The effects of exogenous p16^ink4a gene on biological behaviors of human lung cancer cell line with homozygous deletion of p16^ink4a gene were investigated. Exogenous p16^ink4a gene was transfected by lipofectin into ...The effects of exogenous p16^ink4a gene on biological behaviors of human lung cancer cell line with homozygous deletion of p16^ink4a gene were investigated. Exogenous p16^ink4a gene was transfected by lipofectin into human lung cell line A549, in which p16^ink4a gene was homozygously deleted. The expression of p16^ink4a mRNA and protein was detected by RT-PCR and immunocytochemistry, respectively. The changes in the behaviors of the transfected cell lines in vitro and in vivo were observed. In the transfected cell line A549, the exogenous p16^ink4a gene could be stably expressed. The growth of A549 cells transfected with p16^ink4a gene was obviously slowed down. Flow cytometry revealed that transfection of the exogenous p16^ink4a gene resulted in A549 cell lines arrest in G1 phase of cell cycle. The tumorigenicity of these transfected cells in nude mice could be inhibited, and the tumor growth of nude mice was significantly suppressed. It was concluded that exogenous p16^ink4a gene may be stably expressed in human lung cancer cell line A549. The expression of the introduced p16^ink4a could block lung cancer cells to entry into S phase of cell cycle and inhibit tumor malignant growth both in vitro and in vivo.展开更多
Objective To identify the association of thyroid stimulating hormone receptor(TSHR)gene intron 1 susceptible loci and 4p14 susceptible locus rs6832151 polymorphisms with Graves’disease(GD)in Han Chinese population in...Objective To identify the association of thyroid stimulating hormone receptor(TSHR)gene intron 1 susceptible loci and 4p14 susceptible locus rs6832151 polymorphisms with Graves’disease(GD)in Han Chinese population in Bengbu,Anhui,China.The gene-gene interaction among TSHR intron 1 susceptible loci and 4p14susceptible locus rs6832151 was also investigated.Methods The genotypes of the single-nucleotide polymor-展开更多
基金This project was supported by a grant from the Science Research Foundation of Hubei Province, China (No. 98J102).
文摘The effects of exogenous p16^ink4a gene on biological behaviors of human lung cancer cell line with homozygous deletion of p16^ink4a gene were investigated. Exogenous p16^ink4a gene was transfected by lipofectin into human lung cell line A549, in which p16^ink4a gene was homozygously deleted. The expression of p16^ink4a mRNA and protein was detected by RT-PCR and immunocytochemistry, respectively. The changes in the behaviors of the transfected cell lines in vitro and in vivo were observed. In the transfected cell line A549, the exogenous p16^ink4a gene could be stably expressed. The growth of A549 cells transfected with p16^ink4a gene was obviously slowed down. Flow cytometry revealed that transfection of the exogenous p16^ink4a gene resulted in A549 cell lines arrest in G1 phase of cell cycle. The tumorigenicity of these transfected cells in nude mice could be inhibited, and the tumor growth of nude mice was significantly suppressed. It was concluded that exogenous p16^ink4a gene may be stably expressed in human lung cancer cell line A549. The expression of the introduced p16^ink4a could block lung cancer cells to entry into S phase of cell cycle and inhibit tumor malignant growth both in vitro and in vivo.
文摘Objective To identify the association of thyroid stimulating hormone receptor(TSHR)gene intron 1 susceptible loci and 4p14 susceptible locus rs6832151 polymorphisms with Graves’disease(GD)in Han Chinese population in Bengbu,Anhui,China.The gene-gene interaction among TSHR intron 1 susceptible loci and 4p14susceptible locus rs6832151 was also investigated.Methods The genotypes of the single-nucleotide polymor-
