为估价心肌显像在 X 综合征诊断中的意义,用^(99m)Tc-甲氧基异丁基异腈对21例有典型心绞痛、冠状动脉造影正常、运动试验心电图阳性而诊断为 X 综合征的患者进行了心肌 SPECT 显像,并与17例临床有胸痛、冠状动脉造影正常、运动试验心电...为估价心肌显像在 X 综合征诊断中的意义,用^(99m)Tc-甲氧基异丁基异腈对21例有典型心绞痛、冠状动脉造影正常、运动试验心电图阳性而诊断为 X 综合征的患者进行了心肌 SPECT 显像,并与17例临床有胸痛、冠状动脉造影正常、运动试验心电图阴性的非 X 综合征患者和26例冠状动脉造影证实为冠心病心绞痛患者的心肌显像进行对比。结果:21例 X 综合征患者经心肌 SPECT 检查11例有心肌缺血,其心肌缺血半定量计分为1.1±0.3,而冠心病心绞痛者心肌缺血计分为1.8±0.7(t=3.174 6,P<0.01)。故心肌 SPECT 显像可检出部分 X 综合征的心肌缺血,其缺血程度比冠心病心绞痛者轻。展开更多
To establish a method to evaluate the effects of chemosensitizer onP-glycoprotein using ^(99m)Tc-MIBI, and observe the changes of ^(99m)Tc-MIBI uptake kinetics andP-glycoprotein levels after using verapamil in MDR hum...To establish a method to evaluate the effects of chemosensitizer onP-glycoprotein using ^(99m)Tc-MIBI, and observe the changes of ^(99m)Tc-MIBI uptake kinetics andP-glycoprotein levels after using verapamil in MDR human breast cells MCF-7/Adr. Methods: MDR breastcarcinoma cells, MCF-7/Adr, were incubated and different protocols were performed. Protocol Ⅰ: achemosensitizer, verapamil (10 μmol/L), was added into cell culture medium, while in control group,the same volume of DMEM was given. Cells were harvested after 2 h incubation with ^(99m)Tc-MIBI.Protocol Ⅱ: Verapamil (10 μmol/L) was added into cell culture medium and incubated for 20 min, 40min, 60 min, 80 min, 8 h, 24 h, 48 h and 72 h respectively. Cells were harvested after 2 hincubation with ^(99m)Tc-MIBI. The radioactivity of the cells was measured and P-glycoproteinexpression levels were determined with immunohistochemical stain. Results: Protocol Ⅰ: After 2hincubation with verapamil the cellular uptake of ^(99m)Tc-MIBI was remarkably higher than controlgroup (t=2.33, P 【 0.05), but there was no difference in P-glycoprotein expression levels betweentwo groups (P 】 0.05). Protocol Ⅱ: In verapamil group, ^(99m)Tc-MIBI uptake was increased withincubation time prolonging (F=58.2, P 【 0.05). When verapamil incubation time surpassed 8 h the^(99m)Tc-MIBI uptake negatively correlated to the P-glycoprotein expression levels (r=-0.73, P 【0.01). However, when incubation time was less than 80 min, there was no correlation between^(99m)Tc-MIBI accumulation and P-glycoprotein levels (r=0.16, P 】 0.05). Conclusion: ^(99m)Tc-MIBImay be used to evaluate the qualitative as well as quantitative change of P-glycoprotein expressionlevels induced by the chemosensitizer, verapamil.展开更多
文摘为估价心肌显像在 X 综合征诊断中的意义,用^(99m)Tc-甲氧基异丁基异腈对21例有典型心绞痛、冠状动脉造影正常、运动试验心电图阳性而诊断为 X 综合征的患者进行了心肌 SPECT 显像,并与17例临床有胸痛、冠状动脉造影正常、运动试验心电图阴性的非 X 综合征患者和26例冠状动脉造影证实为冠心病心绞痛患者的心肌显像进行对比。结果:21例 X 综合征患者经心肌 SPECT 检查11例有心肌缺血,其心肌缺血半定量计分为1.1±0.3,而冠心病心绞痛者心肌缺血计分为1.8±0.7(t=3.174 6,P<0.01)。故心肌 SPECT 显像可检出部分 X 综合征的心肌缺血,其缺血程度比冠心病心绞痛者轻。
文摘To establish a method to evaluate the effects of chemosensitizer onP-glycoprotein using ^(99m)Tc-MIBI, and observe the changes of ^(99m)Tc-MIBI uptake kinetics andP-glycoprotein levels after using verapamil in MDR human breast cells MCF-7/Adr. Methods: MDR breastcarcinoma cells, MCF-7/Adr, were incubated and different protocols were performed. Protocol Ⅰ: achemosensitizer, verapamil (10 μmol/L), was added into cell culture medium, while in control group,the same volume of DMEM was given. Cells were harvested after 2 h incubation with ^(99m)Tc-MIBI.Protocol Ⅱ: Verapamil (10 μmol/L) was added into cell culture medium and incubated for 20 min, 40min, 60 min, 80 min, 8 h, 24 h, 48 h and 72 h respectively. Cells were harvested after 2 hincubation with ^(99m)Tc-MIBI. The radioactivity of the cells was measured and P-glycoproteinexpression levels were determined with immunohistochemical stain. Results: Protocol Ⅰ: After 2hincubation with verapamil the cellular uptake of ^(99m)Tc-MIBI was remarkably higher than controlgroup (t=2.33, P 【 0.05), but there was no difference in P-glycoprotein expression levels betweentwo groups (P 】 0.05). Protocol Ⅱ: In verapamil group, ^(99m)Tc-MIBI uptake was increased withincubation time prolonging (F=58.2, P 【 0.05). When verapamil incubation time surpassed 8 h the^(99m)Tc-MIBI uptake negatively correlated to the P-glycoprotein expression levels (r=-0.73, P 【0.01). However, when incubation time was less than 80 min, there was no correlation between^(99m)Tc-MIBI accumulation and P-glycoprotein levels (r=0.16, P 】 0.05). Conclusion: ^(99m)Tc-MIBImay be used to evaluate the qualitative as well as quantitative change of P-glycoprotein expressionlevels induced by the chemosensitizer, verapamil.
