Phosphatidylcholine(PC)and phosphatidylethanolatmine(PE)are successfully separated,meanwhile various components of phosphatidylcholines can also be separated by using the following operating conditions:the Lichrosorb ...Phosphatidylcholine(PC)and phosphatidylethanolatmine(PE)are successfully separated,meanwhile various components of phosphatidylcholines can also be separated by using the following operating conditions:the Lichrosorb Si 60 column(4.6mm×250mmi.d.5μm),hexane:iso-propanol:water:85%phosphate(45:48:7.5:0.15,volume ratio)as mobile phase,40℃,1.0mL/min flow rate and UV detection at 206 nm.This method is very simple,and its recovery and accuracy are very high,so it is especially suitable for the preliminary analysis of phosphatidylcholine components.展开更多
为了降低天然大分子功能蛋白的分离纯化成本,以琼脂为原料自制弱阴离子交换层析介质DEAE-琼脂凝胶微球。将DEAE-琼脂凝胶微球应用于坛紫菜R-藻红蛋白（R-PE）和螺旋藻C-藻蓝蛋白（C-PC）的提取分离,最佳洗脱条件为：层析柱规格h=35.0 cm...为了降低天然大分子功能蛋白的分离纯化成本,以琼脂为原料自制弱阴离子交换层析介质DEAE-琼脂凝胶微球。将DEAE-琼脂凝胶微球应用于坛紫菜R-藻红蛋白（R-PE）和螺旋藻C-藻蓝蛋白（C-PC）的提取分离,最佳洗脱条件为：层析柱规格h=35.0 cm,φ=1.0 cm,V=27.5 m L;流速v=0.33 m L/min;洗脱缓冲液为0.01 mol/L磷酸盐溶液（含有0.05~0.5 mol/L Na Cl溶液）;0.01 mol/L磷酸盐缓冲液（含0.1 mol/L Na Cl）洗脱出纯度为5.0的坛紫菜R-PE;0.01 mol/L磷酸盐缓冲液（含0.2 mol/L Na Cl）洗脱出纯度为4.4的螺旋藻C-PC。自制层析介质成本低廉、耗能少、可大量制备、分离效果好,可用于坛紫菜R-PE、螺旋藻C-PC的产业化制备。展开更多
文摘Phosphatidylcholine(PC)and phosphatidylethanolatmine(PE)are successfully separated,meanwhile various components of phosphatidylcholines can also be separated by using the following operating conditions:the Lichrosorb Si 60 column(4.6mm×250mmi.d.5μm),hexane:iso-propanol:water:85%phosphate(45:48:7.5:0.15,volume ratio)as mobile phase,40℃,1.0mL/min flow rate and UV detection at 206 nm.This method is very simple,and its recovery and accuracy are very high,so it is especially suitable for the preliminary analysis of phosphatidylcholine components.
文摘为了降低天然大分子功能蛋白的分离纯化成本,以琼脂为原料自制弱阴离子交换层析介质DEAE-琼脂凝胶微球。将DEAE-琼脂凝胶微球应用于坛紫菜R-藻红蛋白（R-PE）和螺旋藻C-藻蓝蛋白（C-PC）的提取分离,最佳洗脱条件为：层析柱规格h=35.0 cm,φ=1.0 cm,V=27.5 m L;流速v=0.33 m L/min;洗脱缓冲液为0.01 mol/L磷酸盐溶液（含有0.05~0.5 mol/L Na Cl溶液）;0.01 mol/L磷酸盐缓冲液（含0.1 mol/L Na Cl）洗脱出纯度为5.0的坛紫菜R-PE;0.01 mol/L磷酸盐缓冲液（含0.2 mol/L Na Cl）洗脱出纯度为4.4的螺旋藻C-PC。自制层析介质成本低廉、耗能少、可大量制备、分离效果好,可用于坛紫菜R-PE、螺旋藻C-PC的产业化制备。
