Objective:To investigate the effect of PGA/PLA scaffolds on tissue engineering cartilage reconstruction in knee osteoarthritis. Methods:Thirty Japanese white rabbits were divided into three groups. The first group was...Objective:To investigate the effect of PGA/PLA scaffolds on tissue engineering cartilage reconstruction in knee osteoarthritis. Methods:Thirty Japanese white rabbits were divided into three groups. The first group was healthy (group H):normal Japanese white rabbits, without knee osteoarthritis;the second group, knee osteoarthritis group ( Group k):Normal Japanese white rabbits were diagnosed with knee osteoarthritis by model preparation;Group 3, tissue engineering group (Group T):Tissue engineering cartilage reconstruction of Japanese knee white rabbits with knee osteoarthritis. 10 white rabbits per group. The cartilage histological score, HE staining, immunohistochemistry, Western blot, qRT-PCR analysis of H group, k group, T group cartilage histological score, cartilage histopathology and morphological changes, cartilage tissue The difference in Col-Ⅱ protein content and Col-Ⅱ mRNA content was used to investigate the effect of PGA/PLA scaffold material on tissue engineering cartilage reconstruction of knee osteoarthritis. Results:Cartilage tissue was scored according to international histological scoring criteria. The cartilage of the k group was severely fibrotic, the surface of the joint was irregular, and there were many fluids in the cavity, and the defect was severe. In the T group, the fibrosis phenomenon was alleviated, the surface was regular, the area of the effusion in the cavity was reduced, no depression occurred, and the surface of the joint was regular. Arthritis symptoms and cartilage tissue scores were significantly improved in group T and group k (P<0.05). The chondrocytes in the H group were densely distributed, and the k group was disorderly and sparse. In the H group, the cartilage layer of the knee joint was thick, and the cartilage layer in the k group was thin and damaged. The chondrocytes in the H group were located in the lacuna in the stroma, and there were cartilage sacs. The k group had obvious defects and no cartilage capsule structure (all P<0.05). Compared with the k group, the cartilage layer at the knee joint was thicker and the cells were densely distributed. The cartilage sac of some cells could be seen (P<0.05). There were more stromal collagen positive cells in the knee joint of group H. The number of cells in the k group was the least, the cartilage matrix was the most severely damaged, and the chondrocytes and the bone matrix were loose. Compared with group T and group k, the damage of positive cartilage matrix was reduced, and the number of cells was significantly increased (P<0.05). Result:There was a significant difference between the k group and the T group (P<0.05). Western blot was used to immunoblot the content of Col-Ⅱ protein in cartilage tissues of group H, k and T. According to the gray scale analysis, the content of Col-Ⅱ protein was the highest in group H, and the lowest in group k, group T and k. Compared with the group, the content of Col-Ⅱ protein was significantly increased (P<0.05). The expression of Col-Ⅱ mRNA in cartilage tissue of H group was the highest in group H, and the expression of Col-Ⅱ mRNA was the lowest in group k. The expression of Col-Ⅱ mRNA was significantly increased in group T and group k (all P< 0.05). Conclusions:PGA/PLA was used as scaffold material to reconstruct knee osteochondral tissue by tissue engineering method, which has obvious therapeutic effect on knee osteoarthritis.展开更多
The homopolymerization and copolymerization of glycolide (GA) and lactide (LA) are des-cribed. The resulting polymers are characterized by differential scanning calorimetry (DSC),thermogravimetric analysis (TGA), 300 ...The homopolymerization and copolymerization of glycolide (GA) and lactide (LA) are des-cribed. The resulting polymers are characterized by differential scanning calorimetry (DSC),thermogravimetric analysis (TGA), 300 MHz proton nuclear magnetic resonance spectroscopy(NMR), infrared spectroscopy, and X-ray diffraction. The rheological properties of PGA andPGLA 910 are evaluated by shear experiments. The biodegradation has been studied in vivo. Theresults show that the polyesters can be absorbed by the muscles of rabbits after 60 days.展开更多
Four kinds of braided yarns were produced with different proportions of polyglycolic acid( PGA) and polylactic acid( PLA)multifilaments( 2PGA /1PLA, 2PGA /2PLA, 3PGA /1PLA, and3PGA /2PLA). A novel artificial plain sti...Four kinds of braided yarns were produced with different proportions of polyglycolic acid( PGA) and polylactic acid( PLA)multifilaments( 2PGA /1PLA, 2PGA /2PLA, 3PGA /1PLA, and3PGA /2PLA). A novel artificial plain stitch scaffold reinforcement was manufactured by braiding technology and knitting technology respectively. Tendon scaffold reinforcements were investigated for 8weeks in phosphate buffered solution( PBS)( pH = 7. 4) at 37 ℃.The degradation was studied with regard to the mass loss,tensile properties,grams per square meter( g /m2),thickness,caliber,and porosity of scaffold reinforcements. The experimental showed that during the process of 8-week degradation,the mass losses of scaffold reinforcements were small in the first 3-week,but they increased rapidly after 3-week,and the speeds tended to be small gradually after 6-week; the tensile properties dropped rapidly in the first 2-week; the grams per square meter and thicknesses speeded down obviously between 3-week to 6-week. The caliber and porosity of scaffold reinforcements first decreased and then increased gradually. The porosity can reach more than 97%.展开更多
文摘Objective:To investigate the effect of PGA/PLA scaffolds on tissue engineering cartilage reconstruction in knee osteoarthritis. Methods:Thirty Japanese white rabbits were divided into three groups. The first group was healthy (group H):normal Japanese white rabbits, without knee osteoarthritis;the second group, knee osteoarthritis group ( Group k):Normal Japanese white rabbits were diagnosed with knee osteoarthritis by model preparation;Group 3, tissue engineering group (Group T):Tissue engineering cartilage reconstruction of Japanese knee white rabbits with knee osteoarthritis. 10 white rabbits per group. The cartilage histological score, HE staining, immunohistochemistry, Western blot, qRT-PCR analysis of H group, k group, T group cartilage histological score, cartilage histopathology and morphological changes, cartilage tissue The difference in Col-Ⅱ protein content and Col-Ⅱ mRNA content was used to investigate the effect of PGA/PLA scaffold material on tissue engineering cartilage reconstruction of knee osteoarthritis. Results:Cartilage tissue was scored according to international histological scoring criteria. The cartilage of the k group was severely fibrotic, the surface of the joint was irregular, and there were many fluids in the cavity, and the defect was severe. In the T group, the fibrosis phenomenon was alleviated, the surface was regular, the area of the effusion in the cavity was reduced, no depression occurred, and the surface of the joint was regular. Arthritis symptoms and cartilage tissue scores were significantly improved in group T and group k (P<0.05). The chondrocytes in the H group were densely distributed, and the k group was disorderly and sparse. In the H group, the cartilage layer of the knee joint was thick, and the cartilage layer in the k group was thin and damaged. The chondrocytes in the H group were located in the lacuna in the stroma, and there were cartilage sacs. The k group had obvious defects and no cartilage capsule structure (all P<0.05). Compared with the k group, the cartilage layer at the knee joint was thicker and the cells were densely distributed. The cartilage sac of some cells could be seen (P<0.05). There were more stromal collagen positive cells in the knee joint of group H. The number of cells in the k group was the least, the cartilage matrix was the most severely damaged, and the chondrocytes and the bone matrix were loose. Compared with group T and group k, the damage of positive cartilage matrix was reduced, and the number of cells was significantly increased (P<0.05). Result:There was a significant difference between the k group and the T group (P<0.05). Western blot was used to immunoblot the content of Col-Ⅱ protein in cartilage tissues of group H, k and T. According to the gray scale analysis, the content of Col-Ⅱ protein was the highest in group H, and the lowest in group k, group T and k. Compared with the group, the content of Col-Ⅱ protein was significantly increased (P<0.05). The expression of Col-Ⅱ mRNA in cartilage tissue of H group was the highest in group H, and the expression of Col-Ⅱ mRNA was the lowest in group k. The expression of Col-Ⅱ mRNA was significantly increased in group T and group k (all P< 0.05). Conclusions:PGA/PLA was used as scaffold material to reconstruct knee osteochondral tissue by tissue engineering method, which has obvious therapeutic effect on knee osteoarthritis.
文摘The homopolymerization and copolymerization of glycolide (GA) and lactide (LA) are des-cribed. The resulting polymers are characterized by differential scanning calorimetry (DSC),thermogravimetric analysis (TGA), 300 MHz proton nuclear magnetic resonance spectroscopy(NMR), infrared spectroscopy, and X-ray diffraction. The rheological properties of PGA andPGLA 910 are evaluated by shear experiments. The biodegradation has been studied in vivo. Theresults show that the polyesters can be absorbed by the muscles of rabbits after 60 days.
基金Hi-Tech Research and Development Program of China(863 program)(No.2012AA020507)
文摘Four kinds of braided yarns were produced with different proportions of polyglycolic acid( PGA) and polylactic acid( PLA)multifilaments( 2PGA /1PLA, 2PGA /2PLA, 3PGA /1PLA, and3PGA /2PLA). A novel artificial plain stitch scaffold reinforcement was manufactured by braiding technology and knitting technology respectively. Tendon scaffold reinforcements were investigated for 8weeks in phosphate buffered solution( PBS)( pH = 7. 4) at 37 ℃.The degradation was studied with regard to the mass loss,tensile properties,grams per square meter( g /m2),thickness,caliber,and porosity of scaffold reinforcements. The experimental showed that during the process of 8-week degradation,the mass losses of scaffold reinforcements were small in the first 3-week,but they increased rapidly after 3-week,and the speeds tended to be small gradually after 6-week; the tensile properties dropped rapidly in the first 2-week; the grams per square meter and thicknesses speeded down obviously between 3-week to 6-week. The caliber and porosity of scaffold reinforcements first decreased and then increased gradually. The porosity can reach more than 97%.
