The paper proposes a new tool to measure the risk in financial market:PaV,which means the hap pening probability with the given loss magnitude,and utilizes Copula function to obtain its computing algorithm.Two cases a...The paper proposes a new tool to measure the risk in financial market:PaV,which means the hap pening probability with the given loss magnitude,and utilizes Copula function to obtain its computing algorithm.Two cases are illustrated for the promising applications of PaV.展开更多
最近阐明了水华蓝藻噬藻体PaV-LD(Planktothrix agardhii Virus isolated from Lake Donghu)的全基因组序列,这是一个含有142个ORF的双链DNA噬藻体。在此,我们对其主要衣壳蛋白基因073R,内肽酶和穿孔素基因123L-124L(PaV-LD基因组中两...最近阐明了水华蓝藻噬藻体PaV-LD(Planktothrix agardhii Virus isolated from Lake Donghu)的全基因组序列,这是一个含有142个ORF的双链DNA噬藻体。在此,我们对其主要衣壳蛋白基因073R,内肽酶和穿孔素基因123L-124L(PaV-LD基因组中两个相邻的ORF)进行了基因克隆与表达分析。将073R克隆后构建原核表达质粒pET-32a-073R,并用IPTG进行诱导表达,073R融合蛋白经纯化后,进行免疫小鼠制备抗体;通过Western blot检测经噬藻体感染宿主细胞后073R的表达时序,结果显示在宿主细胞裂解之初,即PaV-LD感染48h以后073R开始表达,表明073R是一个晚期基因;同时073R推导的氨基酸序列与34株噬藻(菌)体及2株藻病毒(感染真核藻的病毒)的主要衣壳蛋白的氨基酸序列进行序列比对,显示073R与无尾的藻病毒衣壳蛋白亲缘关系更近。PCR扩增内肽酶和穿孔素基因123L-124L,并构建质粒pOP123L-124L,将其转入模式藻集胞藻PCC6803细胞中,质粒pOP123L-124L与藻集胞藻PCC6803基因组发生重组,形成重组藻;测定了重组藻与野生藻的生长速率,并绘制生长曲线;制备超薄切片,进一步比较和观察重组藻与野生藻的超微结构的变化。结果显示重组藻与野生藻存在生长速率与超微形态的显著差异。展开更多
For the most coastal areas where the air is very humid,study on the effect of humidity on the performance of asphalt has its practical significance.However,limited research has been done to investigate the effect of h...For the most coastal areas where the air is very humid,study on the effect of humidity on the performance of asphalt has its practical significance.However,limited research has been done to investigate the effect of humidity on rheological properties of asphalt.In this study,asphalt binders were aged in the pressure aging vessel(PAV)under different conditions of moisture and aging time.Then Dynamic Shear Rheometer(DSR)and Bending Beam Rheometer(BBR)measurements were conducted to obtain the parameters relating to the complex modulus,phase angle,failure temperature,and creep stiffness.It was found that the low temperature performance decreased as evidenced by the results of the creep stiffness.While there were no significant differences among conditioned binders based on complex modulus G*and phase angleδ,it was found that theδvalue increased when the humidity increased from 0% to 80% ,and then theδvalue decreased when the humidity increased to 100% .In addition,the results of the failure temperature indicated that the humidity of 80% would be a critical point for the high temperature performance of the asphalt binders.展开更多
A new ephemeris,period change analysis and light curve modeling of the W UMa-type eclipsing binary BF Pav are presented in this study.Light curves of the system taken in BVRI filters from two observatories,in Australi...A new ephemeris,period change analysis and light curve modeling of the W UMa-type eclipsing binary BF Pav are presented in this study.Light curves of the system taken in BVRI filters from two observatories,in Australia and Argentina,were modeled using the Wilson-Devinney code.The results of this analysis demonstrate that BF Pav is a contact binary system with a photometric mass ratio q=1.460 ± 0.014,a fillout factor f=12.5%,an inclination of 87.97 ± 0.45 deg and a cold spot on the secondary component.By applying the distance modulus formula,the distance of BF Pav was calculated to be d=268 ± 18 pc which is in good agreement with the Gaia EDR3 distance.We obtain an orbital period increase at a rate of 0.142 s century-1 due to a quadratic trend in the O-C diagram.Also,an alternative sudden period jump probably occurred which could be interpreted as a rapid mass transfer from the lower mass star to its companion of about ΔM=2.45 × 10-6 M⊙.Furthermore,there is an oscillatory behavior with a period of 18.3±0.3 yr.Since BF Pav does not seem to have significant magnetic activity,this behavior could be interpreted as the light-time effect caused by an undetected third body in this system.In this case,the probability for the third body to be a low mass star with M≥ 0.075 M⊙or a brown dwarf is 5.4% and 94.6% respectively.If we assume i’=90°,a3=8.04±0.33 AU.The mass of the secondary component was also determined following two different methods which result close to each other.展开更多
Panulirus argus virus 1 (PaV1) is considered a major threat to spiny lobsters Panulirus argus. In this study Geospatial analysis was used to analyze PaV1 distribution in an artisanal fishery of spiny lobster Panulirus...Panulirus argus virus 1 (PaV1) is considered a major threat to spiny lobsters Panulirus argus. In this study Geospatial analysis was used to analyze PaV1 distribution in an artisanal fishery of spiny lobster Panulirus argus population from the north coast of the Yucatan Peninsula. Adult and sub-adult P. argus and seabed coverage data were collected from thirty artisanal fishing sites. Five seabed coverage types were identified: seagrass;sand/seagrass mixture;sand only;coral/sand mixture;and seaweed. No juveniles were examined. Of the 358 collected lobsters, PaV1 was identified in four organisms (three sub-adults and one adult) from two fishing sites (termed A & B), both found in a seagrass coverage area. Overall prevalence was of 1.12%. Prevalence was of 20% (2/10) at one site and of 12.6% (2/16) at the other.展开更多
文摘The paper proposes a new tool to measure the risk in financial market:PaV,which means the hap pening probability with the given loss magnitude,and utilizes Copula function to obtain its computing algorithm.Two cases are illustrated for the promising applications of PaV.
文摘最近阐明了水华蓝藻噬藻体PaV-LD(Planktothrix agardhii Virus isolated from Lake Donghu)的全基因组序列,这是一个含有142个ORF的双链DNA噬藻体。在此,我们对其主要衣壳蛋白基因073R,内肽酶和穿孔素基因123L-124L(PaV-LD基因组中两个相邻的ORF)进行了基因克隆与表达分析。将073R克隆后构建原核表达质粒pET-32a-073R,并用IPTG进行诱导表达,073R融合蛋白经纯化后,进行免疫小鼠制备抗体;通过Western blot检测经噬藻体感染宿主细胞后073R的表达时序,结果显示在宿主细胞裂解之初,即PaV-LD感染48h以后073R开始表达,表明073R是一个晚期基因;同时073R推导的氨基酸序列与34株噬藻(菌)体及2株藻病毒(感染真核藻的病毒)的主要衣壳蛋白的氨基酸序列进行序列比对,显示073R与无尾的藻病毒衣壳蛋白亲缘关系更近。PCR扩增内肽酶和穿孔素基因123L-124L,并构建质粒pOP123L-124L,将其转入模式藻集胞藻PCC6803细胞中,质粒pOP123L-124L与藻集胞藻PCC6803基因组发生重组,形成重组藻;测定了重组藻与野生藻的生长速率,并绘制生长曲线;制备超薄切片,进一步比较和观察重组藻与野生藻的超微结构的变化。结果显示重组藻与野生藻存在生长速率与超微形态的显著差异。
基金supported by the National Natural Science Fund Project of China(NSFC)(No.:51278173)the Natural Science Fund Project of Jiangsu Provincial Communications Department(No.:SBK201120606)
文摘For the most coastal areas where the air is very humid,study on the effect of humidity on the performance of asphalt has its practical significance.However,limited research has been done to investigate the effect of humidity on rheological properties of asphalt.In this study,asphalt binders were aged in the pressure aging vessel(PAV)under different conditions of moisture and aging time.Then Dynamic Shear Rheometer(DSR)and Bending Beam Rheometer(BBR)measurements were conducted to obtain the parameters relating to the complex modulus,phase angle,failure temperature,and creep stiffness.It was found that the low temperature performance decreased as evidenced by the results of the creep stiffness.While there were no significant differences among conditioned binders based on complex modulus G*and phase angleδ,it was found that theδvalue increased when the humidity increased from 0% to 80% ,and then theδvalue decreased when the humidity increased to 100% .In addition,the results of the failure temperature indicated that the humidity of 80% would be a critical point for the high temperature performance of the asphalt binders.
基金based on the Binary Systems of South and North Project (http:// bsnp.info)。
文摘A new ephemeris,period change analysis and light curve modeling of the W UMa-type eclipsing binary BF Pav are presented in this study.Light curves of the system taken in BVRI filters from two observatories,in Australia and Argentina,were modeled using the Wilson-Devinney code.The results of this analysis demonstrate that BF Pav is a contact binary system with a photometric mass ratio q=1.460 ± 0.014,a fillout factor f=12.5%,an inclination of 87.97 ± 0.45 deg and a cold spot on the secondary component.By applying the distance modulus formula,the distance of BF Pav was calculated to be d=268 ± 18 pc which is in good agreement with the Gaia EDR3 distance.We obtain an orbital period increase at a rate of 0.142 s century-1 due to a quadratic trend in the O-C diagram.Also,an alternative sudden period jump probably occurred which could be interpreted as a rapid mass transfer from the lower mass star to its companion of about ΔM=2.45 × 10-6 M⊙.Furthermore,there is an oscillatory behavior with a period of 18.3±0.3 yr.Since BF Pav does not seem to have significant magnetic activity,this behavior could be interpreted as the light-time effect caused by an undetected third body in this system.In this case,the probability for the third body to be a low mass star with M≥ 0.075 M⊙or a brown dwarf is 5.4% and 94.6% respectively.If we assume i’=90°,a3=8.04±0.33 AU.The mass of the secondary component was also determined following two different methods which result close to each other.
文摘Panulirus argus virus 1 (PaV1) is considered a major threat to spiny lobsters Panulirus argus. In this study Geospatial analysis was used to analyze PaV1 distribution in an artisanal fishery of spiny lobster Panulirus argus population from the north coast of the Yucatan Peninsula. Adult and sub-adult P. argus and seabed coverage data were collected from thirty artisanal fishing sites. Five seabed coverage types were identified: seagrass;sand/seagrass mixture;sand only;coral/sand mixture;and seaweed. No juveniles were examined. Of the 358 collected lobsters, PaV1 was identified in four organisms (three sub-adults and one adult) from two fishing sites (termed A & B), both found in a seagrass coverage area. Overall prevalence was of 1.12%. Prevalence was of 20% (2/10) at one site and of 12.6% (2/16) at the other.
