Recently, a new radio millisecond pulsar(MSP) J1740-5340B, hosted in the globular cluster(GC) NGC 6397,was reported with a 5.78 ms spin period in an eclipsing binary system with a 1.97 days orbital period. Based on a ...Recently, a new radio millisecond pulsar(MSP) J1740-5340B, hosted in the globular cluster(GC) NGC 6397,was reported with a 5.78 ms spin period in an eclipsing binary system with a 1.97 days orbital period. Based on a modified radio ephemeris updated by tool tempo2, we analyze the ~15 yr γ-ray data obtained from the Large Area Telescope on board the Fermi Gamma-ray Space Telescope and detect PSR J1740-5340B's γ-ray pulsation at a confidence level of ~4σ with a weighted H-test value of ~26. By performing a phase-resolved analysis, the γ-ray luminosity in on-pulse interval of PSR J1740-5340B is L_(γ)~ 3.8 × 10^(33) erg s^(-1) using NGC 6397's distance of 2.48 kpc. And γ-rays from the on-pulse part of PSR J1740-5340B contribute ~90% of the total observed γ-ray emissions from NGC 6397. No significant γ-ray pulsation of another MSP J1740-5340A in the GC is detected.Considering that the previous four cases of MSPs in GCs, more data in γ-ray, X-ray, and radio are encouraged to finally confirm the γ-ray emissions from MSP J1740-5340B, especially starving for a precise ephemeris.展开更多
BACKGROUND Advanced glycation end products(AGEs)are diabetic metabolic toxic products that cannot be ignored.Nε-(carboxymethyl)lysine(CML),a component of AGEs,could increase macrophage lipid uptake,promote foam cell ...BACKGROUND Advanced glycation end products(AGEs)are diabetic metabolic toxic products that cannot be ignored.Nε-(carboxymethyl)lysine(CML),a component of AGEs,could increase macrophage lipid uptake,promote foam cell formation,and thereby accelerate atherosclerosis.The receptor for AGEs(RAGE)and cluster of differentiation 36(CD36)were the receptors of CML.However,it is still unknown whether RAGE and CD36 play key roles in CML-promoted lipid uptake.AIM Our study aimed to explore the role of RAGE and CD36 in CML-induced macrophage lipid uptake.METHODS In this study,we examined the effect of CML on lipid uptake by Raw264.7 macrophages.After adding 10 mmol/L CML,the lipid accumulation in macrophages was confirmed by oil red O staining.Expression changes of CD36 and RAGE were detected with immunoblotting and quantitative real-time polymerase chain reaction.The interaction between CML with CD36 and RAGE was verified by immunoprecipitation.We synthesized a novel N-succinimidyl-4-18Ffluorobenzoate-CML radioactive probe.Radioactive receptor-ligand binding assays were performed to test the binding affinity between CML with CD36 and RAGE.The effects of blocking CD36 or RAGE on CML-promoting lipid uptake were also detected.RESULTS The study revealed that CML significantly promoted lipid uptake by macrophages.Immunoprecipitation and radioactive receptor-ligand binding assays indicated that CML could specifically bind to both CD36 and RAGE.CML had a higher affinity for CD36 than RAGE.ARG82,ASN71,and THR70 were the potential interacting amino acids that CD36 binds to CML Anti-CD36 and anti-RAGE could block the uptake of CML by macrophages.The lipid uptake promotion effect of CML was significantly attenuated after blocking CD36 or RAGE.CONCLUSION Our results suggest that the binding of CML with CD36 and RAGE promotes macrophage lipid uptake.展开更多
A hierarchical cluster analysis has been made on the geomagnetic and geoelectric data of Nagoya ( Φ =21.1°), Japan (1978 1981, 1985, 1987) and geomagnetic data of Wuchang ( Φ =19.1°), China (1985 199...A hierarchical cluster analysis has been made on the geomagnetic and geoelectric data of Nagoya ( Φ =21.1°), Japan (1978 1981, 1985, 1987) and geomagnetic data of Wuchang ( Φ =19.1°), China (1985 1995). From the cluster diagram it is seen that the monthly mean occurrence of Pc3 4 observed at these two sites can be best grouped into 3 clusters.展开更多
Open clusters are the basic building blocks that serve as a laboratory for the study of young stellar populations in the Milky Way.Variable stars in open clusters provide a unique way to accurately probe the internal ...Open clusters are the basic building blocks that serve as a laboratory for the study of young stellar populations in the Milky Way.Variable stars in open clusters provide a unique way to accurately probe the internal structure,temporal and dynamical evolutionary stages of individual stars and the host cluster.The most powerful tool for such studies is time-domain photometric observations.This paper follows the route of our previous work,concentrating on a photometric search for variable stars in NGC 884.The target cluster is the companion of NGC869,forming the well-known double cluster system that is gravitationally bound.From the observation run in 2016 November,a total of 9247 B-band CCD images and 8218Ⅴ-band CCD images were obtained.We detected a total of 15 stars with variability in visual brightness,including five Be stars,three eclipsing binaries,and seven of unknown types.Two new variable stars were discovered in this work.We also compared the variable star content of NGC 884 with its companion NGC 869.展开更多
基金supported in part by the National Natural Science Foundation of China Nos. 12163006 and 12233006the Basic Research Program of Yunnan Province No. 202201AT070137+1 种基金the joint foundation of Department of Science and Technology of Yunnan Province and Yunnan University No. 202201BF070001-020support by the Xingdian Talent Support Plan-Youth Project。
文摘Recently, a new radio millisecond pulsar(MSP) J1740-5340B, hosted in the globular cluster(GC) NGC 6397,was reported with a 5.78 ms spin period in an eclipsing binary system with a 1.97 days orbital period. Based on a modified radio ephemeris updated by tool tempo2, we analyze the ~15 yr γ-ray data obtained from the Large Area Telescope on board the Fermi Gamma-ray Space Telescope and detect PSR J1740-5340B's γ-ray pulsation at a confidence level of ~4σ with a weighted H-test value of ~26. By performing a phase-resolved analysis, the γ-ray luminosity in on-pulse interval of PSR J1740-5340B is L_(γ)~ 3.8 × 10^(33) erg s^(-1) using NGC 6397's distance of 2.48 kpc. And γ-rays from the on-pulse part of PSR J1740-5340B contribute ~90% of the total observed γ-ray emissions from NGC 6397. No significant γ-ray pulsation of another MSP J1740-5340A in the GC is detected.Considering that the previous four cases of MSPs in GCs, more data in γ-ray, X-ray, and radio are encouraged to finally confirm the γ-ray emissions from MSP J1740-5340B, especially starving for a precise ephemeris.
基金Supported by The National Natural Science Foundation of China,No.82070455Natural Science Foundation of Jiangsu Province,No.BK20201225Medical Innovation Team Project of Jiangsu Province,No.CXTDA2017010。
文摘BACKGROUND Advanced glycation end products(AGEs)are diabetic metabolic toxic products that cannot be ignored.Nε-(carboxymethyl)lysine(CML),a component of AGEs,could increase macrophage lipid uptake,promote foam cell formation,and thereby accelerate atherosclerosis.The receptor for AGEs(RAGE)and cluster of differentiation 36(CD36)were the receptors of CML.However,it is still unknown whether RAGE and CD36 play key roles in CML-promoted lipid uptake.AIM Our study aimed to explore the role of RAGE and CD36 in CML-induced macrophage lipid uptake.METHODS In this study,we examined the effect of CML on lipid uptake by Raw264.7 macrophages.After adding 10 mmol/L CML,the lipid accumulation in macrophages was confirmed by oil red O staining.Expression changes of CD36 and RAGE were detected with immunoblotting and quantitative real-time polymerase chain reaction.The interaction between CML with CD36 and RAGE was verified by immunoprecipitation.We synthesized a novel N-succinimidyl-4-18Ffluorobenzoate-CML radioactive probe.Radioactive receptor-ligand binding assays were performed to test the binding affinity between CML with CD36 and RAGE.The effects of blocking CD36 or RAGE on CML-promoting lipid uptake were also detected.RESULTS The study revealed that CML significantly promoted lipid uptake by macrophages.Immunoprecipitation and radioactive receptor-ligand binding assays indicated that CML could specifically bind to both CD36 and RAGE.CML had a higher affinity for CD36 than RAGE.ARG82,ASN71,and THR70 were the potential interacting amino acids that CD36 binds to CML Anti-CD36 and anti-RAGE could block the uptake of CML by macrophages.The lipid uptake promotion effect of CML was significantly attenuated after blocking CD36 or RAGE.CONCLUSION Our results suggest that the binding of CML with CD36 and RAGE promotes macrophage lipid uptake.
文摘A hierarchical cluster analysis has been made on the geomagnetic and geoelectric data of Nagoya ( Φ =21.1°), Japan (1978 1981, 1985, 1987) and geomagnetic data of Wuchang ( Φ =19.1°), China (1985 1995). From the cluster diagram it is seen that the monthly mean occurrence of Pc3 4 observed at these two sites can be best grouped into 3 clusters.
基金the National Natural Science Foundation of China(NSFC)through grants 12003022,12373035,12233009 and 12173047support from the Youth Innovation Promotion Association of the CAS(grant No.2022055)。
文摘Open clusters are the basic building blocks that serve as a laboratory for the study of young stellar populations in the Milky Way.Variable stars in open clusters provide a unique way to accurately probe the internal structure,temporal and dynamical evolutionary stages of individual stars and the host cluster.The most powerful tool for such studies is time-domain photometric observations.This paper follows the route of our previous work,concentrating on a photometric search for variable stars in NGC 884.The target cluster is the companion of NGC869,forming the well-known double cluster system that is gravitationally bound.From the observation run in 2016 November,a total of 9247 B-band CCD images and 8218Ⅴ-band CCD images were obtained.We detected a total of 15 stars with variability in visual brightness,including five Be stars,three eclipsing binaries,and seven of unknown types.Two new variable stars were discovered in this work.We also compared the variable star content of NGC 884 with its companion NGC 869.
