Objective The aim of the article is to explore the function of circBAGE2(hsa_circ_0061259)in prostate cancer(PCa)cells.Methods Sequencing results of circBAGE2 were verified by quantitative RT PCR(qRT-PCR)and Sanger se...Objective The aim of the article is to explore the function of circBAGE2(hsa_circ_0061259)in prostate cancer(PCa)cells.Methods Sequencing results of circBAGE2 were verified by quantitative RT PCR(qRT-PCR)and Sanger sequencing.Agarose gel electrophoresis was used to detect the resistance of GAPDH,BAGE2,and circBAGE2 to RNase R and their expression as cDNA and gDNAin 22RV1 cells.The biological functions of circBAGE2 were investigated by CCK8 assay and flow cytometry in 22RV1 cells transfected with siRNAs.Multiple databases were used to predict the target binding sites between circRNAs,miRNAs,and mRNAs.Western blotting was used to detect the expression of CCND1 and PDCD10.Results CircBAGE2 was significantly upregulated in PCa samples and PCa cells compared to that in matched normal tissues and normal cells,and CircBAGE2 knockdown inhibits cell proliferation and promotes apoptosis.Downregulation of circBAGE2 compromised the expression of CCND1 and PDCD10.The 3’UTRs of CCND1 and PDCD10 were matched by miR-103a-3p,which shared binding sites with circBAGE2.Conclusion CircBAGE2 contributes to PCa progression by upregulating CCND1 and PDCD10 expression through its role as a‘sponge’of miR-103a-3p.CircBAGE2 may be a potential therapeutic target for PCa.展开更多
The programmed cell death 10(PDCD10)gene was originally identified as an apoptosis-related gene,although it is now usually known as CCM3,as the third causative gene of cerebral cavernous malformation(CCM).CCM is a neu...The programmed cell death 10(PDCD10)gene was originally identified as an apoptosis-related gene,although it is now usually known as CCM3,as the third causative gene of cerebral cavernous malformation(CCM).CCM is a neurovascular disease that is characterized by vascular malformations and is associated with headaches,seizures,focal neurological deficits,and cerebral hemorrhage.The PDCD10/CCM3 protein has multiple subcellular localizations and interacts with several multi-protein complexes and signaling pathways.Thus PDCD10/CCM3 governs many cellular functions,which include cell-to-cell junctions and cytoskeleton organization,cell proliferation and apoptosis,and exocytosis and angiogenesis.Given its central role in the maintenance of homeostasis of the cell,dysregulation of PDCD10/CCM3 can result in a wide range of altered cell functions.This can lead to severe diseases,including CCM,cognitive disability,and several types of cancers.Here,we review the multifaceted roles of PDCD10/CCM3 in physiology and pathology,with a focus on its functions beyond CCM.展开更多
基金Supported by grants from the Natural Science Foundation of Gansu Province(No.20JR5RA601)In-hospital Project of The 940 Hospital of Joint Logistics Support Force of Chinese PLA(No.2021yxky057).
文摘Objective The aim of the article is to explore the function of circBAGE2(hsa_circ_0061259)in prostate cancer(PCa)cells.Methods Sequencing results of circBAGE2 were verified by quantitative RT PCR(qRT-PCR)and Sanger sequencing.Agarose gel electrophoresis was used to detect the resistance of GAPDH,BAGE2,and circBAGE2 to RNase R and their expression as cDNA and gDNAin 22RV1 cells.The biological functions of circBAGE2 were investigated by CCK8 assay and flow cytometry in 22RV1 cells transfected with siRNAs.Multiple databases were used to predict the target binding sites between circRNAs,miRNAs,and mRNAs.Western blotting was used to detect the expression of CCND1 and PDCD10.Results CircBAGE2 was significantly upregulated in PCa samples and PCa cells compared to that in matched normal tissues and normal cells,and CircBAGE2 knockdown inhibits cell proliferation and promotes apoptosis.Downregulation of circBAGE2 compromised the expression of CCND1 and PDCD10.The 3’UTRs of CCND1 and PDCD10 were matched by miR-103a-3p,which shared binding sites with circBAGE2.Conclusion CircBAGE2 contributes to PCa progression by upregulating CCND1 and PDCD10 expression through its role as a‘sponge’of miR-103a-3p.CircBAGE2 may be a potential therapeutic target for PCa.
基金The lab of ED is supported by:The Swedish Research Council(contract No.2013-9279),the Knut and Alice Wallenberg Foundation(contract No.2015-0030),the European Research Council(project EC-ERC-VEPC,contract 74292),Associazione Italiana per la Ricerca sul Cancro(AIRC IG 23223),AIRC 5x1000 call"Metastatic disease:the key unmet need in oncology"to the MYNERVA Project(MYeloid NEoplasms Research Venture AIRC 21267),Telethon(New insight on the pathogenesis of hereditary Cerebral Cavernous Malformation,contract GGP19202)AIFA(a multicellular randomized clinical trial on propranolol in familial Cerebral Cavernous Malformation,contract AIFA-2016-02364593).Be Brave for Life Foundation Micro Grant 2019.
文摘The programmed cell death 10(PDCD10)gene was originally identified as an apoptosis-related gene,although it is now usually known as CCM3,as the third causative gene of cerebral cavernous malformation(CCM).CCM is a neurovascular disease that is characterized by vascular malformations and is associated with headaches,seizures,focal neurological deficits,and cerebral hemorrhage.The PDCD10/CCM3 protein has multiple subcellular localizations and interacts with several multi-protein complexes and signaling pathways.Thus PDCD10/CCM3 governs many cellular functions,which include cell-to-cell junctions and cytoskeleton organization,cell proliferation and apoptosis,and exocytosis and angiogenesis.Given its central role in the maintenance of homeostasis of the cell,dysregulation of PDCD10/CCM3 can result in a wide range of altered cell functions.This can lead to severe diseases,including CCM,cognitive disability,and several types of cancers.Here,we review the multifaceted roles of PDCD10/CCM3 in physiology and pathology,with a focus on its functions beyond CCM.
