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尤因塔盆地P.R.泉始新统油砂成藏条件及成藏模式 被引量:3
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作者 单玄龙 张俊锋 罗洪浩 《世界地质》 CAS CSCD 2011年第2期224-230,共7页
尤因塔盆地P.R.泉位于美国犹他州东北部,油砂资源丰富,油砂矿藏面积约650 km2,油砂储量约6.76×108t,储层为始新统绿河组湖泊三角洲相砂体。晚白垩世到古近纪拉拉米运动使盆地由海相转变为陆相盆地。古新世末—始新世,尤因塔湖在盆... 尤因塔盆地P.R.泉位于美国犹他州东北部,油砂资源丰富,油砂矿藏面积约650 km2,油砂储量约6.76×108t,储层为始新统绿河组湖泊三角洲相砂体。晚白垩世到古近纪拉拉米运动使盆地由海相转变为陆相盆地。古新世末—始新世,尤因塔湖在盆地内分布广泛,并沉积了两套巨厚的绿河组烃源岩。其中绿河组上部的中始新统烃源岩厚达150 m,有机质丰富,且仍处于低熟阶段,约30 Ma以来,产生了大量的低熟油。P.R.泉油砂矿藏为斜坡降解型成藏模式,发育5套广布的三角洲砂体,储层物性好。绿河组上部烃源岩生成的低熟油沿着深入烃源岩中的连续砂体经过长距离的运移,进入P.R.泉绿河组三角洲的砂岩储层中,经过生物降解和水洗作用最终形成油砂矿藏。 展开更多
关键词 尤因塔盆地 p.r.泉油砂矿藏 绿河组 低熟油 斜坡降解型
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ABx亚型相关的B型糖基转移酶突变p.R180C的酶动力学研究 被引量:1
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作者 雷航 蔡晓红 +2 位作者 王钰箐 王学锋 邹纬 《中国输血杂志》 CAS 2020年第11期1140-1143,共4页
目的探讨ABx亚型相关的B型糖基转移酶(GTB)突变p.R180C的酶动力学特征。方法以本课题组已构建的野生型GTB-pcDNA3.1真核表达载体为基础,采用定点突变试剂盒构建携带GTB突变p.R180C的表达载体突变体,并以DNA直接测序法验证突变质粒的序列... 目的探讨ABx亚型相关的B型糖基转移酶(GTB)突变p.R180C的酶动力学特征。方法以本课题组已构建的野生型GTB-pcDNA3.1真核表达载体为基础,采用定点突变试剂盒构建携带GTB突变p.R180C的表达载体突变体,并以DNA直接测序法验证突变质粒的序列;以野生型和突变型表达载体转化人胚肾上皮细胞(293F)做蛋白表达;再将经亲和柱Ni-IDA纯化后的表达蛋白以SDS-聚丙酰胺凝胶电泳法鉴定;最后采用酶动力学活性检测试剂盒检测酶动力学参数米氏常数(Km)和催化常数(Kcat)。结果 DNA直接测序:成功构建野生型和突变型GTB-pcDNA3.1真核表达载体;SDS-聚丙酰胺凝胶电泳:野生型和突变型GTB蛋白在293F内成功表达并获得纯化;酶动力学检测:p.R180C突变型GTB的Kcat及Km值分别为野生型GTB的1.7%、3.4倍[岩藻糖(H)受体]与25%、5.2倍[二磷酸尿苷半乳糖(UDP-Gal)供体]。结论 GTB突变p.R180C降低了该酶对H受体和UDP-Gal供体的亲和力和其催化速率,尤其是对H受体的催化速率,进而导致了红细胞表面H抗原生成B抗原的含量减少。 展开更多
关键词 ABO亚型 B型糖基转移酶 突变p.r180C 酶动力学 真核表达
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飞利浦加速器P.R.F异常切换故障的排除
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作者 钱安 《现代医学仪器与应用》 2000年第1期36-37,共2页
故障现象 常规治疗过程中,剂量率突然变低,在MCC上观察P.R.F由200Hz变为50Hz。并且钥匙开关处于“test”位时,P.R.F各挡出光正常;处于“treat位时,无论P.R.
关键词 飞利浦加速器 p.r.F 异常切换故障 排除
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致死性侏儒症p.R248C突变热点的快速检测和三例TD-Ⅰ型高危胎儿的快速产前诊断 被引量:3
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作者 姜煜 潘敬新 +5 位作者 郭东炜 艾阳 李荣 蒋玮莹 方群 郭奕斌 《分子诊断与治疗杂志》 2017年第4期228-233,共6页
目的针对致死性侏儒症I型(thanatophoric dysplasia typeⅠ,TD-Ⅰ)FGFR3基因的突变热点"p.R248C",建立快速特异的酶切鉴定法(restriction endonuelease testing,RE)和扩增受阻突变系统(amplification refractory mutation sys... 目的针对致死性侏儒症I型(thanatophoric dysplasia typeⅠ,TD-Ⅰ)FGFR3基因的突变热点"p.R248C",建立快速特异的酶切鉴定法(restriction endonuelease testing,RE)和扩增受阻突变系统(amplification refractory mutation system,ARMS)/RE法,并应用于后续3例疑似TD-I高危胎儿的快速产前诊断,以及时防止患胎出生,同时为今后的胚胎植入前遗传学诊断(preimplantation genetic diagnosis,PGD)打下良好基础。方法首先,针对突变热点p.R248C突变前后的序列特点,选择合适的内切酶"Afe I",建立酶切鉴定法。其次,设计双错配碱基特异引物结合Apa LI酶切,建立ARMS/RE双重特异鉴定法。对阴性和阳性结果均再用普通引物扩、测的结果进行验证。结果用Afe I酶切FGFR3基因exons(6-7)普通引物的PCR产物(535 bp),正常对照及非p.R248C突变的TD病例均能被切成255 bp和280 bp 2个片段,而胎1~胎3均切出255 bp、280 bp和535 bp 3个片段。用特异引物E7(p.R248C)扩增,正常对照和非p.R248C突变的TD病例均扩增阴性,无法进一步做酶切鉴定;而p.R248C突变均扩增阳性,当再用Apa LI酶切PCR产物(365 bp)时,胎1~胎3均切出22 bp和343 bp 2个片段。通过引物扩、测结果显示:胎1~胎3均是p.R248C杂合突变。结论该法快速特异、准确可靠,可用于p.R248C突变热点的快速检测及含该突变高危胎儿的快速产前诊断。该法还可用于含p.R248C突变TD-I型家系的PGD。胎1~胎3都是TD-I患胎,建议尽早终止妊娠。 展开更多
关键词 致死性侏儒症I型 FGFR3基因 p.r248C突变 ARMS/RE法 快速检测 产前基因诊断
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DEGRADATION OF LINEAR ALKYLABENZENE SULFONATE(LAS)AND ITS COMPOUNDS IN DONGHU LAKE(HUBEI,P.R.C.)DETERMINED BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY(HPLC)
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作者 Ayfer Yediler 徐盈 +1 位作者 张甬元 陈军建 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 1990年第2期188-196,共9页
Commercial linear alkylbenzene sulfonate (LAS), mixture of alkylchain lengths and phenyl position isomers (C10-C13), is widely used as a major constituent of household and industrial detergents in the People’s Republ... Commercial linear alkylbenzene sulfonate (LAS), mixture of alkylchain lengths and phenyl position isomers (C10-C13), is widely used as a major constituent of household and industrial detergents in the People’s Republic of China. Degradation process and behaviour of LAS compounds during an 82-hour lake water die-away study, with an added LAS concentration of 1. 5mg .L-1, was quantified and accomplished by HPLO-UV after extractionon the SepPek C18 reversed - phase cartridges. The degradation rate became progressively faster with increasing chain length. The technique described in this study is fast, sensitive and specific, and can be used to determine low levels, of LAS and for establishing water quality criteria and standards relating to LAS and its compounds. 展开更多
关键词 establishing faster ISOMERS progressively HPLC HUBEI LAS p.r SULFONATE behaviour
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The 5^(th) World Congress on Intelligent Control and Automation(WCICA′04) June,2004,Hangzhou,P.R.China Call for Papers
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《自动化学报》 EI CSCD 北大核心 2003年第4期615-627,共2页
关键词 World Congress on Intelligent Control and Automation for June 2004 Hangzhou p.r.China Call for Papers The 5 WCICA on
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Analysis of the Characteristics of the D.P.R.Korean Seismic Event on September 23,2017
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作者 Du Guangbao Zhao Yong +2 位作者 Sun Li He Shaolin Liu Jie 《Earthquake Research in China》 CSCD 2018年第2期280-287,共8页
A M3. 4 seismic event occurred in the Punggye-ri nuclear test site,D. P. R Korea at 16∶29 p.m. on September 23,2017. We analyzed the waveforms of this event recorded by the China Earthquake Networks Center. In conclu... A M3. 4 seismic event occurred in the Punggye-ri nuclear test site,D. P. R Korea at 16∶29 p.m. on September 23,2017. We analyzed the waveforms of this event recorded by the China Earthquake Networks Center. In conclusion,this event is complex,including an explosion with a successive earthquake. Although the beginning of the event is an explosion,fault dislocation plays the main role in the event. 展开更多
关键词 D.p.r Korea NUCLEAR TEST Explosion FAULT DISLOCATION
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Newly Approved National Standards of P.R.China No.6,2019
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《China Standardization》 2019年第4期I0002-I0015,共14页
State Administration for Market Regulation and Standardization Administration of China have approved the following 173 national standards and 3 national standards with amendment.
关键词 NEWLY Approved NATIONAL STANDARDS p.r.China No.6 2019
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Newly Approved National Standards of P.R.China No.7,2019
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《China Standardization》 2019年第4期I0016-I0046,共31页
State Administration for Market Regulation and Standardization Administration of China have approved the following 374 national standards and 3 national standards with amendment.
关键词 NEWLY Approved NATIONAL STANDARDS p.r.China No.7 2019
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Congratulatory Letter to WFAS from Wu Yi the Vice-Premier of State Council of P.R.C.
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作者 WFAS Secretariat 《World Journal of Acupuncture-Moxibustion》 2007年第4期21-21,共1页
October 19,2007 Vice Premier Wu Yi,PRC On behalf of Chinese government,I would like to congratulate the 20th Foundation of World Federation of Acupuncture-Moxibustion Societies(WFAS)and International Acupuncture Congr... October 19,2007 Vice Premier Wu Yi,PRC On behalf of Chinese government,I would like to congratulate the 20th Foundation of World Federation of Acupuncture-Moxibustion Societies(WFAS)and International Acupuncture Congress which is to be held in Beijing,China.And also I give my warm welcome and regards to TCM/Acupuncture colleagues all over the world. 展开更多
关键词 Congratulatory Letter to WFAS from Wu Yi the Vice-Premier of State Council of p.r.C
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Announcement of Newly Approved National Standards of P.R.China 2010 No.3(total No.158)
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《China Standardization》 2011年第1期62-70,共9页
General Administration of Quality Supervision,Inspection and Quarantine of P.R.China and Standardization Administration of China have approved the following 163 national standards and 81 national reference materials.
关键词 2010 GB CRM Announcement of Newly Approved National Standards of p.r.China 2010 No.3 total No.158
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Announcement of Newly Approved National Standards of P.R.China 2009 No.4 (total No.144)
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《China Standardization》 2010年第1期48-81,共34页
General Administration of Quality Supervision, Inspection and Quarantine of P.R. China and Standardization Administration of China and Standardization Administration of China have approved the following 314 national s... General Administration of Quality Supervision, Inspection and Quarantine of P.R. China and Standardization Administration of China and Standardization Administration of China have approved the following 314 national standards. 展开更多
关键词 GB total No.144 Announcement of Newly Approved National Standards of p.r.China 2009 No.4
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Power Grid Development in 60 Years since the Foundation of P.R.C.
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作者 Sun Ke,Han Feng State Power Economic Research Institute 《Electricity》 2009年第3期17-22,共6页
Foreword Since the foundation of P.R.C.,the China's power grid develops with a high rate.By now,grids in all provinces except Xinjiang,Tibet,Hainan and Taiwan are AC/DC interconnected together,forming the structur... Foreword Since the foundation of P.R.C.,the China's power grid develops with a high rate.By now,grids in all provinces except Xinjiang,Tibet,Hainan and Taiwan are AC/DC interconnected together,forming the structure of power grid interconnection in the whole country. 展开更多
关键词 Power Grid Development in 60 Years since the Foundation of p.r.C UHVDC
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National Verification Regulations and Calibration Specifications of Measuring Instruments of P.R.China in 2009
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《China Standardization》 2009年第4期69-69,共1页
关键词 JJG National Verification Regulations and Calibration Specifications of Measuring Instruments of p.r.China in 2009
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National Verification Regulations and Calibration Specifications of Measuring Instruments of P.R.China in 2010(2010 No.131)
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《China Standardization》 2011年第1期80-80,共1页
General Administration of Quality Supervision,Inspection and Quarantine of P.R.China has approved the following 8 national measuring verification regulations in 2010 and publicize now.
关键词 National Verification Regulations and Calibration Specifications of Measuring Instruments of p.r.China in 2010 No.131
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PICMG/P.R.C年会召开在即 ATCA成关注焦点
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作者 叶惠 《通讯世界》 2006年第3期60-61,共2页
2006年的PICMG/P.R.C年会召开在即,ATCA的阵容无疑将会十分庞大,那么什么才是今年ATCA的主旋律呢?是标准。
关键词 ATCA 英特尔 PICMG/p.r.C 摩托罗拉 运营商 电信设备 电信行业
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P.R.D复合地基设计
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作者 王康乐 叶军 刘洋 《山西建筑》 2015年第6期90-91,共2页
以韩国海云台LCT综合体项目P.R.D(Percussion Rotary Drilling)复合地基为背景,介绍了P.R.D复合地基的承载机理,并从设计原则、设计参数选取、承载力计算等方面阐述了具体的设计方法,为国内类似工程的地基处理提供参考借鉴。
关键词 p.r.D复合地基 设计 承载力 计算
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Announcement of Newly Approved National Standards of P.R.China
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《China Standardization》 2007年第4期46-54,共9页
  General Administration of Quality Supervision, Inspection and Quarantine of P.R. China and Standardization Administration of China have approved the following 114 national standards and publicize now.……
关键词 Announcement of Newly Approved National Standards of p.r.China
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China Rare Earth: The Brilliant Fifty Years──Dedicated to the 50th Anniversary of P.R.China
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《China Rare Earth Information》 1999年第5期1-4,共4页
关键词 China Rare Earth Dedicated to the 50th Anniversary of p.r.China The Brilliant Fifty Years
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Announcement of Newly Approved National Standards of P.R.China 2008 No.3(total No.116)
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《China Standardization》 2008年第3期46-52,共7页
General Administration of Quality Supervision,Inspection and Quarantine of P.R.China and Standardization Administration of China have approved the following 121 national standards and 34 reference materials publicize ... General Administration of Quality Supervision,Inspection and Quarantine of P.R.China and Standardization Administration of China have approved the following 121 national standards and 34 reference materials publicize now. 展开更多
关键词 total No.116 GB Announcement of Newly Approved National Standards of p.r.China 2008 No.3
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