Taqman PCR-based Methods for Rapid Detection of Salmonella in Pet Food

[ Objective] To establish a Taqman real-time PCR for detection of Salmonella in pet food. [Method] A pair of primers and a probe were designed based on published nucleotide sequence of invA gene encoding the invasion protein of Salmonella enterica. [ Result] The assay detects Salmonella specifically. The detection limit of the real-time PCR was 17 CFU/test （25 uL/test） for the positive strain. This method was effective to detect artificially contaminated pet food. [ Conclusion] The results showed that Taqman PCR assay was rapid and accurate for detection of Salmonella from infected pet food.

急性病毒性心肌炎和心包炎活检组织病因学研究

目的 通过心肌心包活检组织病理学及分子生物学检测对病毒性心肌炎和心包炎进行病因学研究。方法 对所选病例临床特点、活检组织病理以及应用逆转录巢式PCR偶联直接循环核酸测序与序列分析检测病毒结果进行总结。结果 急性心肌炎病理诊断符合率 43 5 %,心肌心包活检组织肠道病毒阳性率 5 1 6 %(16 / 31例 ) ,其中柯萨奇B组 14例 ,E CHO病毒 1例 ,脊髓灰质炎病毒 1例。结论 病毒性心肌炎和心包炎病毒感染以柯萨奇B组病毒为主 ,中重型病例急性炎症期心肌中多有病毒复制 ,病理损伤也较明显。