清肾颗粒对大鼠NRK-52E细胞转分化模型miR-23b和PINK1/Parkin通路的影响

目的 探讨TGF-β1诱导大鼠NRK-52E细胞转分化模型中miR-23b对PINK1/Parkin通路的靶向调节机制,并阐明清肾颗粒含药血清对NRK-52E细胞转分化的干预机理。方法 采用超高效液相色谱(UPLC)指纹图谱法对清肾颗粒进行全指纹图谱分析。构建TGF-β1诱导大鼠NRK-52E细胞转分化模型,转染siRNA后分为模拟物空载对照组、miR-23b-5p模拟物组、抑制剂空载对照组、miR-23b-5p抑制剂组,观察miR-23b-5p对PINK1表达量的影响。再将NRK-52E细胞分组为正常组、TGF-β1组、清肾颗粒组、miR-23b-mimic-NC组、miR-23b-mimic组、miR-23b-mimic+清肾颗粒组,Western blot法检测NRK-52E细胞中Pink1、Parkin、LC3Ⅱ、Beclin-1、P62、α-SMA蛋白表达,RT-qPCR法检测NRK-52E细胞中miR-23b-5p、Pink1、Parkin、Beclin-1、α-SMA mRNA的表达,双荧光素酶报告基因实验检测miR-23b-5p与PINK1的靶向关系。结果 UPLC指纹图谱法鉴定出清肾颗粒中11个活性成分。miR-23b-5p过表达后,PINK1 mRNA表达量也显著增加(P<0.05);而miR-23b-5p表达沉默后,PINK1 mRNA表达量也显著减少(P<0.05)。双荧光素酶报告显示,Rno-miR-23b-5p能显著下调Rno-PINK1-WT荧光素酶活性(P<0.05),但未能下调突变Rno-PINK1-mut荧光素酶活性(P>0.05)。清肾颗粒含药血清干预实验发现,TGF-β1组的miR-23b-5p、Pink1、Parkin、Beclin-1、LC3Ⅱ表达及LC3Ⅱ/Ⅰ比值均明显低于正常组,P62和α-SMA表达明显高于正常组(P<0.05)。清肾颗粒组和miR-23b-mimic组的miR-23b-5p、Pink1、Parkin、Beclin-1、LC3Ⅱ表达及LC3Ⅱ/Ⅰ比值均明显高于TGF-β1组,P62和α-SMA表达明显低于TGF-β1组(P<0.05)。miR-23b-mimic+清肾颗粒组的表现更优于miR-23b-mimic组(P<0.05)。结论 清肾颗粒能够上调NRK-52E细胞内miR-23b-5p表达,并通过增强PINK1/Parkin通路介导的线粒体自噬活性,抑制NRK-52E细胞转分化进程。

Introduction of Internet P.E News Reporter

Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant with personal information.

P.E Science J ournals General Influence Factor

The portal is characterized with large amount of information, high stability and interaction, while "Online Journal of Physical P.E" is to publicize the academic pure value, spread the achievement influence in daily life, and advocate an academic atmosphere of utilitarianism with a slogan of "article issued online can be published in journal with the recommendation of experts" and practical action. It is the first academic journal under network operation.

Linc01419调控miR-34a-5p/E2F3轴促进膀胱癌细胞增殖与侵袭

目的:探讨长链非编码RNA Linc01419对膀胱癌细胞增殖和侵袭的影响及作用机制。方法:通过UALCAN软件(https://ualcan.path.uab.edu/)分析TCGA数据库中Linc01419在膀胱癌组织与正常膀胱组织中的表达差异;采用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RT-qPCR)检测Linc01419在不同膀胱癌细胞系、22例经病理证实为膀胱癌的手术患者的肿瘤组织及癌旁正常膀胱组织中的表达水平;应用细胞增殖/毒性检测(cell counting kit-8,CCK-8)实验和Transwell小室侵袭实验检测敲低Linc01419表达对膀胱癌细胞增殖与侵袭的影响;采用双荧光素酶报告基因检测法分析Linc01419与miR-34a-5p及miR-34a-5p与E2F3之间的靶向调控关系。结果:UALCAN数据库分析显示相较正常膀胱组织,Linc01419在膀胱癌组织中显著高表达(P<0.001);RT-qPCR分析结果显示相较癌旁正常膀胱组织,Linc01419在22例膀胱癌组织中表达明显上调(P<0.001),与UALCAN数据库分析结果一致;Linc01419在4株膀胱癌细胞中的表达明显高于正常膀胱上皮细胞(P<0.01);敲低Linc01419表达,可显著抑制膀胱癌细胞的增殖、侵袭及N-cadherin、PCNA蛋白的表达,而显著促进E-cadherin的蛋白表达(P<0.05);miR-34a-5p过表达对膀胱癌细胞具有类似的抑制作用;双荧光素酶报告基因实验、RIP及Pull-down实验证实Linc01419可靶向结合miR-34a-5p,而后者进一步介导了对E2F3的靶向调控;抑制miR-34a-5p表达,可显著削弱Linc01419沉默对膀胱癌细胞生物学行为及E-cadherin、N-cadherin、PCNA、E2F3表达的影响。结论:Linc01419在膀胱癌中异常高表达,其通过调控miR-34a-5p/E2F3轴促进膀胱癌细胞增殖和侵袭,很可能是膀胱癌发生、发展过程中的一个重要环节。

Student Evaluations as Effective Indicators of Learning Outcomes:Evidence from Japanese University Students in English Language Courses

The term"student learning outcomes"refers to the knowledge, skills, and abilitiesthat students achieve during a course, and is typically assessed based on student evaluations conducted at the end of the semester. Previous studies in this area have investigated the effects of instructional quality and academic demands separately and have been limited primarily to examining findings using student samples fromthe United States. With Japanese college students' perceptions of self-improvementin English language courses as the dependent variable, the present study directly tests the hypothesis that students who perceive instructional quality to be higher, andcourse demands to be greater, also estimate higher levels of self-improvement in English language skills. The analysis provides strong support for this hypothesis.Higher ratings of instruction and academic demands have already beenshown to increase levels of student learning(Greimel-Fuhrmann and Geyer 2003;Nois and Hudson 2006; Mc Fadden and Dart 1992). The present study is the first toprovide direct evidence of the relative importance of student evaluations ofinstructional quality and academic demands as predictors of student learning and thefirst ever to do so with a sample of Japanese college students enrolled in a required English as a foreign language course. Our hypothesis is that Japanese students whoperceive instructional quality to be higher, and course demands to be greater,estimate higher levels of self-improvement in English language skills. Thus we test Japanese students' attitudes toward instructional quality and course demands asindependent variables predicting their perceptions of self-improvement in English language courses. The research focuses on Japanese students' improvement in English language skills because English education in Japan is an arena in which thedebate over limited English proficiency rages on, and because other research suggests reconsideration of English education in light of the demands of the rapidly expanding global era(Amaki 2008).

Analysis on the Optimization of the P.E. Course Teaching Plan in Colleges and Universities

In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students＇ learning efficiency. In this paper, the current situation of the current physical education is analyzed, and also related suggestions on the optimization of the P.E. course teaching plan are proposed.

MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1-extracellular signal-regulated kinase-1/2 axis

BACKGROUND MicroRNAs(miRNAs)regulate gene expression and play a critical role in cancer physiology.However,there is still a limited understanding of the function and regulatory mechanism of miRNAs in gastric cancer(GC).AIM To investigate the role and molecular mechanism of miRNA-145-5p(miR145-5p)in the progression of GC.METHODS Real-time polymerase chain reaction(RT-PCR)was used to detect miRNA expression in human GC tissues and cells.The ability of cancer cells to migrate and invade was assessed using wound-healing and transwell assays,respectively.Cell proliferation was measured using cell counting kit-8 and colony formation assays,and apoptosis was evaluated using flow cytometry.Expression of the epithelial-mesenchymal transition(EMT)-associated protein was determined by Western blot.Targets of miR-145-5p were predicated using bioinformatics analysis and verified using a dual-luciferase reporter system.Serpin family E member 1(SERPINE1)expression in GC tissues and cells was evaluated using RT-PCR and immunohistochemical staining.The correlation between SERPINE1 expression and overall patient survival was determined using Kaplan-Meier plot analysis.The association between SERPINE1 and GC progression was also tested.A rescue experiment of SERPINE1 overexpression was conducted to verify the relationship between this protein and miR-145-5p.The mechanism by which miR-145-5p influences GC progression was further explored by assessing tumor formation in nude mice.RESULTS GC tissues and cells had reduced miR-145-5p expression and SERPINE1 was identified as a direct target of this miRNA.Overexpression of miR-145-5p was associated with decreased GC cell proliferation,invasion,migration,and EMT,and these effects were reversed by forcing SERPINE1 expression.Kaplan-Meier plot analysis revealed that patients with higher SERPINE1 expression had a shorter survival rate than those with lower SERPINE1 expression.Nude mouse tumorigenesis experiments confirmed that miR-145-5p targets SERPINE1 to regulate extracellular signal-regulated kinase-1/2(ERK1/2).CONCLUSION This study found that miR-145-5p inhibits tumor progression and is expressed in lower amounts in patients with GC.MiR-145-5p was found to affect GC cell proliferation,migration,and invasion by negatively regulating SERPINE1 levels and controlling the ERK1/2 pathway.

Exercise-induced modulation of miR-149-5p and MMP9 in LPS-triggered diabetic myoblast ER stress: licorice glycoside E as a potential therapeutic target

Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeutic drugs and targets for diabetes-related sepsis.The research also incorporates traditional physical therapy perspectives,emphasizing the genomic insights gained from exercise therapy in disease management and prevention.Methods:Gene analysis was conducted on the GSE168796 and GSE94717 datasets to identify ER stress-related genes.Gene interactions and immune cell correlations were mapped using GeneCard and STRING databases.A screening of 2,456 compounds from the TCMSP database was performed to identify potential therapeutic agents,with a focus on their docking potential.Techniques such as luciferase reporter gene assay and RNA interference were used to examine the interactions between microRNA-149-5p and MMP9.Results:The study identified 2,006 differentially expressed genes and 616 miRNAs.Key genes like MMP9,TNF-α,and IL1B were linked to an immunosuppressive state.Licorice glycoside E demonstrated high affinity for MMP9,suggesting its potential effectiveness in treating diabetes.The constructed miRNA network highlighted the regulatory roles of MMP9,IL1B,IFNG,and TNF-α.Experimental evidence confirmed the binding of microRNA-149-5p to MMP9,impacting apoptosis in diabetic cells.Conclusion:The findings highlight the regulatory role of microRNA-149-5p in managing MMP9,a crucial gene in diabetes pathophysiology.Licorice glycoside E emerges as a promising treatment option for diabetes,especially targeting MMP9 affected by ER stress.The study also underscores the significance of physical exercise in modulating ER stress pathways in diabetes management,bridging traditional physical therapy and modern scientific understanding.Our study has limitations.It focuses on the microRNA-149-5p-MMP9 network in sepsis,using cell-based methods without animal or clinical trials.Despite strong in vitro findings,in vivo studies are needed to confirm licorice glycoside E’s therapeutic potential and understand the microRNA-149-5p-MMP9 dynamics in real conditions.

Attitude, Perception and Use of E-Learning at Open University Malaysia

To ensure the success of the e-learning initiatives,OUM has developed its own e-learning management system,known as myLMS.Since its introduction,many modifications and improvements have been introduced to increase its effectiveness.It is now timely that OUM take stock of its students'attitudes towards e-learning.Thus,a survey was conducted on about 1,000 students at one of OUM's own learning centres,that is,the Kelantan Regional Centrel.The study indicated that generally the teacher cohort had a somewhat neutral attitude towards e-learning.The use of e-learning was more specifically aimed at achieving short term goals of obtaining good coursework and examination grades by capitalizing on the use of the Discussion Board and Courseware.A closer examination reveals that the females prefer the Discussion Board while the males prefer the Courseware.Learners in the Engineering and English programmes had more positive attitudes towards e-learning compared to learners in the Mathematics and Science programme. Learners with CGPA＞3.0 who are categorized as high achievers are more positive towards e-learning as compared to the low achievers(CGPA＜3.0).Age difference,learners‘income per month,learners’Internet and e-learning habits were also found to be predictors of attitude towards e-learning.

Research and Practice of the P.E. Information Teaching Design System

In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database （nine modules in total） on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency.

Interview With Prof. Ved P. Nanda, School of Law, University of Denver, USA

Editor's Note: On Sept 9, the staff reporter of Human Rights magazine You Xueyun interviewed Prof. Ved P. Nanda, School of Law, University of Denver, USA and Honorary President of World Jurist Association (WJA). He attended the 22nd Congress on the Law of the World held by WJA in Beijing and Shanghai, China, from Sept.4-10. Following is his viewpoints on human rights.

A Study on the Impact of Learning Management System on Students of the University of Jordan

Despite of the advantages of Information and Communication Technology (ICT) which makes our lives easier, faster, and more connected, the development of ICT is pushing the countries in the direction of ICT applications. The higher education is one of the sectors that try to adopt one of ICT applications through E-learning and using (Moodle) as learning management system. This paper finds out the impact of Moodle on students through examining the students’ acceptance of the system using TAM model. This study was carried out by some teaching members of the Faculty of King Abdullah II School for Information Technology at the University of Jordan during the spring semester of the academic year 2013/2014. The results of this study firm the original TAM’s findings and reveal that the faculties of students and number of previously E-learning courses have an influence on perceive ease of use and perceived usefulness while the level of the academic year and GPA have no significant influence on perceived ease of use. Even though, they have affected on perceived usefulness. Finally, the student’s skills on computer with student’s difficulty in reading from the screen affect perceived ease of use but, it has been found that they have no influence on perceived usefulness.

The Impact of Electronic Cigarettes (e-Cigs) Smoking Habit on Periodontal Status and Salivary pH among Some Passive e-Cigs Smokers Referred to the College of Dentistry Clinics, King Khalid University

Background: Electronic cigarette (e-cigs) smoking is substitutional to traditional cigarette smoking to reduce the dangerous combustion of products. Moreover, passive smoking is involuntarily tobacco smoking due to the exposure to cigarette or tobacco smoke among non-smokers and due to there being little knowledge about the impact of passive e-cigs smoking on periodontal status and salivary pH. Therefore, the present study aimed to evaluate the effect of e-cigs smoking habit on periodontal tissue and salivary pH among some passive e-cigs smokers referred to the college of dentistry clinics, King Khalid University. Material and Methods: Ninety male participants who were referred to the college of dentistry clinics at King Khalid University were included in the study. Age, gender, e-cigs smoking, and general health were recorded. The participants were divided into three equal groups (n = 30) as follows: Group I (Non-passive e-cigs smokers and non-smokers) as the control group, Group II (e-cigs users), and Group III (Passive e-cigs smokers). Salivary pH, plaque control record (PCR), gingival bleeding index (GBI), clinical attachment loss (CAL), percentage of radiographic bone loss (% RBL), periodontal pocket depth (PPD), more than 5 missing teeth due to periodontal diseases (>5 MTDP), tooth mobility (TM), furcation involvement (FI), Bite collapse (BC), and less than 20 remaining teeth (10 Opposing pairs) (L20RT) as well as HbA1c were recorded. ANOVA test was used to the comparison between Groups I, II, and III in the participants’ ages and periodontitis staging clinical findings. The mean of participants’ age groups, the mean of salivary pH values of study groups, and the periodontitis staging complexity and HbA1c were compared between groups with the ANOVA test, Tukey’s test, and the chi-square test. P-value was recorded, and less than 0.5 was considered a statistically significant difference (p Results: The e-cigs users group revealed higher means of PCR, GBI %RBL values, and the participants percentages of >5 MTDP, TM, FI, L20RT, and diabetes mellitus (DM) among participants compared to the passive e-cigs smokers group and control group except for the participants percentage of BC among the participants, which was higher among the control group participants. The differences were not significant in PCR, GBI, %RBL and DM (p > 0.5) and significant in >5 MTDP, TM, FI, L20RT and smoking (p 7% values compared to the e-cigs users group and control group participants. The differences were not significant in CAL and PPD (p > 0.5) and significant in the participants percentages of salivary pH values (p Conclusion: The e-cigs smoking habit was the cause of an increase in periodontal disease severity among the electronic smokers rather than passive e-cigs smokers, although the salivary pH was higher in the latter.

Faculty Members’ Attitudes and Perceptions towards E-Publishing at the University of Jordan

Electronic Publishing offers many opportunities to today’s users and scholars that were not available to their predecessors. Because of the multidimensional features of e-Journals, they are becoming the choice of academic as well as public library users. Technology, and particularly the Internet, has great potential for altering scholarly communication and research inhumanities, sciences, social sciences, and health sciences disciplines. Results of scholarly surveys show that there is a significant interest in e-Publication of research. Thus, this research paper investigates the attitudes and perceptions of faculty members at the University of Jordan (UJ) of current issues associated with the use of electronic publishing and perceived obstacles to their extended use for scholarly communication and publication. Specifically, the primary focus of this study is to examine and perceive how faculty members’ use of electronic publications might affect their interest in publishing their own research in electronic formats;as well as to determine the contribution of scholars towards publishing in e-Journals. In order to achieve the research objectives, a questionnaire method was adopted to elicit the opinions of the faculty members;whereas personal interviews were conducted to fill in the gaps. The results of the study reveal that the majority of the faculty members prefer to publish in electronic formats refereed journals, due to the advantages of publishing in e-Journals. Furthermore, the findings show that faculty members are also concerned about the disadvantages of e-Publishing. On the basis of the findings, some suggestions have been recommended to make publishing in e-Journals more acceptable to those scholars at UJ who still do not use it, as well as to maximize the use of e-Publishing because of the advantages that it may offer.

Functionalized selenium nanoparticles ameliorated acetaminophen-induced hepatotoxicity through synergistically triggering PKCδ/Nrf2 signaling pathway and inhibiting CYP 2E1

Selenium nanoparticles(SeNPs)have been demonstrated potential for use in diseases associated with oxidative stress.Functionalized SeNPs with lower toxicity and higher biocompatibility could bring better therapeutic activity and clinical application value.Herein,this work was conducted to investigate the protective effect of Pleurotus tuber-regium polysaccharide-protein complex funtionnalized SeNPs(PTR-SeNPs)against acetaminophen(APAP)-induced oxidative injure in HepG2 cells and C57BL/6J mouse liver.Further elucidation of the underlying molecular mechanism,in particular their modulation of Nrf2 signaling pathway was also performed.The results showed that PTR-SeNPs could significantly ameliorate APAP-induced oxidative injury as evidenced by a range of biochemical analysis,histopathological examination and immunoblotting study.PTR-SeNPs could hosphorylate and activate PKCδ,depress Keap1,and increase nuclear accumulation of Nrf2,resulting in upregulation of GCLC,GCLM,HO-1 and NQO-1 expression.Besides,PTR-SeNPs suppressed the biotransformation of APAP to generate intracellular ROS through CYP 2E1 inhibition,restoring the mitochondrial morphology.Furthermore,the protective effect of PTR-SeNPs against APAP induced hepatotoxicity was weakened as Nrf2 was depleted in vivo,indicating the pivotal role of Nrf2 signaling pathway in PTR-SeNPs mediated hepatoprotective efficacy.Being a potential hepatic protectant,PTR-SeNPs could serve as a new source of selenium supplement for health-promoting and biomedical applications.

Rational and Continuous Measurement of the Emotional Decision Making in Visual Recognition of Facial Emotional Expressions with M.A.R.I.E.: First Half

Context: The advent of Artificial Intelligence (AI) requires modeling prior to its implementation in algorithms for most human skills. This observation requires us to have a detailed and precise understanding of the interfaces of verbal and emotional communications. The progress of AI is significant on the verbal level but modest in terms of the recognition of facial emotions even if this functionality is one of the oldest in humans and is omnipresent in our daily lives. Dysfunction in the ability for facial emotional expressions is present in many brain pathologies encountered by psychiatrists, neurologists, psychotherapists, mental health professionals including social workers. It cannot be objectively verified and measured due to a lack of reliable tools that are valid and consistently sensitive. Indeed, the articles in the scientific literature dealing with Visual-Facial-Emotions-Recognition (ViFaEmRe), suffer from the absence of 1) consensual and rational tools for continuous quantified measurement, 2) operational concepts. We have invented a software that can use computer-morphing attempting to respond to these two obstacles. It is identified as the Method of Analysis and Research of the Integration of Emotions (M.A.R.I.E.). Our primary goal is to use M.A.R.I.E. to understand the physiology of ViFaEmRe in normal healthy subjects by standardizing the measurements. Then, it will allow us to focus on subjects manifesting abnormalities in this ability. Our second goal is to make our contribution to the progress of AI hoping to add the dimension of recognition of facial emotional expressions. Objective: To study: 1) categorical vs dimensional aspects of recognition of ViFaEmRe, 2) universality vs idiosyncrasy, 3) immediate vs ambivalent Emotional-Decision-Making, 4) the Emotional-Fingerprint of a face and 5) creation of population references data. Methods: With M.A.R.I.E. enable a rational quantified measurement of Emotional-Visual-Acuity (EVA) of 1) a) an individual observer, b) in a population aged 20 to 70 years old, 2) measure the range and intensity of expressed emotions by 3 Face-Tests, 3) quantify the performance of a sample of 204 observers with hyper normal measures of cognition, “thymia,” (ibid. defined elsewhere) and low levels of anxiety 4) analysis of the 6 primary emotions. Results: We have individualized the following continuous parameters: 1) “Emotional-Visual-Acuity”, 2) “Visual-Emotional-Feeling”, 3) “Emotional-Quotient”, 4) “Emotional-Deci-sion-Making”, 5) “Emotional-Decision-Making Graph” or “Individual-Gun-Trigger”6) “Emotional-Fingerprint” or “Key-graph”, 7) “Emotional-Finger-print-Graph”, 8) detecting “misunderstanding” and 9) detecting “error”. This allowed us a taxonomy with coding of the face-emotion pair. Each face has specific measurements and graphics. The EVA improves from ages of 20 to 55 years, then decreases. It does not depend on the sex of the observer, nor the face studied. In addition, 1% of people endowed with normal intelligence do not recognize emotions. The categorical dimension is a variable for everyone. The range and intensity of ViFaEmRe is idiosyncratic and not universally uniform. The recognition of emotions is purely categorical for a single individual. It is dimensional for a population sample. Conclusions: Firstly, M.A.R.I.E. has made possible to bring out new concepts and new continuous measurements variables. The comparison between healthy and abnormal individuals makes it possible to take into consideration the significance of this line of study. From now on, these new functional parameters will allow us to identify and name “emotional” disorders or illnesses which can give additional dimension to behavioral disorders in all pathologies that affect the brain. Secondly, the ViFaEmRe is idiosyncratic, categorical, and a function of the identity of the observer and of the observed face. These findings stack up against Artificial Intelligence, which cannot have a globalist or regionalist algorithm that can be programmed into a robot, nor can AI compete with human abilities and judgment in this domain. *Here “Emotional disorders” refers to disorders of emotional expressions and recognition.

Rational and Continuous Measurement of Emotional-Fingerprint, Emotional-Quotient and Categorical vs Proportional Recognition of Facial Emotions with M.A.R.I.E., Second Half

Context: The advent of Artificial Intelligence (AI) requires modeling prior to its implementation in algorithms for most human skills. This observation requires us to have a detailed and precise understanding of the interfaces of verbal and emotional communications. The progress of AI is significant on the verbal level but modest in terms of the recognition of facial emotions even if this functionality is one of the oldest in humans and is omnipresent in our daily lives. Dysfunction in the ability for facial emotional expressions is present in many brain pathologies encountered by psychiatrists, neurologists, psychotherapists, mental health professionals including social workers. It cannot be objectively verified and measured due to a lack of reliable tools that are valid and consistently sensitive. Indeed, the articles in the scientific literature dealing with Visual-Facial-Emotions-Recognition (ViFaEmRe), suffer from the absence of 1) consensual and rational tools for continuous quantified measurement, 2) operational concepts. We have invented a software that can use computer-morphing attempting to respond to these two obstacles. It is identified as the Method of Analysis and Research of the Integration of Emotions (M.A.R.I.E.). Our primary goal is to use M.A.R.I.E. to understand the physiology of ViFaEmRe in normal healthy subjects by standardizing the measurements. Then, it will allow us to focus on subjects manifesting abnormalities in this ability. Our second goal is to make our contribution to the progress of AI hoping to add the dimension of recognition of facial emotional expressions. Objective: To study: 1) categorical vs dimensional aspects of recognition of ViFaEmRe, 2) universality vs idiosyncrasy, 3) immediate vs ambivalent Emotional-Decision-Making, 4) the Emotional-Fingerprint of a face and 5) creation of population references data. Methods: M.A.R.I.E. enables the rational, quantified measurement of Emotional Visual Acuity (EVA) in an individual observer and a population aged 20 to 70 years. Meanwhile, it can measure the range and intensity of expressed emotions through three Face- Tests, quantify the performance of a sample of 204 observers with hypernormal measures of cognition, “thymia” (defined elsewhere), and low levels of anxiety, and perform analysis of the six primary emotions. Results: We have individualized the following continuous parameters: 1) “Emotional-Visual- Acuity”, 2) “Visual-Emotional-Feeling”, 3) “Emotional-Quotient”, 4) “Emotional-Decision-Making”, 5) “Emotional-Decision-Making Graph” or “Individual-Gun-Trigger”, 6) “Emotional-Fingerprint” or “Key-graph”, 7) “Emotional-Fingerprint-Graph”, 8) detecting “misunderstanding” and 9) detecting “error”. This allowed us a taxonomy with coding of the face-emotion pair. Each face has specific measurements and graphics. The EVA improves from ages of 20 to 55 years, then decreases. It does not depend on the sex of the observer, nor the face studied. In addition, 1% of people endowed with normal intelligence do not recognize emotions. The categorical dimension is a variable for everyone. The range and intensity of ViFaEmRe is idiosyncratic and not universally uniform. The recognition of emotions is purely categorical for a single individual. It is dimensional for a population sample. Conclusions: Firstly, M.A.R.I.E. has made possible to bring out new concepts and new continuous measurements variables. The comparison between healthy and abnormal individuals makes it possible to take into consideration the significance of this line of study. From now on, these new functional parameters will allow us to identify and name “emotional” disorders or illnesses which can give additional dimension to behavioral disorders in all pathologies that affect the brain. Secondly, the ViFaEmRe is idiosyncratic, categorical, and a function of the identity of the observer and of the observed face. These findings stack up against Artificial Intelligence, which cannot have a globalist or regionalist algorithm that can be programmed into a robot, nor can AI compete with human abilities and judgment in this domain. *Here “Emotional disorders” refers to disorders of emotional expressions and recognition.

HPV E6/E7mRNA检测与P16/Ki-67免疫组化检测对宫颈CIN2级病变的筛查价值探究

目的:研究人乳头瘤病毒(Human papilloma virus,HPV)E6/E7mRNA与免疫组化抑癌基因P16/细胞增殖核抗原Ki-67在宫颈上皮内瘤样病变(Cervical intraepithelial neoplasia,CIN)2级病变筛查中的应用价值。方法:选取2020年2月至2023年2月期间本院收治的92例宫颈炎症及病变患者作为研究对象。根据病理检查结果,将CIN2级患者纳入研究组(n=45),宫颈CIN1级和宫颈炎患者纳入对照组(n=47)。对比两组HPV E6/E7mRNA、P16/Ki-67单独及联合检测阳性表达率差异,采用Logistic回归分析影响CIN2诊断的危险因素,并采用受试者工作曲线分析HPV E6/E7mRNA与P16/Ki-67单独及联合检测在宫颈CIN2级病变筛查的价值。结果:研究组HPV E6/E7mRNA、P16/Ki-67单独检测阳性率、联合检测阳性率(82.22%,77.78%,84.44%)高于对照组(63.83%,48.94%,53.19%)(P<0.05)。Logistic回归分析显示HPV E6/E7mRNA、P16/Ki-67是影响CIN2诊断的危险因素(P<0.05);HPV E6/E7mRNA、P16/Ki-67联合检测CIN2级病变的受试者工作特征曲线下面积为0.688(95%CI:0.579~0.798),优于HPV E6/E7mRNA、P16/Ki-67单独预测[0.592(95%CI:0.476~0.708);0.634(95%CI:0.519~0.748)]。联合检测的准确度、灵敏度、特异度(68.8%,84.4%,53.2%)均高于单独检测HPV E6/E7mRNA(59.2%,82.2%,36.2%)、P16/Ki-67(63.4%,77.8%,48.9%)。结论:HPV E6/E7mRNA与P16/Ki-67免疫组化检测在宫颈CIN2级病变筛查中具有一定的应用价值,可以显著提高筛查宫颈CIN2级病变的诊断效能。

cyclin E、p53、CD44s、CD44v6与早期乳腺癌的相关性及预后预测分析

目的分析肿瘤组织的细胞周期素E(cyclin E)和p53、CD44s、CD44v6蛋白与早期乳腺癌的相关性及预后预测价值。方法选取2018年7月—2020年7月喀什地区第二人民医院收治的80例临床分期为Ⅰ~Ⅱ期的早期乳腺癌患者进行回顾性分析,将其分为恶性组;另选取同期喀什地区第二人民医院收治的80例通过病理学检验确诊为良性乳腺肿瘤患者,将其分为良性组。分别对两组患者留存的肿瘤组织进行免疫组化检测,检测cyclin E、p53、CD44s、CD44v6表达,并对比上述指标的阳性率,应用Spearman相关分析方法分析cyclin E、p53、CD44s、CD44v6与早期乳腺癌的相关性。所有患者均采取手术治疗,随后对80例早期乳腺癌患者进行3年随访,依照随访结果将患者分为2个亚组,即预后不良组(n=25)和预后良好组(n=55),对比两组患者一般临床特征及cyclin E、p53、CD44s、CD44v6阳性率,并应用Logistic回归分析cyclin E、p53、CD44s、CD44v6对早期乳腺癌预后的预测价值。结果恶性组患者cyclin E、p53、CD44s、CD44v6阳性率分别为42.50%、61.25%、77.50%、81.25%,明显高于良性组1.25%、5.00%、10.00%、12.50%(P<0.05);Spearman相关分析结果显示:cyclin E、p53、CD44s、CD44v6与早期乳腺癌呈正相关(P<0.05);预后良好组与预后不良组患者绝经情况、年龄、病理类型、治疗方式、三阴性乳腺癌、肿瘤大小、组织分化程度对比,差异无统计学意义(P>0.05),预后良好组与预后不良组患者临床分期、cyclin E、p53、CD44s、CD44v6阳性例数对比,差异有统计学意义(P<0.05);Logistic回归分析结果表明:cyclin E、p53、CD44s、CD44v6阳性表达为影响早期乳腺癌预后的独立影响因素(P<0.05)。结论cyclin E、p53、CD44s、CD44v6表达水平与乳腺癌的发生、发展具有明显关系,且上述指标呈阳性表达为早期乳腺癌预后不良的独立预测因素。