消癌解毒方及醇提物对肝癌SMMC-7721细胞增殖及P53-P21基因表达的影响

目的探讨消癌解毒方及醇提物对肝癌SMMC-7721细胞增殖及P53、P21基因表达的影响。方法体外培养肝癌细胞,分为肝癌对照组、消癌解毒方组和醇提物组,不同剂量药物分别作用于肝癌细胞24 h、48 h和72 h后,CCK-8法检测各组细胞增殖活性,数码倒置显微镜观察细胞形态,实时定量PCR检测P53和P21基因表达情况。结果作用剂量上,22μg/mL消癌解毒方组、100μg/mL和150μg/mL醇提物组对细胞增殖的抑制作用优于其它剂量组;作用时间上,72 h抑制细胞增殖作用优于48 h和24 h;22μg/mL消癌解毒方组和100μg/mL醇提取物组对细胞形态影响显著;且明显提高P53和P21基因的表达。结论消癌解毒方及醇提物通过上调肝癌SMMC-7721细胞P53基因的表达,并激活其下游P21基因的表达,从而抑制肝癌细胞增殖可能是其抗肿瘤作用机理;而醇提物可能是该方发挥药效的有效作用部位。

Human Papillomavirus Genotypes and Expression of p53 and p21^(WAF1) in Condyloma Acuminatum

This study investigated the relationship between human papillomavirus (HPV) genotype and expression of p53and p21^(WAF1) Expression of p53 and p2l^(WAF1) in 35 cases ofcondyloma acuminatum specimens infected by HPV6/11andHPV16/18 were studied using immunohistochemical staining.All specimens of the condyloma acuminatum case were positivefor expression of p53 and p21^(WAF1). The expression of p53 incondyloma acuminatum infected by HPV16/18 wassignificantly lower than that in specimens infected byHPV6/11.However, expression of p21^(WAF1) between the twogroups was not significantly different. Expression of p53 incondyloma acuminatum is likely related to HPV genotype,exoression of p21^(WAF1) was not related to HPV genotype.