目的探讨沉默纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)对人肺腺癌PC-9细胞多西他赛敏感性的影响。方法Western blot法检测人正常支气管上皮细胞株BEAS-2B及人肺腺癌细胞株PC-9中的FGL1蛋白表达情况。以siRNA沉默PC-9细胞株中...目的探讨沉默纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)对人肺腺癌PC-9细胞多西他赛敏感性的影响。方法Western blot法检测人正常支气管上皮细胞株BEAS-2B及人肺腺癌细胞株PC-9中的FGL1蛋白表达情况。以siRNA沉默PC-9细胞株中FGL1基因,CCK-8法检测沉默FGL1对PC-9细胞增殖的抑制作用及对多西他赛敏感性的影响。结果与BEAS-2B细胞株比较,FGL1在PC9细胞株中呈高表达,其蛋白相对表达量是BEAS-2B细胞株的6.5倍,差异具有显著性(P<0.01)。通过转染FGL1siRNA沉默FGL1可增强多西他赛对PC-9细胞增殖的抑制作用。与FGL1siNC组细胞相比,FGL1siRNA组PC-9细胞的IC50值明显降低,差异具有显著性(P<0.01)。结论特异性沉默FGL1基因可抑制人肺腺癌细胞PC-9中FGL1的表达,抑制PC-9细胞的增殖,提高对多西他赛的敏感性。展开更多
AIM: To present an inclusion criterion for patients who have suffered bilateral amputation in order to be treated with the supplementary resuscitation treatment which is hereby proposed by the author.METHODS: This wor...AIM: To present an inclusion criterion for patients who have suffered bilateral amputation in order to be treated with the supplementary resuscitation treatment which is hereby proposed by the author.METHODS: This work is based on a Retrospective Cohort model so that a certainly lethal risk to the control group is avoided.RESULTS: This paper presents a hypothesis on acupunctural PC-9 Zhong chong point, further supported by previous statistical work recorded for the K-1 Yong quan resuscitation point.CONCLUSION: Thanks to the application of the resuscitation maneuver herein proposed on the previously mentioned point, patients with bilateral amputation would have another alternative treatment available in case basic and advanced CPR should fail.展开更多
Objective:To investigate the effects of Intron on the EMT capability of non-small cell lung cancer cell line PC-9.Methods:Firstly,using the psiCHECK-2 plasmid as a basic framework to construct the recombinant plasmid ...Objective:To investigate the effects of Intron on the EMT capability of non-small cell lung cancer cell line PC-9.Methods:Firstly,using the psiCHECK-2 plasmid as a basic framework to construct the recombinant plasmid of psiCHECK-2-Intron dual-luciferase reporter gene;secondly,the psiCHECK-2-Intron and psiCHECK-2 were transfected into PC-9 cells respectively.The migration and invasion abilities of PC-9 cells were analyzed by Matrigel assay.The expression changes of EMT related hallmarks,including N-cadherin,β-catenin and snail,were detected by qRT-PCR and Western Blotting.Results:Compared with the control group,the migration and invasion abilities of PC-9 cells in Intron group significantly decreased(p<0.001).The expression of N-cadherin,β-catenin and snail also down-regulated(p<0.001).Conclusion:The introns could inhibit the EMT of PC-9 cells.展开更多
文摘目的探讨沉默纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)对人肺腺癌PC-9细胞多西他赛敏感性的影响。方法Western blot法检测人正常支气管上皮细胞株BEAS-2B及人肺腺癌细胞株PC-9中的FGL1蛋白表达情况。以siRNA沉默PC-9细胞株中FGL1基因,CCK-8法检测沉默FGL1对PC-9细胞增殖的抑制作用及对多西他赛敏感性的影响。结果与BEAS-2B细胞株比较,FGL1在PC9细胞株中呈高表达,其蛋白相对表达量是BEAS-2B细胞株的6.5倍,差异具有显著性(P<0.01)。通过转染FGL1siRNA沉默FGL1可增强多西他赛对PC-9细胞增殖的抑制作用。与FGL1siNC组细胞相比,FGL1siRNA组PC-9细胞的IC50值明显降低,差异具有显著性(P<0.01)。结论特异性沉默FGL1基因可抑制人肺腺癌细胞PC-9中FGL1的表达,抑制PC-9细胞的增殖,提高对多西他赛的敏感性。
文摘AIM: To present an inclusion criterion for patients who have suffered bilateral amputation in order to be treated with the supplementary resuscitation treatment which is hereby proposed by the author.METHODS: This work is based on a Retrospective Cohort model so that a certainly lethal risk to the control group is avoided.RESULTS: This paper presents a hypothesis on acupunctural PC-9 Zhong chong point, further supported by previous statistical work recorded for the K-1 Yong quan resuscitation point.CONCLUSION: Thanks to the application of the resuscitation maneuver herein proposed on the previously mentioned point, patients with bilateral amputation would have another alternative treatment available in case basic and advanced CPR should fail.
基金National Natural Science Foundation of China(No.81660474)。
文摘Objective:To investigate the effects of Intron on the EMT capability of non-small cell lung cancer cell line PC-9.Methods:Firstly,using the psiCHECK-2 plasmid as a basic framework to construct the recombinant plasmid of psiCHECK-2-Intron dual-luciferase reporter gene;secondly,the psiCHECK-2-Intron and psiCHECK-2 were transfected into PC-9 cells respectively.The migration and invasion abilities of PC-9 cells were analyzed by Matrigel assay.The expression changes of EMT related hallmarks,including N-cadherin,β-catenin and snail,were detected by qRT-PCR and Western Blotting.Results:Compared with the control group,the migration and invasion abilities of PC-9 cells in Intron group significantly decreased(p<0.001).The expression of N-cadherin,β-catenin and snail also down-regulated(p<0.001).Conclusion:The introns could inhibit the EMT of PC-9 cells.
