AIM:To evaluate the association between p53 codon 72 polymorphism and liver cancer risk by means of meta-analysis. METHODS:Two investigators independently searched the Medline,Embase and Chinese Biomedicine databases....AIM:To evaluate the association between p53 codon 72 polymorphism and liver cancer risk by means of meta-analysis. METHODS:Two investigators independently searched the Medline,Embase and Chinese Biomedicine databases.Summary odds ratios and 95%CI for p53 codon 72 polymorphism and liver cancer were calculated in fixedeffects model(Mantel-Haenszel method)and randomeffects model(DerSimonian and Laird method)when appropriate. RESULTS:This meta-analysis included 1115 liver cancer cases and 1778 controls.The combined results based on all studies showed that there was a statistically significant link between Pro/Pro genotype and liver cancer,but not between Arg/Arg or Pro/Arg genotype and liver cancer.When stratifying for race,similar results were obtained,i.e.patients with liver cancer had a significantly higher frequency of Pro/Pro genotype than non-cancer patients among Asians.After stratifying thevarious studies by control source,gender,family history of liver cancer and chronic hepatitis virus infection,we found that(1)patients among hospital-based studies had a significantly higher frequency of Pro/Pro and a significantly lower frequency of Arg/Arg genotype than individuals without cancer;(2)female patients with liver cancer had a significantly lower frequency of Arg/Arg and a higher frequency of Pro/Arg+Pro/Pro genotypes than female individuals without cancer;(3)subgroup analyses for family history of liver cancer did not reveal any significant association between p53 codon 72 polymorphism and liver cancer development;and(4) patients with negative hepatitis virus infection had a significantly higher frequency of Pro/Pro and a significantly lower frequency of Arg/Arg genotype than individuals without cancer. CONCLUSION:This meta-analysis suggests that the p53 codon 72 polymorphism may be associated with liver cancer among Asians.展开更多
AIM： To evaluate the potential association between p53 codon 72 polymorphism and sporadic colorectal adenocarcinoma development, and human papillornavirus （HPV） infection. METHODS： One-hundred and nine controls an...AIM： To evaluate the potential association between p53 codon 72 polymorphism and sporadic colorectal adenocarcinoma development, and human papillornavirus （HPV） infection. METHODS： One-hundred and nine controls and 53 patients with colon cancer from the city of La Plata, Argentina were analyzed, p53 codon 72 genotypes and HPV infection were identified using allele-specific polymerase chain reaction and nested polymerase chain reaction, respectively. RESULTS： The differences in the distribution ofp53 codon 72 polymorphisrn between the cases and controls were statistically significant. The arginine allele had a prevalence of 0.65 in controls and 0.77 in cases. The corresponding odds ratio for the homozygous arginine genotype was 2.08 （95% CI, 1.06-4.05; P〈0.05）. Lack of association was found between ,053 polymorphism and HPV infection in the set of adenocarcinomas. CONCLUSION： The findings of the present study indicate that p53 codon 72 arginine homozygous genotype may represent a genetic predisposing factor for colon cancer development. However, further studies are needed in order to elucidate the role of p53 codon 72 polymorphism in colorectal cancer.展开更多
The authors examined 184 residents from Kazakhstan to reveal an association of the CCND1 gene A870G and TP53 gene Arg72Pro polymorphisms with esophageal cancer risk. 86 of them were control group and 98 were patients ...The authors examined 184 residents from Kazakhstan to reveal an association of the CCND1 gene A870G and TP53 gene Arg72Pro polymorphisms with esophageal cancer risk. 86 of them were control group and 98 were patients with esophageal cancer. DNA samples were genotyped by direct sequencing method and TaqMan allelic discrimination method. Statistical analysis was performed using GraphPad InStatTM Software and ＂Case-Control Study Estimating Calculator＂ from TAPOTILI company. A significant association was revealed between CCND1 homozygous genotype （A870A, OR=2.654） and TP53 heterozygous （Arg72Pro, OR= 1.417） and homozygous （Pro72 Pro, OR=2.860） genotypes increased risk of esophageal cancer.展开更多
AIM:To investigate the potential role of p53 codon 72 polymorphism as a risk factor for development of anal cancer. METHODS:Thirty-two patients with invasive anal carcinoma and 103 healthy blood donors were included i...AIM:To investigate the potential role of p53 codon 72 polymorphism as a risk factor for development of anal cancer. METHODS:Thirty-two patients with invasive anal carcinoma and 103 healthy blood donors were included in the study.p53 codon 72 polymorphism was analyzed in blood samples through polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing. RESULTS:The relative frequency of each allele was 0.60 for Arg and 0.40 for Pro in patients with anal cancer, and 0.61 for Arg and 0.39 for Pro in normal controls. No significant differences in distribution of the codon 72 genotypes between patients and controls were found. CONCLUSION:These results do not support a role for the p53 codon 72 polymorphism in anal carcinogenesis.展开更多
By southern hybridization with 1. 8 kb cDNA probe,a high frequency （40. 5 % ） of structural abnor- mality of p53 gene was observed in primary nasopharyngeal carcinoma （NPC） biopsies. The regions of ex- ons 1 to 4 ...By southern hybridization with 1. 8 kb cDNA probe,a high frequency （40. 5 % ） of structural abnor- mality of p53 gene was observed in primary nasopharyngeal carcinoma （NPC） biopsies. The regions of ex- ons 1 to 4 of the gene were examined by polymerase chain reaction-single strand con formation polymor- phism,no point mutation was found. Because very low rate of point mutation had been reported in exons 5 to 8, we considered that structural abnormality in the region of e-cons 1 to 8 of the gene might be uncom- mon in NPC. The spectrophotometer scanning analysis of autoradiograms and rehybridization investigation of nitrocellulose filter with exon 11 probe indicated that most of structure aberrations we observed might be rearrangement occurring in exon 11.展开更多
文摘AIM:To evaluate the association between p53 codon 72 polymorphism and liver cancer risk by means of meta-analysis. METHODS:Two investigators independently searched the Medline,Embase and Chinese Biomedicine databases.Summary odds ratios and 95%CI for p53 codon 72 polymorphism and liver cancer were calculated in fixedeffects model(Mantel-Haenszel method)and randomeffects model(DerSimonian and Laird method)when appropriate. RESULTS:This meta-analysis included 1115 liver cancer cases and 1778 controls.The combined results based on all studies showed that there was a statistically significant link between Pro/Pro genotype and liver cancer,but not between Arg/Arg or Pro/Arg genotype and liver cancer.When stratifying for race,similar results were obtained,i.e.patients with liver cancer had a significantly higher frequency of Pro/Pro genotype than non-cancer patients among Asians.After stratifying thevarious studies by control source,gender,family history of liver cancer and chronic hepatitis virus infection,we found that(1)patients among hospital-based studies had a significantly higher frequency of Pro/Pro and a significantly lower frequency of Arg/Arg genotype than individuals without cancer;(2)female patients with liver cancer had a significantly lower frequency of Arg/Arg and a higher frequency of Pro/Arg+Pro/Pro genotypes than female individuals without cancer;(3)subgroup analyses for family history of liver cancer did not reveal any significant association between p53 codon 72 polymorphism and liver cancer development;and(4) patients with negative hepatitis virus infection had a significantly higher frequency of Pro/Pro and a significantly lower frequency of Arg/Arg genotype than individuals without cancer. CONCLUSION:This meta-analysis suggests that the p53 codon 72 polymorphism may be associated with liver cancer among Asians.
基金Supported by the National University of La Plata (grant v-138), Argentina
文摘AIM： To evaluate the potential association between p53 codon 72 polymorphism and sporadic colorectal adenocarcinoma development, and human papillornavirus （HPV） infection. METHODS： One-hundred and nine controls and 53 patients with colon cancer from the city of La Plata, Argentina were analyzed, p53 codon 72 genotypes and HPV infection were identified using allele-specific polymerase chain reaction and nested polymerase chain reaction, respectively. RESULTS： The differences in the distribution ofp53 codon 72 polymorphisrn between the cases and controls were statistically significant. The arginine allele had a prevalence of 0.65 in controls and 0.77 in cases. The corresponding odds ratio for the homozygous arginine genotype was 2.08 （95% CI, 1.06-4.05; P〈0.05）. Lack of association was found between ,053 polymorphism and HPV infection in the set of adenocarcinomas. CONCLUSION： The findings of the present study indicate that p53 codon 72 arginine homozygous genotype may represent a genetic predisposing factor for colon cancer development. However, further studies are needed in order to elucidate the role of p53 codon 72 polymorphism in colorectal cancer.
文摘The authors examined 184 residents from Kazakhstan to reveal an association of the CCND1 gene A870G and TP53 gene Arg72Pro polymorphisms with esophageal cancer risk. 86 of them were control group and 98 were patients with esophageal cancer. DNA samples were genotyped by direct sequencing method and TaqMan allelic discrimination method. Statistical analysis was performed using GraphPad InStatTM Software and ＂Case-Control Study Estimating Calculator＂ from TAPOTILI company. A significant association was revealed between CCND1 homozygous genotype （A870A, OR=2.654） and TP53 heterozygous （Arg72Pro, OR= 1.417） and homozygous （Pro72 Pro, OR=2.860） genotypes increased risk of esophageal cancer.
基金Supported by The National Council for Scientific and Technological Development(CNPq),No.142678/2007-4,Brazilian Government
文摘AIM:To investigate the potential role of p53 codon 72 polymorphism as a risk factor for development of anal cancer. METHODS:Thirty-two patients with invasive anal carcinoma and 103 healthy blood donors were included in the study.p53 codon 72 polymorphism was analyzed in blood samples through polymerase chain reaction-restriction fragment length polymorphism and DNA sequencing. RESULTS:The relative frequency of each allele was 0.60 for Arg and 0.40 for Pro in patients with anal cancer, and 0.61 for Arg and 0.39 for Pro in normal controls. No significant differences in distribution of the codon 72 genotypes between patients and controls were found. CONCLUSION:These results do not support a role for the p53 codon 72 polymorphism in anal carcinogenesis.
文摘By southern hybridization with 1. 8 kb cDNA probe,a high frequency （40. 5 % ） of structural abnor- mality of p53 gene was observed in primary nasopharyngeal carcinoma （NPC） biopsies. The regions of ex- ons 1 to 4 of the gene were examined by polymerase chain reaction-single strand con formation polymor- phism,no point mutation was found. Because very low rate of point mutation had been reported in exons 5 to 8, we considered that structural abnormality in the region of e-cons 1 to 8 of the gene might be uncom- mon in NPC. The spectrophotometer scanning analysis of autoradiograms and rehybridization investigation of nitrocellulose filter with exon 11 probe indicated that most of structure aberrations we observed might be rearrangement occurring in exon 11.
