Chemokine receptors CXCR4 and CCR5 are indispensable co-receptors for HIV-1 entry into host cells. In our previous study, we identified that dopamine receptor-interacting protein 78（DRi P78） and Na＋-H＋ exchanger r...Chemokine receptors CXCR4 and CCR5 are indispensable co-receptors for HIV-1 entry into host cells. In our previous study, we identified that dopamine receptor-interacting protein 78（DRi P78） and Na＋-H＋ exchanger regulatory factor 1（NHERF1） are the CXCR4 and CCR5 homo- or hetero-dimerinteracting proteins. DRi P78 and NHERF1 are able to influence the co-receptor internalization and intracellular trafficking. Over-expression of NHERF1 affects the ligands or HIV-1 gp120-induced CCR5 internalization and HIV-1 production. It is reasonable to speculate that DRi P78 and NHERF1, as well as the signaling pathways involved in viral replication, would probably affect HIV-1 replication through regulating the co-receptors. In this present study, we designed two short hairpin RNAs（sh RNAs） targeting the DRi P78 and NHERF1, respectively, and constructed the p Lenti6/BLOCK-i T-DEST lentiviral plasmids expressing DRi P78 or NHERF1 sh RNA. The packaged lentiviruses were used to transduce the widely-applied HIV-1 model cell line GHOST（3）. Then, cells with stable knockdown were established through selecting transduced cells with Blasticidin. This study, for the first time, reported the establishment of the GHOST（3） with DRi P78 and NHERF1 knockdown, which is the first stable cell line with HIV-1 co-receptor-interacting molecular defects.展开更多
讨论电表信号隔离传输的意义,分析目前各种实现手段的优缺点,提出一种数据隔离设计方案.方案采用ELAN公司的EM78P372N单片机为核心,用12 bit AD采样电表输入信号,数字信号通过光电耦合器进行自定义协议与后级单片机通信,实现有效隔离....讨论电表信号隔离传输的意义,分析目前各种实现手段的优缺点,提出一种数据隔离设计方案.方案采用ELAN公司的EM78P372N单片机为核心,用12 bit AD采样电表输入信号,数字信号通过光电耦合器进行自定义协议与后级单片机通信,实现有效隔离.测试结果表明,方案的电路设计合理,准确率高,成本低,安全性能好,具有很好的应用前景.展开更多
基金supported by the Science and Technology Planning Project of Guangdong Province of China（2012B031800267）the Natural Science Foundation of Guangdong Province of China（S2013010011860）the National Natural Science Foundation of China（31200130 and 81371812）
文摘Chemokine receptors CXCR4 and CCR5 are indispensable co-receptors for HIV-1 entry into host cells. In our previous study, we identified that dopamine receptor-interacting protein 78（DRi P78） and Na＋-H＋ exchanger regulatory factor 1（NHERF1） are the CXCR4 and CCR5 homo- or hetero-dimerinteracting proteins. DRi P78 and NHERF1 are able to influence the co-receptor internalization and intracellular trafficking. Over-expression of NHERF1 affects the ligands or HIV-1 gp120-induced CCR5 internalization and HIV-1 production. It is reasonable to speculate that DRi P78 and NHERF1, as well as the signaling pathways involved in viral replication, would probably affect HIV-1 replication through regulating the co-receptors. In this present study, we designed two short hairpin RNAs（sh RNAs） targeting the DRi P78 and NHERF1, respectively, and constructed the p Lenti6/BLOCK-i T-DEST lentiviral plasmids expressing DRi P78 or NHERF1 sh RNA. The packaged lentiviruses were used to transduce the widely-applied HIV-1 model cell line GHOST（3）. Then, cells with stable knockdown were established through selecting transduced cells with Blasticidin. This study, for the first time, reported the establishment of the GHOST（3） with DRi P78 and NHERF1 knockdown, which is the first stable cell line with HIV-1 co-receptor-interacting molecular defects.
文摘讨论电表信号隔离传输的意义,分析目前各种实现手段的优缺点,提出一种数据隔离设计方案.方案采用ELAN公司的EM78P372N单片机为核心,用12 bit AD采样电表输入信号,数字信号通过光电耦合器进行自定义协议与后级单片机通信,实现有效隔离.测试结果表明,方案的电路设计合理,准确率高,成本低,安全性能好,具有很好的应用前景.
