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Effects of metal lead on growth and mycorrhizae of an invasive plant species (Solidago canadensis L.) 被引量:14
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作者 YANG Ruyi YU Guodong +1 位作者 TANG Jianjun CHEN Xin 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2008年第6期739-744,共6页
It is less known whether and how soil metal lead (Pb) impacts the invasion of exotic plants. A greenhouse experiment was conducted to estimate the effects of lead on the growth and mycorrhizae of an invasive species... It is less known whether and how soil metal lead (Pb) impacts the invasion of exotic plants. A greenhouse experiment was conducted to estimate the effects of lead on the growth and mycorrhizae of an invasive species (Solidago canadensis L.) in a microcosm system. Each microcosm unit was separated into HOST and TEST compartments by a replaceable mesh screen that allowed arbuscular mycorrhizal (AM) fungal hyphae rather than plant roots to grow into the TEST compartments. Three Pb levels (control, 300, and 600 mg/kg soil) were used in this study to simulate ambient soil and two pollution sites where S. canadensis grows. Mycorrhizal inoculum comprised five indigenous arbuscular mycorrhizal fungal species ( Glomus mosseae, Glomus versiform, Glomus diaphanum, Glomus geosporum, and Glomus etunicatum). The ^15N isotope tracer was used to quantify the mycorrhizally mediated nitrogen acquisition of plants. The results showed that S. canadensis was highly dependent on mycorrhizae. The Pb additions significantly decreased biomass and arbuscular mycorrhizal colonization (root length colonized, RLC%) but did not affect spore numbers, N (including total N and ^15N) and P uptake. The facilitating efficiency of mycorrhizae on nutrient acquisition was promoted by Pb treatments. The Pb was mostly sequestered in belowground of plant (root and rhizome). The results suggest that the high efficiency of mycorrhizae on nutrient uptake might give S. canadensis a great advantage over native species in Pb polluted soils. 展开更多
关键词 Solidago canadensis L. metal lead MYCORRHIZAE n and p uptake pb accumulation
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荷兰3016杨耐盐变异体的筛选 被引量:2
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作者 纪纯阳 彭儒胜 +8 位作者 梁德军 胡伟平 王伟 赵洪波 矫丽曼 赵继梅 赵鑫闻 李晓宇 王敏 《西部林业科学》 CAS 北大核心 2019年第6期29-34,40,共7页
以荷兰3016杨一年生健壮无病虫害的扦插苗为试验材料,对其叶柄诱导形成的愈伤组织,采用固体培养和液体培养2种方式进行耐NaCl变异体筛选,建立其高效的无菌苗再生体系。结果表明,固体培养方式通过有盐培养→无盐培养→有盐培养的选择程序... 以荷兰3016杨一年生健壮无病虫害的扦插苗为试验材料,对其叶柄诱导形成的愈伤组织,采用固体培养和液体培养2种方式进行耐NaCl变异体筛选,建立其高效的无菌苗再生体系。结果表明,固体培养方式通过有盐培养→无盐培养→有盐培养的选择程序,获得了耐0.2%和0.4%NaCl水平的不定芽,对不定芽进行生根诱导,获得了完整植株。液体培养方式获得了耐0.2%和0.4%NaCl水平的悬浮细胞系,悬浮细胞系经植板培养未能形成愈伤组织。 展开更多
关键词 荷兰3016 耐盐变异体 固体培养 液体培养
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