The PCV-2 positive tissues by PCR-restriction fragment length polymorphism (PCR-RFLP) were inoculated to the PK-15 cells not contaminated by PCV. The viral particles were observed in the infected PK-15 cells by eletro...The PCV-2 positive tissues by PCR-restriction fragment length polymorphism (PCR-RFLP) were inoculated to the PK-15 cells not contaminated by PCV. The viral particles were observed in the infected PK-15 cells by eletronmicroscope .It was concluded that the isolate was PCV-2 and was named SCH-A strain.The capsid protein gene(cp) in ORF2 was amplified from PK-15 cell line infected with SCH-A strain by PCR, and was cloned and sequenced.The nucleotide sequences of ORF2 gene of the SCH-A strain was compared with other porcine circovirus isolates.The results showed that the homologies of nucleotides were between 90.5% and 96.7% in comparision with the PCV-2 reference strains,but the homologies of nucleotides were only about 67% with the PCV-1 reference strains. SCH-A strain displayed the highest nucleotide homology((96.7)%) with the reference strain Hunan .Phylogenetic tree analysis showed ORF2 gene of the SCH-A isolate belongs to PCV-2 .展开更多
根据已发表的猪2型圆环病毒(Porcine circovirus type 2,PCV-2)全基因组序列设计2对引物,用PCR的方法分2段克隆圆环病毒的基因,测序后拼接得到该圆环病毒的基因组。序列分析结果表明,PCV-SHZ基因组全长为1767bp(Genbank登录号:JF718784)...根据已发表的猪2型圆环病毒(Porcine circovirus type 2,PCV-2)全基因组序列设计2对引物,用PCR的方法分2段克隆圆环病毒的基因,测序后拼接得到该圆环病毒的基因组。序列分析结果表明,PCV-SHZ基因组全长为1767bp(Genbank登录号:JF718784),基因型为PCV-2a。用DNAMAN软件将此株病毒基因组与国内其它12个省市PCV-2参考株的基因组核苷酸序列进行同源性比较,同源率为95.25%～99.55%。用MEGA软件分别对该病毒和参考株的全基因组、ORF1和ORF2进行进化树分析,结果表明PCV-2流行株在进化上存在地域上的相关性。展开更多
文摘The PCV-2 positive tissues by PCR-restriction fragment length polymorphism (PCR-RFLP) were inoculated to the PK-15 cells not contaminated by PCV. The viral particles were observed in the infected PK-15 cells by eletronmicroscope .It was concluded that the isolate was PCV-2 and was named SCH-A strain.The capsid protein gene(cp) in ORF2 was amplified from PK-15 cell line infected with SCH-A strain by PCR, and was cloned and sequenced.The nucleotide sequences of ORF2 gene of the SCH-A strain was compared with other porcine circovirus isolates.The results showed that the homologies of nucleotides were between 90.5% and 96.7% in comparision with the PCV-2 reference strains,but the homologies of nucleotides were only about 67% with the PCV-1 reference strains. SCH-A strain displayed the highest nucleotide homology((96.7)%) with the reference strain Hunan .Phylogenetic tree analysis showed ORF2 gene of the SCH-A isolate belongs to PCV-2 .
文摘根据已发表的猪2型圆环病毒(Porcine circovirus type 2,PCV-2)全基因组序列设计2对引物,用PCR的方法分2段克隆圆环病毒的基因,测序后拼接得到该圆环病毒的基因组。序列分析结果表明,PCV-SHZ基因组全长为1767bp(Genbank登录号:JF718784),基因型为PCV-2a。用DNAMAN软件将此株病毒基因组与国内其它12个省市PCV-2参考株的基因组核苷酸序列进行同源性比较,同源率为95.25%～99.55%。用MEGA软件分别对该病毒和参考株的全基因组、ORF1和ORF2进行进化树分析,结果表明PCV-2流行株在进化上存在地域上的相关性。
