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2. Materials and methods 2.1. Materials
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《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2013年第3期252-258,共7页
PEOl13-(hb-PG)ls-g-PCL22 (Mn = 8500) and PEO113- (hb-PG)Is-g-PCL5o (Mn=ll700) were kindly donated by Zhenlin Zhong in the College of Chemistry and Molecular Sciences, Wuhan University. Fluorescent probes inclu... PEOl13-(hb-PG)ls-g-PCL22 (Mn = 8500) and PEO113- (hb-PG)Is-g-PCL5o (Mn=ll700) were kindly donated by Zhenlin Zhong in the College of Chemistry and Molecular Sciences, Wuhan University. Fluorescent probes including rhodamine-phalloidin (RP) and Hoechst 33258 were the products of Molecular Probes Inc. (Eugene, OR, USA). Coumarin-6 (C6), and inhibitors such as methyl-13- cyclodextrin (MI3CD), filipin and phenylarsine oxide (PAO) were all purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM), penicillin-streptomycin and fetal bovine serum (FBS) were from GIBCO, Invitrogen Corp. (Carlsbad, CA, USA). Transwells (12 wells, pore diameter of 3 gm, polycarbonate) were supplied by Coming Costar (Cambridge, MA). All other reagents were of analytical grade and came from commercial sources. 展开更多
关键词 包载香豆素-6 peo-(hb-pg)-g-pcl 杂臂取合物胶束 细胞提取 内吞
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