Objective:To explore the neuroprotective effect of pAkt and HIF-1α011 ischemia rats.Methods:The rat model of cerebral ischemia which induced by permanent middle cerebral artery occlusion was established.Silybin were ...Objective:To explore the neuroprotective effect of pAkt and HIF-1α011 ischemia rats.Methods:The rat model of cerebral ischemia which induced by permanent middle cerebral artery occlusion was established.Silybin were given respectively.The behavior was measured by modified Longa method,brain water content were measured by the dry-wet melhod.Infarct volume was measured by image analysis method.Akt.HIF-1 a.Bcl-2,Bax.NF-κB protein expressions were delected by Western blotting.The Akt,HIF-1 a,Bcl-2,Bax,NF-κB mRNA expression were detected by RT-PCR.Results:The control group,low-dose silibinin group and high-dose silibinin group showed paralytic of the left body of rats in various degrees,the brain water content increased and different infarction size.There was no abnormal of the neurobehavioral assessment and no cerebral infarction in the blank group.Compared with the control group,there was no significant improvement of neurological function(t=1.341,P=0.188)or significant changes of the infarct volume(t=1.737.P=0.091)in the low-dose silibinin group,while there was significantly improvement of the neurological function in the high dose silibinin group(t=12.979,P<0.001),and the infarct volume was significantly reduced(/-23.503.P<0.001),the difference had statistically significant.The brain water content of lesion side of the control group increased(t=43.536,P<0.001),while the brain water content of lesion side of the lowdose silibinin group and the high-dose silybin group were significantly reduced(t=25.571,P<0.00l;t=42.426,P<0.001).The differences were statistical significance.The p-Akt 473,p-Akt 308,HIF-1α,Bax,NF-κB protein and the Akt,Bax.NF-κB mRNA expression were increased of the control group,while the Bcl-2 protein and mRNA expression were decreased,the differences were statistically significant(P<0.05).there was no significant change of the Akt protein expression and HIF-1 a mRNA in the control group(P>0.05).In the high dose silybin group,the p-Akt 473,p-Akt 308,HIF-1 a,Bcl-2 protein and Akt.Bcl-2 mRNA expression were increased,while the Bax,NF-κB protein and Bax.NF-K B mRNA expression were decreased,the differences were statistically significant(P<0.05),there was no significant change of the Akt protein expression and HIF-1 a mRNA in the high dose silybin group(P>0.05).Conclusions:pAkt,HIF-1 a have neuroprotective effect on ischemia rats.展开更多
目的:探讨pAKT和NF-κB在卵巢癌组织中的表达及与病理学特征之间的关系。方法:选择2016年4月—2018年3月期间我院收治的卵巢上皮良性肿瘤患者50例(良性组)和卵巢上皮性癌患者70例(卵巢癌组)为观察对象,再选择同期来我院体检的健康者48...目的:探讨pAKT和NF-κB在卵巢癌组织中的表达及与病理学特征之间的关系。方法:选择2016年4月—2018年3月期间我院收治的卵巢上皮良性肿瘤患者50例(良性组)和卵巢上皮性癌患者70例(卵巢癌组)为观察对象,再选择同期来我院体检的健康者48例为对照组,运用免疫组化染色对两组标本中的pAKT和NF-κB蛋白表达程度进行检测,并且对与病理学特征之间的关系进行探讨。结果:卵巢癌组的NF-κB和pAKT蛋白阳性表达率均高于良性组和对照组( P <0.05),但是对照组和良性组比较差异无统计学意义( P >0.05);同时,与未发生淋巴结转移、中+高分化、Ⅰ期+Ⅱ期的卵巢癌组织相比,发生淋巴结转移、低分化以及Ⅲ期+Ⅳ期卵巢癌组织的pAKT和NF-κB阳性率均较高( P <0.05)。结论: pAKT和NF-κB蛋白在卵巢癌组织中的阳性表达率较高,并且与淋巴结转移、组织学分化程度以及临床分期密切相关。展开更多
基金supported by Shandong Province Science and Technology Department research projects,fund project number F003488_1
文摘Objective:To explore the neuroprotective effect of pAkt and HIF-1α011 ischemia rats.Methods:The rat model of cerebral ischemia which induced by permanent middle cerebral artery occlusion was established.Silybin were given respectively.The behavior was measured by modified Longa method,brain water content were measured by the dry-wet melhod.Infarct volume was measured by image analysis method.Akt.HIF-1 a.Bcl-2,Bax.NF-κB protein expressions were delected by Western blotting.The Akt,HIF-1 a,Bcl-2,Bax,NF-κB mRNA expression were detected by RT-PCR.Results:The control group,low-dose silibinin group and high-dose silibinin group showed paralytic of the left body of rats in various degrees,the brain water content increased and different infarction size.There was no abnormal of the neurobehavioral assessment and no cerebral infarction in the blank group.Compared with the control group,there was no significant improvement of neurological function(t=1.341,P=0.188)or significant changes of the infarct volume(t=1.737.P=0.091)in the low-dose silibinin group,while there was significantly improvement of the neurological function in the high dose silibinin group(t=12.979,P<0.001),and the infarct volume was significantly reduced(/-23.503.P<0.001),the difference had statistically significant.The brain water content of lesion side of the control group increased(t=43.536,P<0.001),while the brain water content of lesion side of the lowdose silibinin group and the high-dose silybin group were significantly reduced(t=25.571,P<0.00l;t=42.426,P<0.001).The differences were statistical significance.The p-Akt 473,p-Akt 308,HIF-1α,Bax,NF-κB protein and the Akt,Bax.NF-κB mRNA expression were increased of the control group,while the Bcl-2 protein and mRNA expression were decreased,the differences were statistically significant(P<0.05).there was no significant change of the Akt protein expression and HIF-1 a mRNA in the control group(P>0.05).In the high dose silybin group,the p-Akt 473,p-Akt 308,HIF-1 a,Bcl-2 protein and Akt.Bcl-2 mRNA expression were increased,while the Bax,NF-κB protein and Bax.NF-K B mRNA expression were decreased,the differences were statistically significant(P<0.05),there was no significant change of the Akt protein expression and HIF-1 a mRNA in the high dose silybin group(P>0.05).Conclusions:pAkt,HIF-1 a have neuroprotective effect on ischemia rats.
文摘目的:探讨pAKT和NF-κB在卵巢癌组织中的表达及与病理学特征之间的关系。方法:选择2016年4月—2018年3月期间我院收治的卵巢上皮良性肿瘤患者50例(良性组)和卵巢上皮性癌患者70例(卵巢癌组)为观察对象,再选择同期来我院体检的健康者48例为对照组,运用免疫组化染色对两组标本中的pAKT和NF-κB蛋白表达程度进行检测,并且对与病理学特征之间的关系进行探讨。结果:卵巢癌组的NF-κB和pAKT蛋白阳性表达率均高于良性组和对照组( P <0.05),但是对照组和良性组比较差异无统计学意义( P >0.05);同时,与未发生淋巴结转移、中+高分化、Ⅰ期+Ⅱ期的卵巢癌组织相比,发生淋巴结转移、低分化以及Ⅲ期+Ⅳ期卵巢癌组织的pAKT和NF-κB阳性率均较高( P <0.05)。结论: pAKT和NF-κB蛋白在卵巢癌组织中的阳性表达率较高,并且与淋巴结转移、组织学分化程度以及临床分期密切相关。
