利用SMART(switching mechanismat5’end of RNA transcript)技术,提取果实少量总RNA,经15-25轮LD-PCR扩增获得全长ds-cDNA,构建了海南主栽的食用香蕉巴西蕉(Musa AAA Group Cavendish)果实的cDNA文库。所构建的文库容量为5×106Pfu...利用SMART(switching mechanismat5’end of RNA transcript)技术,提取果实少量总RNA,经15-25轮LD-PCR扩增获得全长ds-cDNA,构建了海南主栽的食用香蕉巴西蕉(Musa AAA Group Cavendish)果实的cDNA文库。所构建的文库容量为5×106Pfuml-1,重组率93%。利用此cDNA文库,采用96孔板PCR法筛选香蕉Actin2基因,测序结果显示,序列全长1723bp,编码区长1134bp,编码378个氨基酸,与蝴蝶兰Actin2基因序列同源率达83%,已递交GenBank,接受号692696。展开更多
To study the distribution characteristics of Hepatitis B virus (HBV) genotypes in groups of the Zhuang nationality of Baishe in Guangxi, the PCR sandwich hybridization-ELISA technique was used to determine the genotyp...To study the distribution characteristics of Hepatitis B virus (HBV) genotypes in groups of the Zhuang nationality of Baishe in Guangxi, the PCR sandwich hybridization-ELISA technique was used to determine the genotypes in 30 patinets of Zhuang nationality with hepatitis B. Geontype B, C, D and non A^F were found in this group, in which 56.6% of them were type D,46.6% type C,33.3% typeA^F, 20% type B, Most patients were found with types C+D, D+B or C+B. It is suggested that there are genotypes D, C, B and non A^F in this area, and the major one was genotype D. There are mixture of genotypes C+D, B+C, D+B in this region, so the HBV genotype might be associated with area and nationality.展开更多
文摘利用SMART(switching mechanismat5’end of RNA transcript)技术,提取果实少量总RNA,经15-25轮LD-PCR扩增获得全长ds-cDNA,构建了海南主栽的食用香蕉巴西蕉(Musa AAA Group Cavendish)果实的cDNA文库。所构建的文库容量为5×106Pfuml-1,重组率93%。利用此cDNA文库,采用96孔板PCR法筛选香蕉Actin2基因,测序结果显示,序列全长1723bp,编码区长1134bp,编码378个氨基酸,与蝴蝶兰Actin2基因序列同源率达83%,已递交GenBank,接受号692696。
文摘To study the distribution characteristics of Hepatitis B virus (HBV) genotypes in groups of the Zhuang nationality of Baishe in Guangxi, the PCR sandwich hybridization-ELISA technique was used to determine the genotypes in 30 patinets of Zhuang nationality with hepatitis B. Geontype B, C, D and non A^F were found in this group, in which 56.6% of them were type D,46.6% type C,33.3% typeA^F, 20% type B, Most patients were found with types C+D, D+B or C+B. It is suggested that there are genotypes D, C, B and non A^F in this area, and the major one was genotype D. There are mixture of genotypes C+D, B+C, D+B in this region, so the HBV genotype might be associated with area and nationality.
文摘7900HT Fast Real-Time PCR可以在35分钟完成96孔板的PCR反应。缩短了加热循环时间，增加了研究人员的工作效率。这套系统包括一个让使用者可以互换的Fast PCR循环模块，一个qMan Fast Universal PCR Master Mix主机和Optical 96-Well Fast Thermal Cycling Hates快速热循环板。
