High mycoplasmal infection ratio in gastric cancer tissues suggests a possible association between my-coplasma infection and tumorigenesis. Because TNF-a plays an important role in carcinogenesis caused by microbes in...High mycoplasmal infection ratio in gastric cancer tissues suggests a possible association between my-coplasma infection and tumorigenesis. Because TNF-a plays an important role in carcinogenesis caused by microbes in-fection and P37 is a major immunogen of mycoplasma hy-orhinis (M. hyor.), investigating whether P37 could induceexpression and secretion of TNF-a will be very significant to elucidate the possible molecular mechanism of gastric car-cinogenesis involved with M. hyor. At the present study, we cloned full gene of p37 by PCR and mutated the 7 codes of TGA into TGG firstly, then expressed the P37 protein suc-cessfully with pGEX-4T-1 vector in E. coli, which was veri-fied with Western blot. By RT-PCR and sensitive L929 cell toxic assay, we found that P37 protein could induce expres-sion and secretion of TNF-a from human peripheral bloodmononuclear cells, and the inducing activity of P37 could be dramatically blocked by McAb PD4. These results suggestthat the induction of TNF-a secretion by P37 probably plays an important role in diseases caused by M. hyor. infectionand needs to be further investigated.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.30130190)Beijing Natural Science Foudation(Grant No.7012007)and Cancer Center of Peking University.
文摘High mycoplasmal infection ratio in gastric cancer tissues suggests a possible association between my-coplasma infection and tumorigenesis. Because TNF-a plays an important role in carcinogenesis caused by microbes in-fection and P37 is a major immunogen of mycoplasma hy-orhinis (M. hyor.), investigating whether P37 could induceexpression and secretion of TNF-a will be very significant to elucidate the possible molecular mechanism of gastric car-cinogenesis involved with M. hyor. At the present study, we cloned full gene of p37 by PCR and mutated the 7 codes of TGA into TGG firstly, then expressed the P37 protein suc-cessfully with pGEX-4T-1 vector in E. coli, which was veri-fied with Western blot. By RT-PCR and sensitive L929 cell toxic assay, we found that P37 protein could induce expres-sion and secretion of TNF-a from human peripheral bloodmononuclear cells, and the inducing activity of P37 could be dramatically blocked by McAb PD4. These results suggestthat the induction of TNF-a secretion by P37 probably plays an important role in diseases caused by M. hyor. infectionand needs to be further investigated.
