A novel multilayer film based on Au nanoparticles(AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes-chitosan nanocomposite(PANI/MWCNTs/CS) was exploited to fabricate a highly sensitive immunosensor for de...A novel multilayer film based on Au nanoparticles(AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes-chitosan nanocomposite(PANI/MWCNTs/CS) was exploited to fabricate a highly sensitive immunosensor for detecting chlorpyrifos. PANI-coated MWCNTs were prepared by in situ chemical polymerization and carboxylated MWCNTs played an important role in obtaining the thin and uniform coating of PANI resulting in the improved immunosensor response. Au NPs were used as a linker to immobilize chlorpyrifos antibody. The performance of the immunosensor was characterized by means of cyclic voltammetry(CV), electrochemical impedance spectroscopy(EIS) and scanning electron microscopy(SEM), respectively. All variables involved in the preparation process and analytical performance of the immunosensor were optimized.Under optimal conditions(antibody concentration: 5 μg/mL, working buffer pH: 6.5, incubation time: 40 min,incubation temperature: 25℃), the immunosensor exhibited a wide linear range from 0.1 to 40× 10^(-6)mg/mL and from 40 × 10^(-6)mg/mL to 500 × 10^(-6)mg/mL, and with a detection limit of 0.06 × 10^(-6)mg/mL, which provided a valuable tool for the chlorpyrifos detection in real samples.展开更多
基金supported by the National Natural Science Foundation of China (No. 30972055, 31101286)Agricultural Science and Technology Achievements Transformation Fund Projects of the Ministry of Science and Technology of China (No. 2011GB2C60020)Shandong Provincial Natural Science Foundation, China (No. Q2008D03)
文摘A novel multilayer film based on Au nanoparticles(AuNPs) and polyaniline/carboxylated multiwall carbon nanotubes-chitosan nanocomposite(PANI/MWCNTs/CS) was exploited to fabricate a highly sensitive immunosensor for detecting chlorpyrifos. PANI-coated MWCNTs were prepared by in situ chemical polymerization and carboxylated MWCNTs played an important role in obtaining the thin and uniform coating of PANI resulting in the improved immunosensor response. Au NPs were used as a linker to immobilize chlorpyrifos antibody. The performance of the immunosensor was characterized by means of cyclic voltammetry(CV), electrochemical impedance spectroscopy(EIS) and scanning electron microscopy(SEM), respectively. All variables involved in the preparation process and analytical performance of the immunosensor were optimized.Under optimal conditions(antibody concentration: 5 μg/mL, working buffer pH: 6.5, incubation time: 40 min,incubation temperature: 25℃), the immunosensor exhibited a wide linear range from 0.1 to 40× 10^(-6)mg/mL and from 40 × 10^(-6)mg/mL to 500 × 10^(-6)mg/mL, and with a detection limit of 0.06 × 10^(-6)mg/mL, which provided a valuable tool for the chlorpyrifos detection in real samples.
