Plant disease diagnosis in Barbados is based mainly on traditional biochemical and microbiological cultural techniques with infrequent use of molecular diagnostic techniques. The main objective of this study was to pr...Plant disease diagnosis in Barbados is based mainly on traditional biochemical and microbiological cultural techniques with infrequent use of molecular diagnostic techniques. The main objective of this study was to provide a simple, rapid and accurate PCR strategy for identifying plant diseases in Barbados. We used rep-PCR a molecular diagnostic technique which can provide a barcode profile for many bacteria and some pathogenic fungi. Pseudomonas syringe pv. lachrymans was detected in both seed and leaf tissue from an infected field and commercial seed samples of cucumber in Barbados. Pseudomonas syringae pv. lachrymans reference strains were identified by rep-PCR and compared to isolates from field and commercial varieties of cucumber. Leaf and seed isolates had similar molecular profiles to different reference strains of P. syringae pv. lachryrnans and displayed different rep-PCR profiles when compared to each other. Isolates from seed also showed more variation than leaf samples. While this represents the first scientific report of the pathogen on the island, the data suggest that the presence of P. syringae pv. lachrymans in Barbados may be due to both external and internal contamination through the use of imported seed or spread by endemic epiphytes respectively.展开更多
文摘Plant disease diagnosis in Barbados is based mainly on traditional biochemical and microbiological cultural techniques with infrequent use of molecular diagnostic techniques. The main objective of this study was to provide a simple, rapid and accurate PCR strategy for identifying plant diseases in Barbados. We used rep-PCR a molecular diagnostic technique which can provide a barcode profile for many bacteria and some pathogenic fungi. Pseudomonas syringe pv. lachrymans was detected in both seed and leaf tissue from an infected field and commercial seed samples of cucumber in Barbados. Pseudomonas syringae pv. lachrymans reference strains were identified by rep-PCR and compared to isolates from field and commercial varieties of cucumber. Leaf and seed isolates had similar molecular profiles to different reference strains of P. syringae pv. lachryrnans and displayed different rep-PCR profiles when compared to each other. Isolates from seed also showed more variation than leaf samples. While this represents the first scientific report of the pathogen on the island, the data suggest that the presence of P. syringae pv. lachrymans in Barbados may be due to both external and internal contamination through the use of imported seed or spread by endemic epiphytes respectively.
