Protective Effects and Action Mechanism of Total Flavonoids of POLYGONI PERFOLIATI HERBA on Rats with Nonalcoholic Steatohepatitis

[Objectives]To study the protective effects and mechanism of POLYGONI PERFOLIATI HERBA on rats with nonalcoholic steatohepatitis( NASH). [Methods] Sixty rats were randomly divided into normal group,model group,metformin group( 0. 5 mg/kg),and high dose,medium dose,and low dose groups of total flavonoids of POLYGONI PERFOLIATI HERBA( 600,300,150 mg/kg). The standard feed was given to the normal group,and the model group and the total flavonoids groups were fed with high-fat diet for 13 weeks to establish the NASH rat model. At the 8 th week,the metformin group and the POLYGONI PERFOLIATI HERBA total flavonoids groups were given with the corresponding drug treatment for 6 weeks,blood was taken from the eyeball to collect liver tissue. Biochemical method was used to determine ALT,AST,TC,TG,HDL-c,LDL-c,SOD,MDA,GSH-Px activity or content in serum and HOMA-IR,and ELISA was used to measure the TNF-α,IL-1β and IL-6 content in liver tissue; Western blotting was used to detect the expression levels of AMPK,p-AMPK and ACC in liver tissue. [Results] Total flavonoids of POLYGONI PERFOLIATI HERBA could significantly decrease the activity or content of ALT,AST and MDA in serum of NASH rats( P < 0. 05,P < 0. 01),and enhance the activity of SOD and GSH-Px( P < 0. 05,P < 0. 01),reduce serum TC,TG,LDL-c levels and insulin resistance index,increase HDL-c levels( P < 0. 05,P < 0. 01); down-regulate liver IL-1β,IL-6,TNF-α,ACC levels and up-regulate p-AMPK expression( P < 0. 05,P < 0. 01). [Conclusions]The total flavonoids of POLYGONI PERFOLIATI HERBA have a good protective effect on NASH rats,and its mechanism may be related to the functions of regulating the lipid metabolism,alleviating insulin resistance,inhibiting oxidative stress,inhibiting inflammatory reaction and regulating AMPK and ACC protein expression.

Research Overview of Zhuang Medicine Fumigation Lotions

At present,there is little research on the application of Zhuang medicine fumigation lotions in skin diseases,and there is no research on related preparation processes and quality standards.In this paper,the characteristics of its formulation and the research progress in skin diseases are reviewed,in order to deepen the understanding of Zhuang medicine fumigation lotions and provide a more detailed theoretical basis for its clinical research,development and application.

壮药杠板归研究概况

杠板归为蓼科(Polygonaceae)植物杠板归(Polygonum perfoliatum L.)的干燥地上部分,具有清热解毒、利水消肿、祛痰止咳、散瘀止血的功效。杠板归中含有黄酮、蒽醌、萜类、生物碱等多种化合物,黄酮类是其主要化学成分。现代研究表明,杠板归具有抗病毒、抗炎、抗肿瘤等多种药理作用,药用价值极高,被广泛应用于临床。作者整理了国内外关于杠板归的研究报道,对杠板归在鉴定、化学成分、药理作用、临床应用方面的研究进展进行综述,为其进一步研究及开发利用提供一定参考。

HPLC法测定杠板归中槲皮素的含量

目的:建立草药杠板归中槲皮素含量的测定方法。方法:采用高效液相色谱法,色谱柱为AE.Lichrom C_(18)柱(150mm×4.6 mm,5μm),流动相为甲醇-0.4%磷酸(50:50),流速为1.0 ml·min^(-1),检测波长为360 nm。结果:槲皮素的进样量在0.012～0.616μg范围内与峰面积线形关系良好(r=0.999 9),平均回收率为98.64%(RSD=0.83%)。结论:此法操作简单,结果可靠,可用于杠板归的质量控制。

蜜制杠板归对昆明小鼠的镇咳实验研究

目的研究杠板归蜜制后镇咳作用。方法采用浓氨水致昆明小鼠咳嗽实验,观察记录对照组、生药组、炮制组与阳性组小鼠咳嗽潜伏期及咳嗽开始后3min内的咳嗽次数,利用SPSS 19.0对数据进行处理,研究蜜制杠板归对镇咳作用的影响。结果杠板归蜜制后,其对浓氨水刺激小鼠咳嗽潜伏期明显延长,开始咳嗽后3min内的咳嗽次数明显减少。结论杠板归蜜制炮制后镇咳作用明显提高。

基于层次分析-熵权法和指纹图谱的杠板归质量标志物研究

基于层次分析(analytic hierarchy process, AHP)-熵权法(entropy weight method, EWM)、网络药理学、谱效关系法研究杠板归潜在的质量标志物(Q-marker)。应用AHP-EWM对杠板归的质量标志物进行量化辨识,AHP应用于一级指标有效性、可测性和特有性的权重分析,EWM分析各项下有文献及实验数据支撑的二级指标。有效性分析基于文献和网络药理学方法,对杠板归的“成分-靶点-疾病-功效”网络关系进行研究,筛选出与清热解毒、利水消肿、止咳功效关联性最强的成分;可测性分析基于文献研究和HPLC同时测定杠板归中的10个成分,建立杠板归的指纹图谱;特有性分析通过统计杠板归的成分存在于多少种植物中。整合分析确定槲皮素、齐墩果酸、鞣花酸、没食子酸、山柰酚、芦丁、七叶内酯、槲皮素-3-葡萄糖醛酸苷、熊果酸、原儿茶酸、阿魏酸共11个化合物作为杠板归的潜在质量标志物。通过抗炎活性验证,杠板归中11个化合物具有良好的抗炎活性,说明杠板归11个质量标志物可能是杠板归的功效物质基础。该研究以杠板归为研究对象,基于多层次法研究杠板归的质量标志物,为杠板归的质量控制提供参考依据。