提出一种基于水溶性共轭聚合物PFP荧光共振能量转移原理检测氧化损伤DNA的方法。通过加入DNA修复酶来识别并切除被氧化的DNA碱基,获得含磷酸基团核苷酸空隙的DNA,再通过羟基引入荧光标记物,加入PFP后可得到PFPDNA复合物,通过荧光共振能...提出一种基于水溶性共轭聚合物PFP荧光共振能量转移原理检测氧化损伤DNA的方法。通过加入DNA修复酶来识别并切除被氧化的DNA碱基,获得含磷酸基团核苷酸空隙的DNA,再通过羟基引入荧光标记物,加入PFP后可得到PFPDNA复合物,通过荧光共振能量转移(fluorescence resonance energy transfer,FRET)进行氧化损伤DNA的检测。对方法的影响因素进行了考查和优化,包括PFP的浓度、DNA修复酶的种类、DNA聚合酶I的用量以及芬顿反应的时间等。研究表明,基于PFP荧光共振能量转移原理检测氧化损伤DNA方法具有灵敏度高和特异性强的优势,可确保检测的准确性,对老年疾病的预防医学具有很好的应用前景。展开更多
Patellofemoral pain syndrome (PFPS) is a common musculoskeletal condition, with a high incidence in young active adolescents, as well as in other age groups. The multifactorial etiology of the syndrome makes the clini...Patellofemoral pain syndrome (PFPS) is a common musculoskeletal condition, with a high incidence in young active adolescents, as well as in other age groups. The multifactorial etiology of the syndrome makes the clinical evaluation and synthesis of an effective treatment plan particularly demanding and difficult. The absence of a specific clinical trial for the diagnosis or rejection of PFPS leads to the diagnosis of the syndrome through the careful rejection of other conditions with similar symptoms. Physiotherapy is based on the management and immediate treatment of the patient’s pain, by combining active and passive means of treatment and address the fear of exercise and movement. Interventions for the treatment of patellofemoral pain present positive short-term but not as encouraging long-term results. Therefore we are posed with an interesting question: what is the most effective treatment plan for the management of PFPS? The purpose of the review is to present the necessary and most up-to-date data for the identification, clinical evaluation and etiology of the syndrome as well as to compose in the form of guidelines (Guidelines) the basis for building an effective treatment plan for the treatment of PFPS.展开更多
文摘提出一种基于水溶性共轭聚合物PFP荧光共振能量转移原理检测氧化损伤DNA的方法。通过加入DNA修复酶来识别并切除被氧化的DNA碱基,获得含磷酸基团核苷酸空隙的DNA,再通过羟基引入荧光标记物,加入PFP后可得到PFPDNA复合物,通过荧光共振能量转移(fluorescence resonance energy transfer,FRET)进行氧化损伤DNA的检测。对方法的影响因素进行了考查和优化,包括PFP的浓度、DNA修复酶的种类、DNA聚合酶I的用量以及芬顿反应的时间等。研究表明,基于PFP荧光共振能量转移原理检测氧化损伤DNA方法具有灵敏度高和特异性强的优势,可确保检测的准确性,对老年疾病的预防医学具有很好的应用前景。
文摘Patellofemoral pain syndrome (PFPS) is a common musculoskeletal condition, with a high incidence in young active adolescents, as well as in other age groups. The multifactorial etiology of the syndrome makes the clinical evaluation and synthesis of an effective treatment plan particularly demanding and difficult. The absence of a specific clinical trial for the diagnosis or rejection of PFPS leads to the diagnosis of the syndrome through the careful rejection of other conditions with similar symptoms. Physiotherapy is based on the management and immediate treatment of the patient’s pain, by combining active and passive means of treatment and address the fear of exercise and movement. Interventions for the treatment of patellofemoral pain present positive short-term but not as encouraging long-term results. Therefore we are posed with an interesting question: what is the most effective treatment plan for the management of PFPS? The purpose of the review is to present the necessary and most up-to-date data for the identification, clinical evaluation and etiology of the syndrome as well as to compose in the form of guidelines (Guidelines) the basis for building an effective treatment plan for the treatment of PFPS.
