目的基于ITS序列用PCR-RFLP方法鉴别北柴胡药材掺伪藏柴胡的方法。方法分析并筛选出藏柴胡特有的限制性内切酶AseⅠ,用Primer Premier 5.0设计特异性引物,对引物PCR扩增条件和酶切试验进行优化,并对该方法的准确性进行了考察。结果PCR...目的基于ITS序列用PCR-RFLP方法鉴别北柴胡药材掺伪藏柴胡的方法。方法分析并筛选出藏柴胡特有的限制性内切酶AseⅠ,用Primer Premier 5.0设计特异性引物,对引物PCR扩增条件和酶切试验进行优化,并对该方法的准确性进行了考察。结果PCR扩增的目的片段为331 bp,且建立的PCR反应方法对不同的酶均具有适应性。限制性内切酶AseⅠ将藏柴胡切成79 bp和252 bp两个片段,而北柴胡及其他柴胡均不能被酶切,且掺伪检出限为1%。结论PCR-RFLP方法实现了准确鉴别北柴胡中的混伪品藏柴胡。展开更多
Using the BrdU antibody technique followed by an immuno-chemical staining(BAT),the amplification o f DNA fragments specific to human Y chromosome on cell specimen slides was efficiently detected. Whether direct BrdU i...Using the BrdU antibody technique followed by an immuno-chemical staining(BAT),the amplification o f DNA fragments specific to human Y chromosome on cell specimen slides was efficiently detected. Whether direct BrdU incorporation into PCR products or in situ hybridization with PCR products on slides, the amplified targetDNA fragments of specimen were visualized by BAT under the microscope. The availability of BAT and differencesin the sensitivity and efficiency between BAT and dig--if-dUTP labeling in cell in situ PCR were disCussed.展开更多
文摘Using the BrdU antibody technique followed by an immuno-chemical staining(BAT),the amplification o f DNA fragments specific to human Y chromosome on cell specimen slides was efficiently detected. Whether direct BrdU incorporation into PCR products or in situ hybridization with PCR products on slides, the amplified targetDNA fragments of specimen were visualized by BAT under the microscope. The availability of BAT and differencesin the sensitivity and efficiency between BAT and dig--if-dUTP labeling in cell in situ PCR were disCussed.
