中国—印尼跨境电子商务领域数字人文交流研究

在“一带一路”倡议的推动下,中国与印度尼西亚的跨境电子商务合作已成为促进两国经济合作和人文交流的重要渠道。文章通过探讨数字人文交流的新内涵及其在跨境电商中的重要作用,构建了中国—印尼跨境电商与人文交流的双向交融的新模式,为跨境电商与人文交流的协同发展提供了新思路,有利于推动双边跨境电商持续繁荣和丰富人文交流的内容内涵,深化两国命运共同体构建发展。

Study on Ethnical Bamboo Culture Ecotourism in Yunnan Province

As a specific region most abundant in bamboo species and their natural forests i n the world, China's Yunnan Province harbors over 250 bamboo species belongin g to 28 genera in its 331 000?hm\+2 of bamboo forests,thus forming a unique macro\| ba ckground of its ecotourist landscape resources.Not only is it acknowledged as on e of the original places and modern distribution centers of bamboo by scientists ,but it is a region with diverse ethnical and bamboo cultures nurtured out of th e peculiar ethnical customs and wide bamboo utilization of its 26 nationalities. Based on the analyses of the ornamental bamboo and its value in landscape resour ces,5 aesthetic characteristics are microscopically and morphologically approach ed as a material base of the formation of bamboo culture.By reexamining the macr o\|background of the specific natural and humanity landscape,4 categories are de scribed as fundamental macro\|landscape resources of Yunnan's bamboo culture eco tourism.After researching the ethnical ecological ethics and cultural symbolism, 3 typical ethnical traditions are summarized as traditional ecological awareness ,and 3 cultural features of bamboo are revealed as essence of ethnical bamboo cu lture preserved in Yunnan.Borrowing some theories from the new economic developm ent,innovation and successful ecotourist management experience at home and abroa d with wide academic vision,the authors have synthetically studied the substanti al,conceptual and symbolic landscapes in a broad field involving bamboo science, ethnology,ecology,ecoeconomics and tourist management.A preliminary theory of e thnical bamboo culture is thus constructed,and its peculiarities are briefly dis cussed while the correlation between ethnical bamboo culture and the natural res ources,other cultures as well as ecotourist development are systematically analy zed to lay a solid theoretical foundation and reliable scientific basis for ecoe conomy\|oriented forestry,cultural economics and ecotourist practice.Accordingly ,3 primary strategies and certain related principles are suggested for the susta inable development of ecotourism in Yunnan via effective protection and rational exploitation of abundant natural landscape resources and colorful humanity land scape resources including ethnical bamboo culture.

miR-221-3p通过NRF2/GPX4轴调节铁死亡促进乳腺癌细胞转移的分子机制研究

目的:探究微小RNA(miR)-221-3p通过调节铁死亡对乳腺癌细胞转移的影响及机制。方法:培养人乳腺癌细胞系MCF-7,采用miR-221-3p模拟物(mimic)及mimic阴性对照(NC)转染MCF-7细胞,实时荧光定量PCR(RT-qPCR)检测转染后细胞中miR-221-3p表达,试剂盒测定转染后细胞中二价铁离子(Fe^(2+))、活性氧(ROS)、还原型谷胱甘肽(GSH)水平。将MCF-7细胞分为对照组、mimic NC组(转染mimic NC)、miR-221-3p mimic组(转染miR-221-3p mimic)、miR-221-3p mimic+Erastin组(转染miR-221-3p mimic并用10μmoL/L Erastin处理),CCK-8检测各组MCF-7细胞增殖活性,Transwell实验检测各组MCF-7细胞迁移数目与侵袭数目,试剂盒测定各组细胞中Fe^(2+)、ROS、GSH水平,细胞免疫荧光双标记染色观察各组MCF-7细胞中核因子E2相关因子2(NRF2)与谷胱甘肽过氧化物酶4(GPX4)表达,蛋白质免疫印记(Western blot)观察各组MCF-7细胞中NRF2、GPX4、溶质载体家族7成员11(SLC7A11)和铁蛋白轻链(FTL)蛋白表达。结果:转染miR-221-3p mimic后,MCF-7细胞中miR-221-3p相对表达量上调(P<0.05),Fe^(2+)含量减少、ROS水平降低(P<0.05),GSH水平升高(P<0.05)。与对照组、mimic NC组比较,miR-221-3p mimic组MCF-7细胞增殖活性升高(P<0.05),迁移数目和侵袭数目增加(P<0.05),Fe^(2+)含量减少、ROS水平降低(P<0.05),GSH水平升高(P<0.05),NRF2和GPX4荧光强度明显增强,NRF2、GPX4、SLC7A11和FTL蛋白相对表达量上调(P<0.05);与miR-221-3p mimic组比较,miR-221-3p mimic+Erastin组MCF-7细胞增殖活性降低(P<0.05),迁移数目和侵袭数目减少(P<0.05),Fe^(2+)含量增加、ROS水平升高且GSH水平降低(P<0.05),NRF2和GPX4荧光强度减弱,NRF2、GPX4、SLC7A11和FTL蛋白相对表达量下调(P<0.05)。结论:miR-221-3p通过抑制铁死亡促进乳腺癌细胞转移,其机制可能与激活NRF2/GPX4轴有关。

从C.P.E.巴赫的音乐中探寻情感的表现

早在18世纪,作曲家C.P.E.巴赫就在他著名的文献《键盘乐器演奏艺术真谛》中阐述了用纯乐器音乐手法来表现情感,这样的音乐被认为是"感情的语言"。作为一名演奏者,我们从他的思想中进一步剖析和拓展,在演奏的同时力求对作品精神、情感全面地展现。

Introduction of Internet P.E News Reporter

Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant with personal information.

Development Characteristics of Private P.E Economy

The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and the overall staff quality,develop P.E vocational P.E to provide P.E talents for private P.E economy.

P.E Science J ournals General Influence Factor

The portal is characterized with large amount of information, high stability and interaction, while "Online Journal of Physical P.E" is to publicize the academic pure value, spread the achievement influence in daily life, and advocate an academic atmosphere of utilitarianism with a slogan of "article issued online can be published in journal with the recommendation of experts" and practical action. It is the first academic journal under network operation.

苏气汤加味治疗肩袖损伤(气滞血瘀证)患者的回顾性研究

目的 回顾性观察苏气汤加味治疗肩袖损伤(RCI)(气滞血瘀证)的临床效果。方法 选取2021年12月至2023年12月于本院治疗的RCI患者82例,根据不同的治疗方法分为对照组与观察组各41例。对照组接受常规康复训练。观察组在对照组基础上予苏气汤加味内服。两组观察2周。统计两组治疗效果、疼痛评分、肩关节功能美国肩肘外科协会评分(ASES)、肩活动度、血清疼痛递质P物质(SP)、前列腺素E2(PGE2)、5-羟色胺(5-HT)水平等相关数据。结果 治疗后,观察组总有效率为97.56%,显著高于对照组的78.05%(P<0.05)。治疗后,两组视觉模拟量表(VAS)评分明显下降,ASES评分明显增加,同时观察组改善更显著(P<0.05)。治疗后,两组前屈、外展、体侧外旋的角度显著增大,同时观察组增大更显著(P<0.05)。治疗后,两组血清SP、PGE2、5-HT水平显著降低,同时观察组降低更显著(P<0.05)。结论 苏气汤加味治疗RCI(气滞血瘀证)的临床效果显著,可明显减轻患者的疼痛,改善肩关节功能,以及下调疼痛递质。

Needs Analysis of English Culture Background Introduction in Spoken English Classes

With globalization there is an increasing need for spoken English for Chinese college students. However, the situation of the teaching system of spoken English in most universities in China is not satisfactory. Influenced by the exam-oriented teaching system, great importance has been put on English grammar and the skills of reading and writing. As a result, students'enthusiasm in improving their spoken English is diminished. Actually, students need some knowledge about English culture background before they can communicate effectively. It is significant for teachers to find out the students'wants and lacks during their spoken English study. Besides, attentions should be paid to English culture background in spoken English classes by both teachers and students. This paper mainly aimed at finding out the needs of students for English culture background in spoken English classes based on the theory of needs analysis.

蒙特威尔第“激动风格”和C.P.E.巴赫“激情风格”之比较

本文选取了文艺复兴晚期和前古典主义时期的两位代表作曲家的创作风格,以作曲家所处的时代背景为依托着重阐述两种不同时代音乐风格的异同,力从对比中着眼看待其对西方音乐发展的影响。

一部永不休止的流动经典——C.P.E巴赫与他的《钢琴弹奏艺术》

C.P.E.巴赫是“北德乐派”的代表作曲家。作为一个伟大的音乐家,他不仅继承和发展了其父约翰·塞巴斯蒂安·巴赫的音乐创作风格,而且教出了象贝多芬这样的一代钢琴大师。作为一个伟大的作曲家,音乐史上已有记载,而他在钢琴教学上的伟大成就至今少有记述。本文着重研究他在钢琴教学上的贡献。

Effects of nitrogen specification and culture method on growth of Enteromorpha prolifera

Eutrophication, which is the enrichment of a water mass with inorganic and organic nutrients that support plant growth, is a key factor in stimulating phytoplankton growth. In this study, we determined the effects of various nitrogen sources, different nitrogen concentrations in the culture medium, and two culture methods on the growth of the green alga, Enteromorpha prolifera. The relationship between the specific growth rate of E. prolifera and NO3-N concentration was consistent with that estimated using the Monod equation （R2=0.9713, P〈0.01）. In the N O3-N medium, the maximum specific growth rate was calculated to be 0.1634/d and the semi-saturation constant was calculated to be 16.86 μmol/L. Our results show that E. prolijkra can effectively utilize NH4^＋-N, NO3^-N, and NO^2-N and urea-N in the range of 5 to 50 μmol/L. NH4^＋-N was preferentially assimilated by E. prolifera, and urea-N was favorable for long-term growth.

<i>N</i>–nitrosodiethylamine cytochrome P450 induction and cytotoxicity evaluation in primary cultures of rat hepatocytes

The primary routes of potential human exposure to N-nitrosodiethylamine (NDEA) are ingestion, inhalation, and dermal contact. Air, diet and smoking contribute to potential human exposure at levels of a few μg of NDEA/day. Potential exposure depends on the ability of the nitrosamines to migrate from the product into the body. The first step in the metabolic degradation of NDEA by cytochrome oxidase (CYPs) enzymes is the introduction of a hydroxyl group and in human esophage and liver CYP2A3 and CYP2E1 participate on this metabolism. Measuring cytotoxicity in female rat primary hepatocytes cultures, were used to understand the CYP induction and metaboli-zation correlated with low NDEA concentrations. We observed that NDEA at different concentrations in the absence of CYPs inducers, was able to induce CYP2B1, CYP2B2, CYP2E1, CYP3A1 and CYP4A3. A positive NDEA synergistic effect on the levels of mRNA, was observed in the presence of pyrazole (300 μM) for CYP2B1 and CYP2B2 and for pregnenolone 16- carbonitrile (0.15 μM) for CYP2E1. Negative NDEA synergistic effects were observed for ethanol (0.3%) for CYP3A1, pyrazol (300 μM) for CYP2A1 and CYP2E1, and phenobarbital (1 mM) for CYP2A1. These facts are extremally important once that these metabolites can be directly related to the primary DNA lesions. We consider that studies to elucidate the biological factors that determine the shape of the dose-response curve are crucial for low-dose extrapolations of risk.

论C.P.E巴赫的三部歌曲集创作

C.P.E巴赫(Carl Philipp Emanuel Bach,1714~1788)上承巴洛克时代下启古典主义风格,无论在古钢琴奏鸣曲还是交响曲等体裁的创作上,都无疑是位举足轻重的人物。他作为柏林歌曲乐派的代表人物,一生创作了近300首德文歌曲,奠定了18世纪德文歌曲写作手法,并推动了19世纪艺术歌曲的发展。以其一生的三部大型歌曲集为叙述重点,论述其德文歌曲创作的几个阶段,分析其歌曲特点,进而阐释与探析18世纪德文歌曲的写作风格。

Sequence analysis of VP4 genes of wild type and culture adapted human rotavirus G1P[8]strains

Objective:To conduct a comparative analysis of the VP4 gene sequences of Indian wild type (06361,0613158,061060 and 0715880) and cell culture adapted(06361-CA,0613158-CA.061060- CA and 0715880-CA) G1P[8]rotavirus strains.Methods:Full-length VP4 genes of each of the four wild type G1P[8]rotavirus strains and their cell culture adapted counterparts displaying consistent cytopathic effect were subjected to RT-PCR amplification and nucleotide sequencing. Results:All four cell culture adapted G1P[8]rotavirus strains showed nucleotide and amino acid substitutions in the VP4 gene as compared to their wild type strains.The number of substitutions however,varied from 1-64 and 1-13 respectively.The substitutions were distributed in both VP5* and VP8* subunits of VP4 gene respectively of permeabilizalion and hemagglutinaling activity. The presence of unique amino acid substitutions was identified in two of the four wild type(V377G. S387N in 061060 and 1644L in 0715880) and all four cell culture adapted(A46V in 0613158-CA. T60R in 06361-CA,L237V.G389V and Q480H in 061060-CA and S615G and T625P in 0715880-CA) strains for the first time in the VP4 gene of P[8]specificity.Amino acid substitutions generated increase in the hydrophilicity in the cell culture adapted rotavirus strains as compared to their corresponding wild type strains.Conclusions:Amino acid substitutions detected in the VP4 genes of G1P[8]rotavirus strains from this study together with those from other studies highlight occurrence of only strain and/or host specific substitutions during cell culture adaptation. Further evaluation of such substitutions for their role in attenuation,immunogenicity and conformation is needed for the development of newer rolavirus vaccines.

Qualitative Study of Three Cell Culture Methods

Primary rat hepatocytes were cultured using different in vitro models and the enzym e leakage,albumin secretion,and cytochrome P4 5 0 1A(CYP1A) activity were observed.The re- sults showed that the level of L DH was decreased over time in culture.However,on day5 ,L DH showed a significant increase in monolayer culture (MC) while after day 8no L DH was detectable in sandwich culture (SC) . The levels of AST and AL T did not change significantly over the inves- tigated time. The CYP 1A activity was gradually decreased in a time- dependent manner in MC and SC.The decline of CYP1A was faster in MC than in SC.This effectwas partially reversed by us- ing cytochrom e P4 5 0 (CYP4 5 0 ) inducer such as Omeprazol and3- m ethylcholanthrene(3- MC) and the CYP1A induction was always higher in MC than in SC.In bioreactor basic CYP1A activity was preserved over2 weeks and the highest album in production was observed in bioreactor fol- lowed by SC and MC. Taken together,it was indicated each investigated model had its advantages and disadvantages. It was also underlined thatvarious in vitro models may address different ques- tions.

THE ULTRASTRUCTURE OF SEPARATED AND CULTURED CELL OF PORPHYRA YEZOENSIS

There are many reports that cells (protoplasts) separated from the thallus of Porphyra by enzyme can develop to normal leafy thalli in the same way as monospores. But there are few investigations on the subcellular structure of the isolated vegetative cell for comparison with the subcellular structure of monospores. To clarify whether the separated and cultured cells undergo the same or similar ultrastructure changes during culture and germination as monospores undergo in their formation and germination, we observed their ultrastructure, compared them with those of the monospore and found that the ultrastructure of separated and cultured cells did not have the characteristic feature as that of monospore formation, such as production of small and large fibrous vesicles, but was accompanied by vacuolation and starch mobilization like that in monospore germination. The paper also discusses the relations between monospores and separated and cultured cells.

Observations on the Contraction and Movement of Fat-Storing Cells in Culture

The Wister fat-storing cells were isolated by perfusion with collagenase and centrifugation with metrizamide density cushion technique and cultured in vitro. The fatstoring cells were confirmed by the presentation of the lipid drop in the cytoplasm and the visualization of dismin with anti-dismin antibody by using indirect immunofluorescence method. By the videotape recorder (VIR) and the imagine analysis system, we observed the wandering immigration, the abrupt contraction of fat-storing cells with spike, then becoming a ball-like shape in its division phase. After that the cell began to extend and the contraction of these cells can be induced by the presence of 10-2 mmol/L endothelin-1 , 1 mmol/L of substance P and 2×10-5 mmol/L noradrenalin. After removal of these agents the contracted cells would become extended. All these findings indicate that the fat-storing cells have ability of contraction and movement.

清肾颗粒对大鼠NRK-52E细胞转分化模型miR-23b和PINK1/Parkin通路的影响

目的 探讨TGF-β1诱导大鼠NRK-52E细胞转分化模型中miR-23b对PINK1/Parkin通路的靶向调节机制,并阐明清肾颗粒含药血清对NRK-52E细胞转分化的干预机理。方法 采用超高效液相色谱(UPLC)指纹图谱法对清肾颗粒进行全指纹图谱分析。构建TGF-β1诱导大鼠NRK-52E细胞转分化模型,转染siRNA后分为模拟物空载对照组、miR-23b-5p模拟物组、抑制剂空载对照组、miR-23b-5p抑制剂组,观察miR-23b-5p对PINK1表达量的影响。再将NRK-52E细胞分组为正常组、TGF-β1组、清肾颗粒组、miR-23b-mimic-NC组、miR-23b-mimic组、miR-23b-mimic+清肾颗粒组,Western blot法检测NRK-52E细胞中Pink1、Parkin、LC3Ⅱ、Beclin-1、P62、α-SMA蛋白表达,RT-qPCR法检测NRK-52E细胞中miR-23b-5p、Pink1、Parkin、Beclin-1、α-SMA mRNA的表达,双荧光素酶报告基因实验检测miR-23b-5p与PINK1的靶向关系。结果 UPLC指纹图谱法鉴定出清肾颗粒中11个活性成分。miR-23b-5p过表达后,PINK1 mRNA表达量也显著增加(P<0.05);而miR-23b-5p表达沉默后,PINK1 mRNA表达量也显著减少(P<0.05)。双荧光素酶报告显示,Rno-miR-23b-5p能显著下调Rno-PINK1-WT荧光素酶活性(P<0.05),但未能下调突变Rno-PINK1-mut荧光素酶活性(P>0.05)。清肾颗粒含药血清干预实验发现,TGF-β1组的miR-23b-5p、Pink1、Parkin、Beclin-1、LC3Ⅱ表达及LC3Ⅱ/Ⅰ比值均明显低于正常组,P62和α-SMA表达明显高于正常组(P<0.05)。清肾颗粒组和miR-23b-mimic组的miR-23b-5p、Pink1、Parkin、Beclin-1、LC3Ⅱ表达及LC3Ⅱ/Ⅰ比值均明显高于TGF-β1组,P62和α-SMA表达明显低于TGF-β1组(P<0.05)。miR-23b-mimic+清肾颗粒组的表现更优于miR-23b-mimic组(P<0.05)。结论 清肾颗粒能够上调NRK-52E细胞内miR-23b-5p表达,并通过增强PINK1/Parkin通路介导的线粒体自噬活性,抑制NRK-52E细胞转分化进程。

Research and Practice of the P.E. Information Teaching Design System

In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database （nine modules in total） on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency.