目的快速制备并分离纯化硫化氢特异性荧光探针(Washington State Probe-5),建立动物组织中硫化氢的荧光探针测定法,并在癌性胸水模型中进行方法适用性验证。方法优化条件分离纯化WSP-5;对荧光探针反应液配制溶剂、DMSO加入体积、pH、反...目的快速制备并分离纯化硫化氢特异性荧光探针(Washington State Probe-5),建立动物组织中硫化氢的荧光探针测定法,并在癌性胸水模型中进行方法适用性验证。方法优化条件分离纯化WSP-5;对荧光探针反应液配制溶剂、DMSO加入体积、pH、反应液溶剂和反应液体积、样品前处理温度、研磨次数、研磨后静置时间等条件进行考察;以S-180腹水瘤细胞建立癌性胸水小鼠模型,测定小鼠各脏器中的硫化氢含量。结果以硅胶和葡聚糖凝胶为固定相,二氯甲烷-甲醇-甲酸(60∶1∶0.1,V/V/V)和二氯甲烷-甲醇(1∶1,V/V)为洗脱剂制备WSP-5纯品;动物组织样本和硫氢化钠标准溶液加入优化后的5倍量冰冷反应液,低温研磨、高速离心,上清液避光孵育12 h,测定荧光强度并计算硫化氢浓度;该方法检测限约0.6μmol·L^(-1),定量限1μmol·L^(-1),在硫氢化钠一定浓度范围内,浓度与荧光强度线性系数均大于0.99;在癌性胸水小鼠模型中,各脏器硫化氢含量随着注射癌细胞数量的增多均有不同程度的增加趋势。结论该方法操作简单、成本低、灵敏度高、特异性强,适合各类组织样本的高通量检测,可为硫化氢相关药效机制的深入研究和相关药物开发提供方法学参考。展开更多
Compared with other transition metal Mxene derived quantum dots(MQD_(S)),Ta-based Mxene quantum dots have good functionality,but Ta-based Mxene quantum dots and their applications have not been studied so far.In this ...Compared with other transition metal Mxene derived quantum dots(MQD_(S)),Ta-based Mxene quantum dots have good functionality,but Ta-based Mxene quantum dots and their applications have not been studied so far.In this paper,we report for the first time the synthesis of high fluorescence quantum yield(QY) N-doped Ta_(4)C_(3) quantum dots(N-MQDs) using Ta_(4)C_(3) quantum dots in acid reflux damaged Ta_(4)C_(3) nanosheets as precursors and ethylenediamine as nitrogen source.The prepared N-MQDs have excellent blue photoluminescence(PL) properties,particle size is only 2.60 nm,QY is up to 23.4%,and good stability.In addition,it has been reported that N-MQDs can be used as fluorescent probe for detection of Fe;and remote force sensing analysis In liquid ion sensing,N-MQDS shows a unique selective quenching of Fe;with a detection limit as low as 2 μmol/L,and has great potential as a fast and super-sensitive fluorescent probe for the detection of heavy ion.More importantly,in solid mechanics sensing,the introduction of N-MQDs into self-healing hydrogels can be developed into a fluorescent hydrogel that can be used for accurate remote force measurement and applied in the field of mechanical sensing analysis.Therefore,Ta-based N-MQDs show excellent potential in the field of fluorescence sensing,which provides a door for multi-dimensional sensing of new materials in the future.展开更多
Drug-induced liver injury(DILI)is a common and serious adverse drug reaction.At present,DILI is perfectly diagnozed in clinical settings using Roussel Uclaf causality assessment method(RUCAM)in its original version pu...Drug-induced liver injury(DILI)is a common and serious adverse drug reaction.At present,DILI is perfectly diagnozed in clinical settings using Roussel Uclaf causality assessment method(RUCAM)in its original version published 1993 and its updated version published 2016,well established worldwide as a diagnostic algorithm with a high sensitivity and specificity.Nevertheless,the search for additional detection methods supporting RUCAM continues.In recent years,with the development of optical imaging technology,fluorescent probes have gradually shown great advantages in the detection and diagnosis of DILI markers such as high sensitivity,anti-interference,real-time monitoring and non-invasive measurement.In this review,the recent advances of fluorescent probes for evaluation of DILI in experimental studies were summarized according to various markers of DILI.We believe that learning about the design and practical application of these probes will contribute to the further development of detection sensors for DILI markers.展开更多
文摘目的快速制备并分离纯化硫化氢特异性荧光探针(Washington State Probe-5),建立动物组织中硫化氢的荧光探针测定法,并在癌性胸水模型中进行方法适用性验证。方法优化条件分离纯化WSP-5;对荧光探针反应液配制溶剂、DMSO加入体积、pH、反应液溶剂和反应液体积、样品前处理温度、研磨次数、研磨后静置时间等条件进行考察;以S-180腹水瘤细胞建立癌性胸水小鼠模型,测定小鼠各脏器中的硫化氢含量。结果以硅胶和葡聚糖凝胶为固定相,二氯甲烷-甲醇-甲酸(60∶1∶0.1,V/V/V)和二氯甲烷-甲醇(1∶1,V/V)为洗脱剂制备WSP-5纯品;动物组织样本和硫氢化钠标准溶液加入优化后的5倍量冰冷反应液,低温研磨、高速离心,上清液避光孵育12 h,测定荧光强度并计算硫化氢浓度;该方法检测限约0.6μmol·L^(-1),定量限1μmol·L^(-1),在硫氢化钠一定浓度范围内,浓度与荧光强度线性系数均大于0.99;在癌性胸水小鼠模型中,各脏器硫化氢含量随着注射癌细胞数量的增多均有不同程度的增加趋势。结论该方法操作简单、成本低、灵敏度高、特异性强,适合各类组织样本的高通量检测,可为硫化氢相关药效机制的深入研究和相关药物开发提供方法学参考。
基金supported by the National Natural Science Foundation of China (No. 81972901)the Key R&D Plan of Chenzhou (No. ZDYF202008)+1 种基金the Discipline Leader Startup Fund of Huazhong University of Science and Technology Union Shenzhen Hospital (No. YN2021002)Science Foundation of China University of Petroleum,Beijing (Nos. 2462019QNXZ02, 2462019BJRC007)。
文摘Compared with other transition metal Mxene derived quantum dots(MQD_(S)),Ta-based Mxene quantum dots have good functionality,but Ta-based Mxene quantum dots and their applications have not been studied so far.In this paper,we report for the first time the synthesis of high fluorescence quantum yield(QY) N-doped Ta_(4)C_(3) quantum dots(N-MQDs) using Ta_(4)C_(3) quantum dots in acid reflux damaged Ta_(4)C_(3) nanosheets as precursors and ethylenediamine as nitrogen source.The prepared N-MQDs have excellent blue photoluminescence(PL) properties,particle size is only 2.60 nm,QY is up to 23.4%,and good stability.In addition,it has been reported that N-MQDs can be used as fluorescent probe for detection of Fe;and remote force sensing analysis In liquid ion sensing,N-MQDS shows a unique selective quenching of Fe;with a detection limit as low as 2 μmol/L,and has great potential as a fast and super-sensitive fluorescent probe for the detection of heavy ion.More importantly,in solid mechanics sensing,the introduction of N-MQDs into self-healing hydrogels can be developed into a fluorescent hydrogel that can be used for accurate remote force measurement and applied in the field of mechanical sensing analysis.Therefore,Ta-based N-MQDs show excellent potential in the field of fluorescence sensing,which provides a door for multi-dimensional sensing of new materials in the future.
基金supported by National Natural Science Foundationof China(No.U1804136)。
文摘Drug-induced liver injury(DILI)is a common and serious adverse drug reaction.At present,DILI is perfectly diagnozed in clinical settings using Roussel Uclaf causality assessment method(RUCAM)in its original version published 1993 and its updated version published 2016,well established worldwide as a diagnostic algorithm with a high sensitivity and specificity.Nevertheless,the search for additional detection methods supporting RUCAM continues.In recent years,with the development of optical imaging technology,fluorescent probes have gradually shown great advantages in the detection and diagnosis of DILI markers such as high sensitivity,anti-interference,real-time monitoring and non-invasive measurement.In this review,the recent advances of fluorescent probes for evaluation of DILI in experimental studies were summarized according to various markers of DILI.We believe that learning about the design and practical application of these probes will contribute to the further development of detection sensors for DILI markers.
