Pancreatic ductal adenocarcinoma(PDAC)is universally acknowledged as the cancer with the highest mortality rate.Berberine has high medicinal value and has been used as an anti-cancer agent.Hence the purpose of this st...Pancreatic ductal adenocarcinoma(PDAC)is universally acknowledged as the cancer with the highest mortality rate.Berberine has high medicinal value and has been used as an anti-cancer agent.Hence the purpose of this study was to investigate the anti-cancer effect of berberine in PDAC.Berberine was shown to have a selective anti-cancer effect on PDAC by MTT assay in vitro.Pancreatic cancer stem cells(PCSCs),regulated by epithelial-mesenchymal transition(EMT),could promote the proliferation of PDAC cells.However,berberine suppressed the proliferation and stemness of PCSCs through immunofluorescence staining,stem cell sphere assays and so forth in vitro.In vivo,berberine reduced tumor size and decreased the expression levels of Ki67,a marker of cellular proliferation,in orthotopic pancreatic tumors.In addition,berberine inhibited the EMT signaling pathway by RT-PCR and Western blotting methods both in vitro and in vivo.Our study indicates that berberine inhibits the proliferation of PDAC cells both in vivo and in vitro.The mechanism of the anti-cancer effect of berberine likely involves the inhibition of EMT.Therefore,berberine may be a novel antineoplastic drug with clinical efficacy in PDAC.展开更多
To investigate the technique of sorting high-purity precartilaginous stem cells from rat＇s perichondrium, neonatal rat＇s perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast grow...To investigate the technique of sorting high-purity precartilaginous stem cells from rat＇s perichondrium, neonatal rat＇s perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 （anti-FGFR-3）, and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody, Purity of the sorted neural stem cells was found to be 93.0 %-99.0 %, with living cells amounting to 80 % -85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.展开更多
Bone marrow mesenchymal stem cells(BMSCs),periosteal stem cells(PSCs),and other bone stem cells originate from embryonic bone formation,but their function and stem cell characteristics such as proliferation ability an...Bone marrow mesenchymal stem cells(BMSCs),periosteal stem cells(PSCs),and other bone stem cells originate from embryonic bone formation,but their function and stem cell characteristics such as proliferation ability and differentiation ability change at different anatomical locations.Perichondral-derived stem cells(PCSCs)are more closely related to PSCs in origin and function,usually used to be studied together with PSCs as one type of stem cell.However,this leads to the ignoration of the PCSCs'characteristics.Since the anatomical locations of these two types of stem cells diverse,PCSCs should have some differences from PSCs.In this study,the PCSCs in the perichondrium surrounding the growth plate cartilage expressed CTSK and CD200 same as PSCs.However,when compared the stem cell characteristics of PCSCs with that of PSCs,PCSCs were more elongated than PSCs in morphology and have stronger self-renewal ability,as well as stronger chondrogenic and adipogenic differentiation potentials.This study revealed the stem cell characteristics of PCSCs distinguished from PSCs,which may indicate PCSCs and PSCs should not be treated as one type of cell to research in the future.展开更多
基金This research was supported by 81803024(National Natural Science Foundation of China)and DUT21LK23(Fundamental Research Funds for the Central Universities).
文摘Pancreatic ductal adenocarcinoma(PDAC)is universally acknowledged as the cancer with the highest mortality rate.Berberine has high medicinal value and has been used as an anti-cancer agent.Hence the purpose of this study was to investigate the anti-cancer effect of berberine in PDAC.Berberine was shown to have a selective anti-cancer effect on PDAC by MTT assay in vitro.Pancreatic cancer stem cells(PCSCs),regulated by epithelial-mesenchymal transition(EMT),could promote the proliferation of PDAC cells.However,berberine suppressed the proliferation and stemness of PCSCs through immunofluorescence staining,stem cell sphere assays and so forth in vitro.In vivo,berberine reduced tumor size and decreased the expression levels of Ki67,a marker of cellular proliferation,in orthotopic pancreatic tumors.In addition,berberine inhibited the EMT signaling pathway by RT-PCR and Western blotting methods both in vitro and in vivo.Our study indicates that berberine inhibits the proliferation of PDAC cells both in vivo and in vitro.The mechanism of the anti-cancer effect of berberine likely involves the inhibition of EMT.Therefore,berberine may be a novel antineoplastic drug with clinical efficacy in PDAC.
基金This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30170944).
文摘To investigate the technique of sorting high-purity precartilaginous stem cells from rat＇s perichondrium, neonatal rat＇s perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 （anti-FGFR-3）, and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody, Purity of the sorted neural stem cells was found to be 93.0 %-99.0 %, with living cells amounting to 80 % -85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.
基金This research was funded by the National Natural Science Foundation of China,grants number 11972068 and 12002026funded by the China Space Station Engineering Experiment Project,grants number HYZHXM01016.
文摘Bone marrow mesenchymal stem cells(BMSCs),periosteal stem cells(PSCs),and other bone stem cells originate from embryonic bone formation,but their function and stem cell characteristics such as proliferation ability and differentiation ability change at different anatomical locations.Perichondral-derived stem cells(PCSCs)are more closely related to PSCs in origin and function,usually used to be studied together with PSCs as one type of stem cell.However,this leads to the ignoration of the PCSCs'characteristics.Since the anatomical locations of these two types of stem cells diverse,PCSCs should have some differences from PSCs.In this study,the PCSCs in the perichondrium surrounding the growth plate cartilage expressed CTSK and CD200 same as PSCs.However,when compared the stem cell characteristics of PCSCs with that of PSCs,PCSCs were more elongated than PSCs in morphology and have stronger self-renewal ability,as well as stronger chondrogenic and adipogenic differentiation potentials.This study revealed the stem cell characteristics of PCSCs distinguished from PSCs,which may indicate PCSCs and PSCs should not be treated as one type of cell to research in the future.
