Preparation of Polyclonal Antibody Against Swainsonine and Its Effects in Treatment of Oxytropis glabrapoisoned Rabbits

This study was conducted to optimize immunization program to gain the serum against swainsonine （SW） with high titer and to analyze the effects of polychinal antibody in treatment of loco disease of rabbits. Eighteen rabbits were randomly separated into control group, poisoning group and curing group, with six rabbits in each group. Rabbits in poisoning group and curing group were fed 10 g/（kg ~ d） powder of Oxytropis glabra DC every morning for 70 d till the rabbits in poisoning group began to die. From the 21＇t to 24＇h d of O. glabra administration, each of the rabbits in curing group was injected with 1 mL of polyclonal anti- body against SW every day. Blood samples were collected before O. glabra challenge, and then once every 7 d for the serological and immunological evaluation of serum. The resuhs showed that the serum alkaline phosphatase （AKP） activity and SW content in poisoning groups were significantly higher than those in control group on the 7th d （P 〈0.05 ） ; the serum aspartate transaminase （AST） and lactate dehydrogenase （LDH） activity in poisoning groups were significantly higher than those in control group on the 14th d （P 〈 0.05 ）, while the serum ct - mannosidase （AMA） and rate of E-rosette formation in poisoning groups were signifi- cantly lower than those in control group （ P 〈 0.05 ） ; the serum blood urea nitrogen （BUN）, creatinine （ CRE ） and glucose （ GLU ） concentration in poisoning groups were significantly higher than those in control group on the 21＇t d （P 〈 0.05 ）. By injecting serum against swainsonine, the serum AKP, LDH, AST, alanine transaminase （ALT） activity and contents of BUN, CRE, GLU, SW were reduced and serum AMA and rate of E-rosette formation were increased in curing group, by comparing with those in poisoning group. These results in this experiment indicated that polyclonal antibody against SW had good value of preventing from O. glabra DC.

家兔苦马豆素多克隆抗体的制备及其对小花棘豆中毒的治疗效果

采用合成的苦马豆素(swainsonine,SW)人工抗原免疫接种家兔,获得高效价的SW抗血清,分离纯化得到的家兔SW多克隆抗体,复制家兔小花棘豆中毒模型,用SW多克隆抗体治疗小花棘豆中毒家兔,通过检测血清生化指标和免疫学指标,评价SW多克隆抗体治疗家兔小花棘豆中毒的效果。将18只家兔随机分为对照组(6只)、攻毒组(6只)和攻毒治疗组(6只),攻毒组和攻毒治疗组按照10g/(kg·d)的剂量饲喂小花棘豆,攻毒组试验兔出现死亡时(第70天)停止攻毒。攻毒第21天,攻毒治疗组试验兔注射SW多克隆抗体,每只兔每天1mL,连续注射4d。以攻毒试验开始为第0天进行首次采血,试验开始后每7d采血1次,进行血清免疫和生化指标测定。结果显示,攻毒第7天时攻毒家兔血清AKP活性和SW质量浓度显著高于对照家兔(P<0.05);第14天时攻毒家兔血清AST和LDH活性显著高于对照家兔(P<0.05),血清AMA活性和E-玫瑰花环率均显著低于对照家兔(P<0.05);第21天时攻毒家兔血清BUN、CRE和GLU浓度显著高于对照组家兔(P<0.05)。攻毒治疗组家兔注射SW抗血清后血清AKP、LDH、AST、ALT活性,BUN、CRE、GLU浓度和SW质量浓度较攻毒家兔显著下降,AMA活性和E-玫瑰花环率显著上升。说明,SW多克隆抗体可有效治疗家兔小花棘豆中毒。