To discriminate goatpoxvirus (GPV) and sheeppoxvirus (SPV), the p32 genes and G-protein-coupled chemokine receptor (GpCR) genes amplified from three SPV field isolates and two GPV field isolates were sequenced and com...To discriminate goatpoxvirus (GPV) and sheeppoxvirus (SPV), the p32 genes and G-protein-coupled chemokine receptor (GpCR) genes amplified from three SPV field isolates and two GPV field isolates were sequenced and compared with the corresponding sequences deposited in GenBank. Comparison of Hinf I restriction enzyme sites of p32 genes showed that there were two sites located at 391 and 691 bp, respectively, among all the SPV strains, and one site at 688 bp among GPV strains. Cladogram generated by sequence alignment of GpCR genes showed that the SPV and GPV belonged to two different branches. These results indicate that p32. and GpCR genes can be candidates used for discrimination of GPV and SPV.展开更多
目的:分析不同rag基因型牙龈卟啉单胞菌在青春期牙龈炎中的分布状况。方法:分别选取青春期牙龈炎(GI≥1,PD≥3 mm)64例及牙周健康者(GI=0,PD<3 mm)56例作为研究对象,采用16S r DNA聚合酶链反应(PCR)法检测受试者龈沟液标本中牙龈卟...目的:分析不同rag基因型牙龈卟啉单胞菌在青春期牙龈炎中的分布状况。方法:分别选取青春期牙龈炎(GI≥1,PD≥3 mm)64例及牙周健康者(GI=0,PD<3 mm)56例作为研究对象,采用16S r DNA聚合酶链反应(PCR)法检测受试者龈沟液标本中牙龈卟啉单胞菌及致病岛rag基因,分析其与临床指标之间的相互关系。结果:病例组病变位点和对照组牙龈卟啉单胞菌阳性检出率分别为65.63%(42/64)、33.93%(19/56)(P<0.05)。牙龈卟啉单胞菌阳性患者(n=37)的rag基因型检出率依次为rag-3(16/37)、rag-1(9/37))、rag-4(7/37)和rag-2(5/37)。结论:青春期牙龈炎患者龈沟液中牙龈卟啉单胞菌存在rag基因多态性,其中rag-1和rag-3基因型牙龈卟啉单胞菌与青春期牙龈炎的发生发展关系密切。展开更多
文摘【目的】解析桃果实黏离核性状的分子机制。【方法】以不同黏离核性状的45份桃野生资源、地方品种和育成品种为试材,采用第2代测序技术鉴定2个通过连锁分析得到的、串联重复的endo-PG基因在45份种质中的SNPs(single nucleotide polymorphisms)信息;继而采用GLM(Generalized Linear Models)和MLM(Mixed Linear Models)模型进行关联分析,鉴定与黏离核性状关联的SNPs。【结果】在ppa006839m和ppa006857m这2个endo-PG基因内共鉴定到98个SNPs,平均1 SNPs/59 bp。在少数位点上,离核和黏核种质的SNPs多态性存在差异,但整体多样性水平差异不显著,暗示这2个endo-PG基因倾向于中性进化。根据98个SNPs位点信息构建的系统发育树能够反映这些种质的进化关系,即光核桃、甘肃桃最为原始,山桃其次,普通桃最为进化。GLM关联分析结果表明,在ppa006839m基因的内含子3区存在一个与黏离核性状显著关联的位点,对表型变异的解释率为21.1%,但经Bonferroni多重检验校正后,该位点并无统计学意义。而MLM模型的关联分析并没有检测到显著关联位点。【结论】研究结果虽然没能解释果实黏离核的分子机制,但暗示ppa006839m可能与该性状存在更为紧密的联系。同时,研究结果也为果实黏离核性状的分子标记辅助育种奠定基础。
基金supported by the grants from the Special Fund for Agricultural Biotechnology of Gansu Province (092NKDA032)National Key Technology R&D Program (2006BAD06A11 and 2006BAD06A17)
文摘To discriminate goatpoxvirus (GPV) and sheeppoxvirus (SPV), the p32 genes and G-protein-coupled chemokine receptor (GpCR) genes amplified from three SPV field isolates and two GPV field isolates were sequenced and compared with the corresponding sequences deposited in GenBank. Comparison of Hinf I restriction enzyme sites of p32 genes showed that there were two sites located at 391 and 691 bp, respectively, among all the SPV strains, and one site at 688 bp among GPV strains. Cladogram generated by sequence alignment of GpCR genes showed that the SPV and GPV belonged to two different branches. These results indicate that p32. and GpCR genes can be candidates used for discrimination of GPV and SPV.
文摘目的:分析不同rag基因型牙龈卟啉单胞菌在青春期牙龈炎中的分布状况。方法:分别选取青春期牙龈炎(GI≥1,PD≥3 mm)64例及牙周健康者(GI=0,PD<3 mm)56例作为研究对象,采用16S r DNA聚合酶链反应(PCR)法检测受试者龈沟液标本中牙龈卟啉单胞菌及致病岛rag基因,分析其与临床指标之间的相互关系。结果:病例组病变位点和对照组牙龈卟啉单胞菌阳性检出率分别为65.63%(42/64)、33.93%(19/56)(P<0.05)。牙龈卟啉单胞菌阳性患者(n=37)的rag基因型检出率依次为rag-3(16/37)、rag-1(9/37))、rag-4(7/37)和rag-2(5/37)。结论:青春期牙龈炎患者龈沟液中牙龈卟啉单胞菌存在rag基因多态性,其中rag-1和rag-3基因型牙龈卟啉单胞菌与青春期牙龈炎的发生发展关系密切。
