Objective:To observe the change and clinical significance of serum PG in patients with chronic atrophic gastritis (CAG).Methods:ELISA was used to detect the peripheral blood PG level in patients confirmed with CAG, ga...Objective:To observe the change and clinical significance of serum PG in patients with chronic atrophic gastritis (CAG).Methods:ELISA was used to detect the peripheral blood PG level in patients confirmed with CAG, gastric polyps, and gastric cancer who were admitted in our hospital from January, 2015 to January, 2016. The normal individuals who came for physical examinations were served as the control group. The peripheral blood PG level in patients with various gastric diseases was observed.Results: The serum PGⅠ expression and PG I/PGⅡ in the gastritis group were significantly lower than those in the gastric polyps group and control group, but were significantly higher than those in the gastric cancer group;while PGⅡ expression was significantly higher than that in the gastric polyps group and control group, but was significantly lower than those in the gastric cancer group. PGⅠ expression and PG I/ PGⅡ in the gastric polyps group were significantly higher than those in the gastritis group and gastric cancer group, while PGⅡ expression was significantly lower than that in the gastritis group and gastric cancer group. PGⅠ expression and PG I/ PGⅡ in the gastric cancer group were significantly lower than those in the other three groups, while PGⅡ expression was significantly higher than that in the other three groups. The serum PGⅠ expression in patients with positive HP infection in the gastritis group and gastric cancer group was significantly higher than that in patients with negative HP infection, but the comparison of PG I/ PGⅡ was not statistically significant. The serum PGⅠ expression and PG I/ PGⅡ in patients with negative and positive HP infection in the gastritis group were significantly higher than those in patients with negative and positive HP infection in the gastric cancer group;while PGⅡ expression was significantly was significantly lower than that in the gastric cancer group.Conclusions: PGⅠ expression and PG I/ PGⅡ in patients with CAG are significantly reduced, but PGⅡ expression is significantly strengthened;while PGⅠ expression and PG I/ PGⅡ are significantly higher than those in the gastric cancer group, but PGⅡ expression is significantly lower than that in the gastric cancer group. It should pay attention to the changes of the above indicators in the clinical treatment. If PGⅠ expression and PG I/ PGⅡ are significantly reduced, but PGⅡ expression is significantly strengthened, it should pay attention to the probability of condition deterioration.展开更多
Firmness is one of the most important fruit quality traits in strawberries.The postharvest shelf life of this soft fruit is highly limited by the loss of firmness,where cell wall disassembly plays an important role.Pr...Firmness is one of the most important fruit quality traits in strawberries.The postharvest shelf life of this soft fruit is highly limited by the loss of firmness,where cell wall disassembly plays an important role.Previous studies demonstrated that the polygalacturonase FaPG1 has a key role in remodelling pectins during strawberry softening.In this study,FaPG1 knockout strawberry plants have been generated using the CRISPR/Cas9 system delivered via Agrobacterium tumefaciens.Ten independent lines,cv.“Chandler”,were obtained,and all of them were successfully edited as determined by PCR amplification and T7 endonuclease assay.The targeted mutagenesis insertion and deletion rates were analyzed using targeted deep sequencing.The percentage of edited sequences varied from 47%up to almost 100%,being higher than 95%for seven of the selected lines.Phenotypic analyses showed that 7 out of the eight lines analyzed produced fruits significantly firmer than the control,ranging from 33 to 70%increase in firmness.There was a positive relationship between the degree of FaPG1 editing and the rise in fruit firmness.Minor changes were observed in other fruit quality traits,such as colour,soluble solids,titratable acidity or anthocyanin content.Edited fruits showed a reduced softening rate during postharvest,displayed a reduced transpirational water loss,and were less damaged by Botrytis cinerea inoculation.The analysis of four potential off-target sites revealed no mutation events.In conclusion,editing the FaPG1 gene using the CRISPR/Cas9 system is an efficient method for improving strawberry fruit firmness and shelf life.展开更多
High-quality datasets are critical for the development of advanced machine-learning algorithms in seismology.Here,we present an earthquake dataset based on the ChinArray Phase I records(X1).ChinArray Phase I was deplo...High-quality datasets are critical for the development of advanced machine-learning algorithms in seismology.Here,we present an earthquake dataset based on the ChinArray Phase I records(X1).ChinArray Phase I was deployed in the southern north-south seismic zone(20°N-32°N,95°E-110°E)in 2011-2013 using 355 portable broadband seismic stations.CREDIT-X1local,the first release of the ChinArray Reference Earthquake Dataset for Innovative Techniques(CREDIT),includes comprehensive information for the 105,455 local events that occurred in the southern north-south seismic zone during array observation,incorporating them into a single HDF5 file.Original 100-Hz sampled three-component waveforms are organized by event for stations within epicenter distances of 1,000 km,and records of≥200 s are included for each waveform.Two types of phase labels are provided.The first includes manually picked labels for 5,999 events with magnitudes≥2.0,providing 66,507 Pg,42,310 Sg,12,823 Pn,and 546 Sn phases.The second contains automatically labeled phases for 105,442 events with magnitudes of−1.6 to 7.6.These phases were picked using a recurrent neural network phase picker and screened using the corresponding travel time curves,resulting in 1,179,808 Pg,884,281 Sg,176,089 Pn,and 22,986 Sn phases.Additionally,first-motion polarities are included for 31,273 Pg phases.The event and station locations are provided,so that deep learning networks for both conventional phase picking and phase association can be trained and validated.The CREDIT-X1local dataset is the first million-scale dataset constructed from a dense seismic array,which is designed to support various multi-station deep-learning methods,high-precision focal mechanism inversion,and seismic tomography studies.Additionally,owing to the high seismicity in the southern north-south seismic zone in China,this dataset has great potential for future scientific discoveries.展开更多
文摘Objective:To observe the change and clinical significance of serum PG in patients with chronic atrophic gastritis (CAG).Methods:ELISA was used to detect the peripheral blood PG level in patients confirmed with CAG, gastric polyps, and gastric cancer who were admitted in our hospital from January, 2015 to January, 2016. The normal individuals who came for physical examinations were served as the control group. The peripheral blood PG level in patients with various gastric diseases was observed.Results: The serum PGⅠ expression and PG I/PGⅡ in the gastritis group were significantly lower than those in the gastric polyps group and control group, but were significantly higher than those in the gastric cancer group;while PGⅡ expression was significantly higher than that in the gastric polyps group and control group, but was significantly lower than those in the gastric cancer group. PGⅠ expression and PG I/ PGⅡ in the gastric polyps group were significantly higher than those in the gastritis group and gastric cancer group, while PGⅡ expression was significantly lower than that in the gastritis group and gastric cancer group. PGⅠ expression and PG I/ PGⅡ in the gastric cancer group were significantly lower than those in the other three groups, while PGⅡ expression was significantly higher than that in the other three groups. The serum PGⅠ expression in patients with positive HP infection in the gastritis group and gastric cancer group was significantly higher than that in patients with negative HP infection, but the comparison of PG I/ PGⅡ was not statistically significant. The serum PGⅠ expression and PG I/ PGⅡ in patients with negative and positive HP infection in the gastritis group were significantly higher than those in patients with negative and positive HP infection in the gastric cancer group;while PGⅡ expression was significantly was significantly lower than that in the gastric cancer group.Conclusions: PGⅠ expression and PG I/ PGⅡ in patients with CAG are significantly reduced, but PGⅡ expression is significantly strengthened;while PGⅠ expression and PG I/ PGⅡ are significantly higher than those in the gastric cancer group, but PGⅡ expression is significantly lower than that in the gastric cancer group. It should pay attention to the changes of the above indicators in the clinical treatment. If PGⅠ expression and PG I/ PGⅡ are significantly reduced, but PGⅡ expression is significantly strengthened, it should pay attention to the probability of condition deterioration.
基金supported by the Ministerio de Ciencia,Innovación y Universidades and FEDER EU funds(grant numbers AGL2017-86531-C2-1R and PID2020-118468RB-C21)the University of Malaga(grant number B1-2020_09)+1 种基金CS-R was awarded a PhD Fellowship from the Ministerio de Ciencia,Innovación y Universidades(PRE2018-085509)PhD Program Advanced Biotechnology,University of Málaga.
文摘Firmness is one of the most important fruit quality traits in strawberries.The postharvest shelf life of this soft fruit is highly limited by the loss of firmness,where cell wall disassembly plays an important role.Previous studies demonstrated that the polygalacturonase FaPG1 has a key role in remodelling pectins during strawberry softening.In this study,FaPG1 knockout strawberry plants have been generated using the CRISPR/Cas9 system delivered via Agrobacterium tumefaciens.Ten independent lines,cv.“Chandler”,were obtained,and all of them were successfully edited as determined by PCR amplification and T7 endonuclease assay.The targeted mutagenesis insertion and deletion rates were analyzed using targeted deep sequencing.The percentage of edited sequences varied from 47%up to almost 100%,being higher than 95%for seven of the selected lines.Phenotypic analyses showed that 7 out of the eight lines analyzed produced fruits significantly firmer than the control,ranging from 33 to 70%increase in firmness.There was a positive relationship between the degree of FaPG1 editing and the rise in fruit firmness.Minor changes were observed in other fruit quality traits,such as colour,soluble solids,titratable acidity or anthocyanin content.Edited fruits showed a reduced softening rate during postharvest,displayed a reduced transpirational water loss,and were less damaged by Botrytis cinerea inoculation.The analysis of four potential off-target sites revealed no mutation events.In conclusion,editing the FaPG1 gene using the CRISPR/Cas9 system is an efficient method for improving strawberry fruit firmness and shelf life.
基金funded by the National Key R&D Program of China (No. 2021YFC3000702)the Special Fund of the Institute of Geophysics, China Earthquake Administration (No. DQJB20B15)+2 种基金the National Natural Science Foundation of China youth Grant (No. 41804059)the Joint Funds of the National Natural Science Foundation of China (No. U223920029)the Science for Earthquake Resilience of China Earthquake Administration (No. XH211103)
文摘High-quality datasets are critical for the development of advanced machine-learning algorithms in seismology.Here,we present an earthquake dataset based on the ChinArray Phase I records(X1).ChinArray Phase I was deployed in the southern north-south seismic zone(20°N-32°N,95°E-110°E)in 2011-2013 using 355 portable broadband seismic stations.CREDIT-X1local,the first release of the ChinArray Reference Earthquake Dataset for Innovative Techniques(CREDIT),includes comprehensive information for the 105,455 local events that occurred in the southern north-south seismic zone during array observation,incorporating them into a single HDF5 file.Original 100-Hz sampled three-component waveforms are organized by event for stations within epicenter distances of 1,000 km,and records of≥200 s are included for each waveform.Two types of phase labels are provided.The first includes manually picked labels for 5,999 events with magnitudes≥2.0,providing 66,507 Pg,42,310 Sg,12,823 Pn,and 546 Sn phases.The second contains automatically labeled phases for 105,442 events with magnitudes of−1.6 to 7.6.These phases were picked using a recurrent neural network phase picker and screened using the corresponding travel time curves,resulting in 1,179,808 Pg,884,281 Sg,176,089 Pn,and 22,986 Sn phases.Additionally,first-motion polarities are included for 31,273 Pg phases.The event and station locations are provided,so that deep learning networks for both conventional phase picking and phase association can be trained and validated.The CREDIT-X1local dataset is the first million-scale dataset constructed from a dense seismic array,which is designed to support various multi-station deep-learning methods,high-precision focal mechanism inversion,and seismic tomography studies.Additionally,owing to the high seismicity in the southern north-south seismic zone in China,this dataset has great potential for future scientific discoveries.
