Objective:To investigate the effect of Delisheng Injection（得力生注射液 DLS）,a Chinese medicinal compound,DLS combined with cis-platinum（DDP）,an active agent used in lung cancer chemotherapy,on a human highly meta...Objective:To investigate the effect of Delisheng Injection（得力生注射液 DLS）,a Chinese medicinal compound,DLS combined with cis-platinum（DDP）,an active agent used in lung cancer chemotherapy,on a human highly metastatic giant lung carcinoma cell line PGCL3.Methods:The suspended PGCL3 cells at10;/mL cultured in 96-well tissue culture plates were divided into 4 groups:DLS treatment group（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）,DDP treatment group（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL）,combined DLS with DDP treatment group（DLS:DDP 2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL）and a control group.The cytotoxicity of DLS with different concentrations（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）on PGCL3 cells was determined by 3-（4,5）-dimethylthiahiazo（-z-y1）-3,5-di-phenytetrazoliumromide（MTT） assay.Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay.Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells.Results:Compared with the control group,DLS（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL） could significantly decrease cell proliferation,adhesion,invasion and migration abilities（P<0.05）.Cell adhesion,invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP（2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL） compared with DDP single-agent treatment（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL,P<0.05）,respectively.Conclusions:DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis.Our research provided a experimental basis about the treatment on highly metastatic lung caner.展开更多
基金Supported by the Project of Science and Technology Development in Shaanxi Province of China(No.2008KG10-01)
文摘Objective:To investigate the effect of Delisheng Injection（得力生注射液 DLS）,a Chinese medicinal compound,DLS combined with cis-platinum（DDP）,an active agent used in lung cancer chemotherapy,on a human highly metastatic giant lung carcinoma cell line PGCL3.Methods:The suspended PGCL3 cells at10;/mL cultured in 96-well tissue culture plates were divided into 4 groups:DLS treatment group（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）,DDP treatment group（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL）,combined DLS with DDP treatment group（DLS:DDP 2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL）and a control group.The cytotoxicity of DLS with different concentrations（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL）on PGCL3 cells was determined by 3-（4,5）-dimethylthiahiazo（-z-y1）-3,5-di-phenytetrazoliumromide（MTT） assay.Effect of DLS on adhesion of PGCL-3 cells was tested by cell-matrigel adhesion assay.Chemotactic movement model of transwell camerula was used to determine the effect of DLS on invasion and migration of PGCL-3 cells.Results:Compared with the control group,DLS（2 μL/mL,5 μL/mL,10 μL/mL,25 μL/mL） could significantly decrease cell proliferation,adhesion,invasion and migration abilities（P<0.05）.Cell adhesion,invasion and migration abilities were significantly decreased after combination treatment of DLS:DDP（2 μL/mL:1 μg/mL,5 μL/mL:2 μg/mL,10 μL/mL:5 μg/mL,25 μL/mL:15 μg/mL） compared with DDP single-agent treatment（1 μg/mL,2 μg/mL,5 μg/mL,15 μg/mL,P<0.05）,respectively.Conclusions:DLS single-agent has a satisfying inhibition effect in PGCL3 cell line and DLS might enhance the inhibition effect of DDP on cancer metastasis.Our research provided a experimental basis about the treatment on highly metastatic lung caner.
