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Survivin和p27^(kipl)在胆囊癌组织中的表达及相关性研究 被引量:4
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作者 张宗利 柴杰 +1 位作者 马德民 高延超 《中国现代普通外科进展》 CAS 2007年第5期401-404,共4页
目的:检测胆囊癌组织中Survivin和p27kipl的表达,研究二者与胆囊癌临床病理特征的关系,并探讨二者的相关性。方法:采用免疫组织化学技术检测50例胆囊癌、20例胆囊息肉、20例胆囊炎组织中Survivin和p27kipl表达。结果:50例胆囊癌组织中39... 目的:检测胆囊癌组织中Survivin和p27kipl的表达,研究二者与胆囊癌临床病理特征的关系,并探讨二者的相关性。方法:采用免疫组织化学技术检测50例胆囊癌、20例胆囊息肉、20例胆囊炎组织中Survivin和p27kipl表达。结果:50例胆囊癌组织中39例Survivin蛋白表达阳性,22例p27kipl蛋白表达阳性,二者表达呈负相关系;Survivin在胆囊息肉和胆囊炎组织中不表达,p27kipl在胆囊息肉和胆囊炎组织中呈高表达;Survivin在胆囊癌组织中的表达与患者性别、年龄、病理分级、Nevin分期和淋巴结转移无关(P>0.05),p27kipl在胆囊癌组织中的表达与病理分级、Nevin分期、淋巴结转移有关(P<0.05),而与年龄、性别无明显相关性。结论:Survivin和p27kipl表达异常可能在胆囊癌的发生、发展中起重要作用,二者在胆囊癌的发生、发展中有相互作用;Survivin在胆囊癌组织中的高表达提示其可能与肿瘤的发生有关;p27kipl有可能成为判断胆囊癌预后的有效指标之一。 展开更多
关键词 胆囊肿瘤·p27^kipl·Survivin·免疫组织化学
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Cyclin D1与P27在口腔鳞癌的表达及意义 被引量:1
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作者 张蓓 《医学理论与实践》 2013年第9期1193-1194,共2页
目的:探讨Cyclin D1与P27在口腔鳞癌的表达及意义。方法:收集64例经病理证实均为口腔鳞状细胞癌,并且临床病理资料完整的病例。用免疫组化方法检测其与正常黏膜的Cyclin D1与P27的表达差异。结果:Cyclin D1在鳞状细胞癌中的表达明显高... 目的:探讨Cyclin D1与P27在口腔鳞癌的表达及意义。方法:收集64例经病理证实均为口腔鳞状细胞癌,并且临床病理资料完整的病例。用免疫组化方法检测其与正常黏膜的Cyclin D1与P27的表达差异。结果:Cyclin D1在鳞状细胞癌中的表达明显高于正常黏膜组织,差异有显著性(P<0.01)。P27在鳞状细胞癌中的表达明显低于正常黏膜组织,差异有显著性(P<0.01)。结论:Cyclin D1过表达可能是口腔鳞状细胞癌发生的早期因素,Cyclin D1通过促进细胞增殖、抑制P27等途径促进肿瘤生成。 展开更多
关键词 口腔鳞癌 CYCLIN D1 p27免疫组织化学
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Expression and hypermethylation of p27^(kip1) in hepatocarcinogenesis 被引量:8
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作者 Pu-Ping Lei Zong-Ji Zhang +3 位作者 Li-Juan Shen Jin-Yun Li Qiong Zou Hua-Xian Zhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第29期4587-4591,共5页
AIM: To study the expressions of p27^kip1 protein and p27mRNA, the hypermethylation of p27^kip1 and the relation between them in various stages of hepatocarcinogenesis. METHODS: p27 protein and p27mRNA were detected... AIM: To study the expressions of p27^kip1 protein and p27mRNA, the hypermethylation of p27^kip1 and the relation between them in various stages of hepatocarcinogenesis. METHODS: p27 protein and p27mRNA were detected by immunohistochemical staining and in situ hybridization respectively in 68 cases of normal liver, liver cirrhosis, pericancerous cirrhosis and hepatocellular carcinoma (HCC). The hypermethylation of p27^kip1 was detected by methylation-specific PCR (MSP) in 44 cases of normal liver, liver cirrhosis, and HCC. RESULTS: The positive rate of p27 protein was 66.7% (4/6) in normal liver, 60.0% (6/10) in liver cirrhosis, 50.0% (12/24) in pericancerous cirrhosis and 21.4% (6/28) in HCC. There were no statistical differences in normal liver, liver cirrhosis and pericancerous cirrhosis, but the positive rate of p27 protein significantly decreased in HCC compared to that in the other groups (P = 0.006, %2 = 7.664). The positive rate of p27^kip1 mRNA was 83.3% (5/6) in normal liver, 70.0% (7/10) in liver cirrhosis, 75.0% (18/24) in pericancerous cirrhosis and 25.0% (7/28) in HCC. There were no statistical differences in normal liver, liver cirrhosis and pericancerous cirrhosis, but the positive rate of p27^kip1 mRNA also significantly decreased in HCC compared to that in the other groups (P = 0.000, %2 = 16.600). In addition, there was a significant correlation between the expression of p27 protein and p27mRNA in the integrated group of normal liver and liver cirrhosis. However, no significant correlation was found between pericancerous cirrhosis and HCC. Using MSP, we found that 1 HCC in 44 cases (including 6 cases of normal liver, 10 cases of liver cirrhosis and 28 cases of HCC) was methylated, whose p27 protein and p27mRNA were negative. CONCLUSION: The reduction or loss of p27 protein and p27mRNA are potentially involved in hepatocarcinogenesis. The hypermethylation of p27 might lead to the loss of p27mRNA transcription. 展开更多
关键词 Hepatocellular carcinoma p27^KIp1 Immunohistochemical staining In situ hybridization HYpERMETHYLATION
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