Current seasonal influenza vaccines confer only limited coverage of virus strains due to the frequent genetic and antigenic variability of influenza virus(IV).Epitope vaccines that accurately target conserved domains ...Current seasonal influenza vaccines confer only limited coverage of virus strains due to the frequent genetic and antigenic variability of influenza virus(IV).Epitope vaccines that accurately target conserved domains provide a promising approach to increase the breadth of protection;however,poor immunogenicity greatly hinders their application.The protruding(P)domain of the norovirus(NoV),which can self-assemble into a 24-mer particle called the NoV P particle,offers an ideal antigen presentation platform.In this study,a multiepitope nanovaccine displaying influenza epitopes(HMN-PP)was constructed based on the NoV P particle nanoplatform.Large amounts of HMN-PP were easily expressed in Escherichia coli in soluble form.Animal experiments showed that the adjuvanted HMN-PP nanovaccine induced epitope-specific antibodies and haemagglutinin(HA)-specific neutralizing antibodies,the antibodies could persist for at least three months after the last immunization.Furthermore,HMN-PP induced matrix protein 2 extracellular domain(M2e)-specific antibody-dependent cell-mediated cytotoxicity,CD4^(+)and CD8^(+)T-cell responses,a nucleoprotein(NP)-specific cytotoxic T lymphocyte(CTL)response.These results indicated that the combination of a multiepitope vaccine and self-assembled NoV P particles may be an ideal and effective vaccine strategy for highly variable viruses such as IV and SARS-CoV-2.展开更多
The hardness values and the wear resistance of Al2O3P/ Zn-Al composite, prepared by means of rheological casting technology,are investigated separately in this work. The results show that the addition of Al2O3P increa...The hardness values and the wear resistance of Al2O3P/ Zn-Al composite, prepared by means of rheological casting technology,are investigated separately in this work. The results show that the addition of Al2O3P increases the hardness values of the matrix at both room and high temperature and improves the wear resistance of the material.The hardness values and the wear resistance of the composite rise with the increase of the particle volume fraction or the decrease of the particle size.The raising of test temperature results in a rapid descending of its hardness values.However, the addition of Al2O3P improves the property of high temperature resistance of Zn-Al alloys significantly.Moreover,the effect of quenching, tempering or cycling heat treatment on the hardness values of the composite is also studied.展开更多
Using SEM and EDS some particles containing La and P were discovered in low oxygen and sulfur purity steel with lanthanum. In these particles low melting point elements such as As and Sb also were detected. According ...Using SEM and EDS some particles containing La and P were discovered in low oxygen and sulfur purity steel with lanthanum. In these particles low melting point elements such as As and Sb also were detected. According to the result of simulative calculation of segregation, it is shown that La and P enrich so strongly in the process of solidification that their concentrations can satisfy thermodynamic condition to forme LaP compound or meet the requirements to forme eutectic phase in the last stage of solidification. Therefore, the particles are concluded as LaP compound or eutectics, which exist on grain boundary mainly.展开更多
基金the Department of Science and Technology of Jilin Province(Nos.20220204008YY and 20210204197YY)Changchun Science and Technology Bureau(No.21ZY15).
文摘Current seasonal influenza vaccines confer only limited coverage of virus strains due to the frequent genetic and antigenic variability of influenza virus(IV).Epitope vaccines that accurately target conserved domains provide a promising approach to increase the breadth of protection;however,poor immunogenicity greatly hinders their application.The protruding(P)domain of the norovirus(NoV),which can self-assemble into a 24-mer particle called the NoV P particle,offers an ideal antigen presentation platform.In this study,a multiepitope nanovaccine displaying influenza epitopes(HMN-PP)was constructed based on the NoV P particle nanoplatform.Large amounts of HMN-PP were easily expressed in Escherichia coli in soluble form.Animal experiments showed that the adjuvanted HMN-PP nanovaccine induced epitope-specific antibodies and haemagglutinin(HA)-specific neutralizing antibodies,the antibodies could persist for at least three months after the last immunization.Furthermore,HMN-PP induced matrix protein 2 extracellular domain(M2e)-specific antibody-dependent cell-mediated cytotoxicity,CD4^(+)and CD8^(+)T-cell responses,a nucleoprotein(NP)-specific cytotoxic T lymphocyte(CTL)response.These results indicated that the combination of a multiepitope vaccine and self-assembled NoV P particles may be an ideal and effective vaccine strategy for highly variable viruses such as IV and SARS-CoV-2.
文摘The hardness values and the wear resistance of Al2O3P/ Zn-Al composite, prepared by means of rheological casting technology,are investigated separately in this work. The results show that the addition of Al2O3P increases the hardness values of the matrix at both room and high temperature and improves the wear resistance of the material.The hardness values and the wear resistance of the composite rise with the increase of the particle volume fraction or the decrease of the particle size.The raising of test temperature results in a rapid descending of its hardness values.However, the addition of Al2O3P improves the property of high temperature resistance of Zn-Al alloys significantly.Moreover,the effect of quenching, tempering or cycling heat treatment on the hardness values of the composite is also studied.
基金Project supported by the Ministry of Science and Technology of China (2002BA315A-5)
文摘Using SEM and EDS some particles containing La and P were discovered in low oxygen and sulfur purity steel with lanthanum. In these particles low melting point elements such as As and Sb also were detected. According to the result of simulative calculation of segregation, it is shown that La and P enrich so strongly in the process of solidification that their concentrations can satisfy thermodynamic condition to forme LaP compound or meet the requirements to forme eutectic phase in the last stage of solidification. Therefore, the particles are concluded as LaP compound or eutectics, which exist on grain boundary mainly.
