construction Corps (2008JC07)Open Project for Key Laboratory of Tarim Animal Husbandry Science and Technology of Xinjiang Production Construction Corps (HS201207)
文摘Anti-lipid peroxidation activity, reducing power and chelating power of flavonoid extracts from Oxytropis glabra DC, as well as their elimination effect on DPPH, superoxide anion and hydroxyl radicals were determined in the paper, so as to evaluate in vitro antioxidative activities of flavonoid extracted from O. glabra. The results showed that flavonoid from O. glabra had fairly strong anti-lipid peroxidation. The antioxidation effects on rapeseed oil successively were vitamin C 〉 sample 〉 vitamin E 〉 BHT 〉 CK; the effects on cottonseed oil successively were sample 〉 vitamin C 〉 BHT 〉 vitamin E 〉 CK; the effects on sunflower oil successively were sample 〉 vitamin C 〉 vitamin E 〉 BHT 〉 CK; the effects on lard successively were BHT 〉 vitamin C 〉 sample 〉 vitamin E 〉 CK. The samples had stronger clearance rate against DPPH, superoxide anion and hydroxyl radicals than control; the reducing power of sample was weaker than Vitamin C and stronger than Vitamin E ; chelating power of sample was weaker than EDTA. Flavonoid from O. glabra had the value for further development and utilization as natural antioxidant.
基金Supported by Doctoral Fund of Xinjiang Production and Construction Corps(2008JC07)Open Fund of Key Laboratory of Tarim Animal Husbandry Science and Technology,Xin Jiang Production & Construction Corps(HS201207)
文摘This study was conducted to optimize immunization program to gain the serum against swainsonine （SW） with high titer and to analyze the effects of polychinal antibody in treatment of loco disease of rabbits. Eighteen rabbits were randomly separated into control group, poisoning group and curing group, with six rabbits in each group. Rabbits in poisoning group and curing group were fed 10 g/（kg ~ d） powder of Oxytropis glabra DC every morning for 70 d till the rabbits in poisoning group began to die. From the 21＇t to 24＇h d of O. glabra administration, each of the rabbits in curing group was injected with 1 mL of polyclonal anti- body against SW every day. Blood samples were collected before O. glabra challenge, and then once every 7 d for the serological and immunological evaluation of serum. The resuhs showed that the serum alkaline phosphatase （AKP） activity and SW content in poisoning groups were significantly higher than those in control group on the 7th d （P 〈0.05 ） ; the serum aspartate transaminase （AST） and lactate dehydrogenase （LDH） activity in poisoning groups were significantly higher than those in control group on the 14th d （P 〈 0.05 ）, while the serum ct - mannosidase （AMA） and rate of E-rosette formation in poisoning groups were signifi- cantly lower than those in control group （ P 〈 0.05 ） ; the serum blood urea nitrogen （BUN）, creatinine （ CRE ） and glucose （ GLU ） concentration in poisoning groups were significantly higher than those in control group on the 21＇t d （P 〈 0.05 ）. By injecting serum against swainsonine, the serum AKP, LDH, AST, alanine transaminase （ALT） activity and contents of BUN, CRE, GLU, SW were reduced and serum AMA and rate of E-rosette formation were increased in curing group, by comparing with those in poisoning group. These results in this experiment indicated that polyclonal antibody against SW had good value of preventing from O. glabra DC.
文摘In our previous paper, two new triterpenoid saponins extracted from Oxytropis ochrocephala Bunge have been reported. This note deals mainly with the structures of other two novel triterpenoid saponins Ⅱ and Ⅲ, isolated from the aerial parts of Oxytropis glabra DC (Leguminosae), a poisonous plant to livestock, distributed
文摘【目的】探讨家兔血清生化指标与小花棘豆中毒(Oxytropis glabra DC Poisoning,OgP)的关系,为小花棘豆中毒的诊治提供依据。【方法】以12只小花棘豆中毒家兔与10只正常家兔为研究对象,检测血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、α-甘露糖苷酶(AMA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)活性以及丙二醛(MDA)、尿素氮(BUN)、肌酐(CRE)、血糖(GLU)、甘油三酯(TG)、总胆固醇(TC)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)和极低密度脂蛋白(VLDL-C)含量。采用t检验对两组资料进行差异显著性比较后,再采用简单相关分析方法对各指标与小花棘豆中毒的关系进行分析。【结果】与正常对照相比,小花棘豆中毒家兔血清的AST、ALT、AKP、LDH、MDA、BUN、CRE、GLU、TC、GLO、LDL-C值均极显著升高(P<0.01),AMA、SOD、GSH-Px、CAT、TG和ALB极显著下降(P<0.01),HDL-C值显著下降(P<0.05),TP和VLDL-C含量差异不显著(P>0.05)。与小花棘豆中毒相关的主要因素有AMA、CAT、GLU、LDH、GSH-Px、AKP、TG和CRE,并建立回归方程:OgP=0.714-0.078AMA-0.069CAT+0.038GLU+0.047LDH+0.045AKP。【结论】血清AMA、CAT、GLU、LDH、GSH-Px、AKP、TG和CRE对家兔小花棘豆中毒的早期诊断、治疗、疗效观察和转归判断具有一定参考价值。
