多聚结合蛋白1[poly(rC)-binding protein 1,PCBP1]是RNA结合蛋白家族的成员之一,它广泛表达于各种人体正常组织中,并在多种生物学过程中发挥重要的作用,比如从不同水平调控基因表达、病毒的复制、铁离子转运等。同时,PCBP1作为一种潜...多聚结合蛋白1[poly(rC)-binding protein 1,PCBP1]是RNA结合蛋白家族的成员之一,它广泛表达于各种人体正常组织中,并在多种生物学过程中发挥重要的作用,比如从不同水平调控基因表达、病毒的复制、铁离子转运等。同时,PCBP1作为一种潜在的肿瘤抑制因子,参与多种肿瘤的发生、发展、转移等过程,这为理解肿瘤发生的机制提供了新思路。在本篇综述中,我们简单介绍了PCBP1的结构和功能,并从肿瘤发生发展、侵袭或转移、肿瘤干细胞及肿瘤化疗等方面着重讨论了它在多种肿瘤疾病中可能的作用机制,旨在为临床治疗肿瘤提供一种可靠的靶标基因。展开更多
Introduction:Siva-1,as a pro-apoptotic protein,has been shown to induce extensive apoptosis in a number of different cell lines.In our previous study,we showed that overexpressed Siva-1 decreased the apoptosis of gast...Introduction:Siva-1,as a pro-apoptotic protein,has been shown to induce extensive apoptosis in a number of different cell lines.In our previous study,we showed that overexpressed Siva-1 decreased the apoptosis of gastric cancer cells.So,we believe that it can also work as an anti-apoptotic protein.The present study aimed to determine the specific role of Siva-1 in anticancer drug resistance in gastric cancer in vivo and in vitro and preliminarily reveal the mechanism.Materials and Methods:A vincristine-resistant MKN-28/VCR gastric cancer cell line with stably downregulated Siva-1 was established.The effect of Siva-1 downregulation on chemotherapeutic drug resistance was assessed by measuring the IC50 and pump rate of doxorubicin.Proliferation,apoptosis of cells,and cell cycle were detected via colony formation assay andflow cytometry,respectively.Additionally,migration and invasion of cells was detected via wound healing and transwell assays.Moreover,we determined in vivo effects of LV-Siva-1-RNAi on tumor size,and apoptotic cells in tumor tissues were detected using TUNEL and hematoxylin and eosin staining.Results:Siva-1 downregulation reduced the pump rate of doxorubicin and enhanced the response to drug treatment.Siva-1 negatively regulated proliferation and promoted apoptosis of cells by potentiality G2-M phase arresting.Inhibition of Siva-1 expression in MKN-28/VCR cells significantly weakened wound healing ability and decreased invasion ability.Poly(C)-binding protein 1(PCBP1)was identified as a Siva-1-interacting protein in yeast two-hybrid screening.Semiquantitative RT-PCR and western blotting revealed that Siva-1 downregulation could inhibit expression of PCBP1,Akt,and NF-κB and eventually decrease the expression of MDR1 and MRP1.Conclusion:The current study demonstrated that the downregulation of Siva-1,which functions as a regulator of MDR1 and MRP1 gene expression in gastric cancer cells by inhibiting PCBP1/Akt/NF-κB signaling pathway expression,enhanced the sensitivity of gastric cancer cells to certain chemotherapies.展开更多
Poly(rC)结合蛋白1[poly(rC)-binding protein 1,PCBP1]是一种相对分子质量为38000的RNA或DNA结合蛋白。众所周知,PCBP1通过其特有的KH结构,参与了细胞内的转录和转录后调控,包括选择性的pre-mRNA剪接、mRNA稳定性和翻译。PCBP1不仅在...Poly(rC)结合蛋白1[poly(rC)-binding protein 1,PCBP1]是一种相对分子质量为38000的RNA或DNA结合蛋白。众所周知,PCBP1通过其特有的KH结构,参与了细胞内的转录和转录后调控,包括选择性的pre-mRNA剪接、mRNA稳定性和翻译。PCBP1不仅在促进神经干细胞的增殖和调控轴突导向相关基因的表达中至关重要,而且可以调节疾病相关基因的表达来影响神经退行性疾病的病理生理过程,调控细胞增殖和转移基因影响神经肿瘤细胞的行为,影响炎症细胞的活化和炎症反应的程度,以此参与多种神经疾病的发病机制。综上所述,深入研究PCBP1的功能可以帮助我们更好地理解其分子机制,并为神经系统疾病的治疗提供新的思路。展开更多
文摘多聚结合蛋白1[poly(rC)-binding protein 1,PCBP1]是RNA结合蛋白家族的成员之一,它广泛表达于各种人体正常组织中,并在多种生物学过程中发挥重要的作用,比如从不同水平调控基因表达、病毒的复制、铁离子转运等。同时,PCBP1作为一种潜在的肿瘤抑制因子,参与多种肿瘤的发生、发展、转移等过程,这为理解肿瘤发生的机制提供了新思路。在本篇综述中,我们简单介绍了PCBP1的结构和功能,并从肿瘤发生发展、侵袭或转移、肿瘤干细胞及肿瘤化疗等方面着重讨论了它在多种肿瘤疾病中可能的作用机制,旨在为临床治疗肿瘤提供一种可靠的靶标基因。
基金supported by grants from the Major Project of Science and Technology of Guangxi Zhuang Autonomous Region(Grant No.AA22096018)the Natural Science Foundation of China(Grant Nos.82260468&81660416).
文摘Introduction:Siva-1,as a pro-apoptotic protein,has been shown to induce extensive apoptosis in a number of different cell lines.In our previous study,we showed that overexpressed Siva-1 decreased the apoptosis of gastric cancer cells.So,we believe that it can also work as an anti-apoptotic protein.The present study aimed to determine the specific role of Siva-1 in anticancer drug resistance in gastric cancer in vivo and in vitro and preliminarily reveal the mechanism.Materials and Methods:A vincristine-resistant MKN-28/VCR gastric cancer cell line with stably downregulated Siva-1 was established.The effect of Siva-1 downregulation on chemotherapeutic drug resistance was assessed by measuring the IC50 and pump rate of doxorubicin.Proliferation,apoptosis of cells,and cell cycle were detected via colony formation assay andflow cytometry,respectively.Additionally,migration and invasion of cells was detected via wound healing and transwell assays.Moreover,we determined in vivo effects of LV-Siva-1-RNAi on tumor size,and apoptotic cells in tumor tissues were detected using TUNEL and hematoxylin and eosin staining.Results:Siva-1 downregulation reduced the pump rate of doxorubicin and enhanced the response to drug treatment.Siva-1 negatively regulated proliferation and promoted apoptosis of cells by potentiality G2-M phase arresting.Inhibition of Siva-1 expression in MKN-28/VCR cells significantly weakened wound healing ability and decreased invasion ability.Poly(C)-binding protein 1(PCBP1)was identified as a Siva-1-interacting protein in yeast two-hybrid screening.Semiquantitative RT-PCR and western blotting revealed that Siva-1 downregulation could inhibit expression of PCBP1,Akt,and NF-κB and eventually decrease the expression of MDR1 and MRP1.Conclusion:The current study demonstrated that the downregulation of Siva-1,which functions as a regulator of MDR1 and MRP1 gene expression in gastric cancer cells by inhibiting PCBP1/Akt/NF-κB signaling pathway expression,enhanced the sensitivity of gastric cancer cells to certain chemotherapies.
文摘Poly(rC)结合蛋白1[poly(rC)-binding protein 1,PCBP1]是一种相对分子质量为38000的RNA或DNA结合蛋白。众所周知,PCBP1通过其特有的KH结构,参与了细胞内的转录和转录后调控,包括选择性的pre-mRNA剪接、mRNA稳定性和翻译。PCBP1不仅在促进神经干细胞的增殖和调控轴突导向相关基因的表达中至关重要,而且可以调节疾病相关基因的表达来影响神经退行性疾病的病理生理过程,调控细胞增殖和转移基因影响神经肿瘤细胞的行为,影响炎症细胞的活化和炎症反应的程度,以此参与多种神经疾病的发病机制。综上所述,深入研究PCBP1的功能可以帮助我们更好地理解其分子机制,并为神经系统疾病的治疗提供新的思路。
