A method based on degenerate Oligo primed polymerase chain reaction (PCR) and random amplification of cDNA end (RACE) PCR for cloning a full length cDNA is described. An Amorpha fruticosa cDNA clone encoding UDP gluco...A method based on degenerate Oligo primed polymerase chain reaction (PCR) and random amplification of cDNA end (RACE) PCR for cloning a full length cDNA is described. An Amorpha fruticosa cDNA clone encoding UDP glucose pyrophosphorylase (UGP), a key enzyme producing UDP glucose in the synthesis of sucrose and cellulose, is cloned by using this method. We design 5’ RACE primers based on UGPA1 fragment, which obtains from degenerate PCR. Inverse PCR and nested PCR enable cloning of the remainder 5’ and 3’ end fragments of the gene. The deduced amino acid sequence exhibits significant homology with the other UGP genes cloned. This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes.展开更多
介绍了一种接收解码器,它是一种广泛应用于三网融合的信源接收设备,它可以将接收到的MPTS多节目传输流转换为多个SPTS单节目传输流,并且通过TS OVER IP的形式将各个节目分别输出到不同的IP主机或端口。详细介绍了整个系统各个模块的功...介绍了一种接收解码器,它是一种广泛应用于三网融合的信源接收设备,它可以将接收到的MPTS多节目传输流转换为多个SPTS单节目传输流,并且通过TS OVER IP的形式将各个节目分别输出到不同的IP主机或端口。详细介绍了整个系统各个模块的功能和实现过程。展开更多
文摘A method based on degenerate Oligo primed polymerase chain reaction (PCR) and random amplification of cDNA end (RACE) PCR for cloning a full length cDNA is described. An Amorpha fruticosa cDNA clone encoding UDP glucose pyrophosphorylase (UGP), a key enzyme producing UDP glucose in the synthesis of sucrose and cellulose, is cloned by using this method. We design 5’ RACE primers based on UGPA1 fragment, which obtains from degenerate PCR. Inverse PCR and nested PCR enable cloning of the remainder 5’ and 3’ end fragments of the gene. The deduced amino acid sequence exhibits significant homology with the other UGP genes cloned. This method is more simple and inexpensive than screening cDNA library, and can be easily adapted to clone other genes.
