Prototype foamy virus(PFV)is a unique retrovirus that infects animals and humans and does not cause clinical symptoms.Long noncoding RNAs(lncRNAs)are believed to exert multiple regulatory functions during viral infect...Prototype foamy virus(PFV)is a unique retrovirus that infects animals and humans and does not cause clinical symptoms.Long noncoding RNAs(lncRNAs)are believed to exert multiple regulatory functions during viral infections.Previously,we utilized RNA sequencing(RNA-seq)to characterize and identify the lncRNA lnc-RP5-1086 D14.3.1-1:1(lnc-RP5),which is markedly decreased in PFV-infected cells.However,little is known about the function of lnc-RP5 during PFV infection.In this study,we identified lnc-RP5 as a regulator of the PFV transcriptional transactivator(Tas).Lnc-RP5 enhanced the activity of the PFV internal promoter(IP).The expression of PFV Tas was found to be promoted by lnc-RP5.Moreover,mi R-129-5 p was found to be involved in the lnc-RP5-mediated promotion of PFV IP activity,while the Notch1 protein suppressed the activity of PFV IP and the expression of Tas.Our results demonstrate that lnc-RP5 promotes the expression of PFV Tas through the miR-129-5 p/Notch1/PFV IP axis.This work provides evidence that host lnc RNAs can manipulate PFV replication by employing mi RNAs and proteins during an early viral infection.展开更多
电化学作为现代仪器分析重要的组成部分,在生命科学领域正扮演着越来越重要的角色.随着蛋白膜伏安法(protein film voltammetery,PFV)的不断完善,以及表面修饰技术、纳米技术、分子标记与分子识别技术等相关技术的不断发展,在蛋白酶研...电化学作为现代仪器分析重要的组成部分,在生命科学领域正扮演着越来越重要的角色.随着蛋白膜伏安法(protein film voltammetery,PFV)的不断完善,以及表面修饰技术、纳米技术、分子标记与分子识别技术等相关技术的不断发展,在蛋白酶研究领域,电化学正逐渐克服以往手段较为单一、研究目标不够多样、获取信息不够丰富等弊端.以本课题组在该领域所取得的一些研究成果为主,综述蛋白酶电化学研究的现状和发展趋势.展开更多
Potyviruses are plant viruses that infect various kinds of economic crops and cause enormous losses.Here w e identified three potyviruses,East Asian passiflora virus(EAPV),telosma mosaic virus(TelMV)and passion fruit ...Potyviruses are plant viruses that infect various kinds of economic crops and cause enormous losses.Here w e identified three potyviruses,East Asian passiflora virus(EAPV),telosma mosaic virus(TelMV)and passion fruit severe mottle virus(PFV),from passionfruit leaves show ing mosaic,chlorosis,and curly-leaf symptoms collected from Yuanmou County,Yunnan Province.Comparison of their coat proteins(CPs)show ed several similar characteristics and some differences.How ever,several basic physicochemical properties and secondary structure of their coat protein and some other properties are similar to each other.These results may provide useful data for the development of antibodies specific to these viruses.展开更多
基金the National Natural Sciences Foundation of China(Nos.81641093,81371790,81371422,81571481 and 81701571)the Fundamental Research Funds for the Central Universities of China and the Translational Medical Research Fund of Wuhan University School of Medicine.
文摘Prototype foamy virus(PFV)is a unique retrovirus that infects animals and humans and does not cause clinical symptoms.Long noncoding RNAs(lncRNAs)are believed to exert multiple regulatory functions during viral infections.Previously,we utilized RNA sequencing(RNA-seq)to characterize and identify the lncRNA lnc-RP5-1086 D14.3.1-1:1(lnc-RP5),which is markedly decreased in PFV-infected cells.However,little is known about the function of lnc-RP5 during PFV infection.In this study,we identified lnc-RP5 as a regulator of the PFV transcriptional transactivator(Tas).Lnc-RP5 enhanced the activity of the PFV internal promoter(IP).The expression of PFV Tas was found to be promoted by lnc-RP5.Moreover,mi R-129-5 p was found to be involved in the lnc-RP5-mediated promotion of PFV IP activity,while the Notch1 protein suppressed the activity of PFV IP and the expression of Tas.Our results demonstrate that lnc-RP5 promotes the expression of PFV Tas through the miR-129-5 p/Notch1/PFV IP axis.This work provides evidence that host lnc RNAs can manipulate PFV replication by employing mi RNAs and proteins during an early viral infection.
文摘电化学作为现代仪器分析重要的组成部分,在生命科学领域正扮演着越来越重要的角色.随着蛋白膜伏安法(protein film voltammetery,PFV)的不断完善,以及表面修饰技术、纳米技术、分子标记与分子识别技术等相关技术的不断发展,在蛋白酶研究领域,电化学正逐渐克服以往手段较为单一、研究目标不够多样、获取信息不够丰富等弊端.以本课题组在该领域所取得的一些研究成果为主,综述蛋白酶电化学研究的现状和发展趋势.
基金National Natural Science Foundation of China(31972235)。
文摘Potyviruses are plant viruses that infect various kinds of economic crops and cause enormous losses.Here w e identified three potyviruses,East Asian passiflora virus(EAPV),telosma mosaic virus(TelMV)and passion fruit severe mottle virus(PFV),from passionfruit leaves show ing mosaic,chlorosis,and curly-leaf symptoms collected from Yuanmou County,Yunnan Province.Comparison of their coat proteins(CPs)show ed several similar characteristics and some differences.How ever,several basic physicochemical properties and secondary structure of their coat protein and some other properties are similar to each other.These results may provide useful data for the development of antibodies specific to these viruses.
