Lower temperature or polyphosphate limitation is the favorable condition to enrich polyphosphate-accumulating organisms (PAOs) or glycogen accumulating organisms (GAOs) in biological phosphorus removal process. In thi...Lower temperature or polyphosphate limitation is the favorable condition to enrich polyphosphate-accumulating organisms (PAOs) or glycogen accumulating organisms (GAOs) in biological phosphorus removal process. In this study,a sludge highly enriched in PAOs was used to investigate the metabolic pathways and carbon transformation in batch tests under the two conditions for elucidating the contradiction. The results showed that the PAOs are able to take up acetate and store it as polyhydroxyalkanoates (PHA) in anaerobic phase although the poly-P pool is emptied through washing with negligible P release. The stoichiometric ratios of the PHB and PHV production and glycogen degradation to the acetate uptake combined with the Nile blue staining indicated that the PAOs possess the anaerobic metabolism of GAOs using glycogen as the sole energy source,but no transformation of PAOs to GAOs is observed. After reconverting to the normal operation,phosphorus uptake and release are recovered the same as the parent reactor.展开更多
[Objectives]To study the phosphorus removal performance of phosphate accumulating organisms(PAOs).[Methods]Activated sludge from domestic sewage treatment plant was used as the strain source,and phosphate accumulating...[Objectives]To study the phosphorus removal performance of phosphate accumulating organisms(PAOs).[Methods]Activated sludge from domestic sewage treatment plant was used as the strain source,and phosphate accumulating organisms were screened by plate streaking method and dilution coating plate method.Six kinds of excellent phosphate accumulating organisms were obtained by metachromatic granule staining experiment,total phosphorus experiment and simulated sewage phosphorus removal experiment to assist the observation of bac-terial morphology and experiment of phosphorus removal capacity.In addition,the influencing factors of phosphorus removal capacity(nitrogen source,trace metal ions)were analyzed.[Results]In the case of simulated sewage,the phosphorus removal rate of strain b was the highest,reaching 66.25%,while the phosphorus removal rate of strain e and f was about 10%lower than that of the phosphorus uptake experiment.[Conclusions]This study is expected to provide a theoretical reference for the gradual optimization of the screening method of phosphorus re-moval bacteria in domestic sewage treatment.展开更多
基金Sponsored by the Major Science and Technology Program for Water Pollution Control and Treatment (Grant No.2008ZX07207-005-02)
文摘Lower temperature or polyphosphate limitation is the favorable condition to enrich polyphosphate-accumulating organisms (PAOs) or glycogen accumulating organisms (GAOs) in biological phosphorus removal process. In this study,a sludge highly enriched in PAOs was used to investigate the metabolic pathways and carbon transformation in batch tests under the two conditions for elucidating the contradiction. The results showed that the PAOs are able to take up acetate and store it as polyhydroxyalkanoates (PHA) in anaerobic phase although the poly-P pool is emptied through washing with negligible P release. The stoichiometric ratios of the PHB and PHV production and glycogen degradation to the acetate uptake combined with the Nile blue staining indicated that the PAOs possess the anaerobic metabolism of GAOs using glycogen as the sole energy source,but no transformation of PAOs to GAOs is observed. After reconverting to the normal operation,phosphorus uptake and release are recovered the same as the parent reactor.
基金Supported by Zhaoqing University Innovation and Entrepreneurship Training Program for College Students(202310580018).
文摘[Objectives]To study the phosphorus removal performance of phosphate accumulating organisms(PAOs).[Methods]Activated sludge from domestic sewage treatment plant was used as the strain source,and phosphate accumulating organisms were screened by plate streaking method and dilution coating plate method.Six kinds of excellent phosphate accumulating organisms were obtained by metachromatic granule staining experiment,total phosphorus experiment and simulated sewage phosphorus removal experiment to assist the observation of bac-terial morphology and experiment of phosphorus removal capacity.In addition,the influencing factors of phosphorus removal capacity(nitrogen source,trace metal ions)were analyzed.[Results]In the case of simulated sewage,the phosphorus removal rate of strain b was the highest,reaching 66.25%,while the phosphorus removal rate of strain e and f was about 10%lower than that of the phosphorus uptake experiment.[Conclusions]This study is expected to provide a theoretical reference for the gradual optimization of the screening method of phosphorus re-moval bacteria in domestic sewage treatment.
