The serine/threonine protein kinase B(PKB) is related to cellular survival and regulation. PKB is composed of PH domain, catalytic domain and carboxyl terminal regulator domain. The PH domain of PKB is crucial to the ...The serine/threonine protein kinase B(PKB) is related to cellular survival and regulation. PKB is composed of PH domain, catalytic domain and carboxyl terminal regulator domain. The PH domain of PKB is crucial to the activation of kinase. In order to investigate the function and the structure function relationship of PKB, the cDNA coding fragment of PKB PH domain was amplified from human dental pulp mRNA by RT PCR and cloned into pMD18 T vector to analyze the sequence. The result showed that DNA sequence of cloned human PKB PH domain was consistent with that reported previously. To express PKB PH domain, the cDNA was subcloned into expression vector pRSET A which was then transformed into E.coli BL21(DE3) pLysS, and the strain highly expressing soluble 6His PKB PH domain in minimal medium was obtained. The fusion protein was purified by Ni 2+ NTA agarose beads. The secondary structure of the purified 6His PKB PH domain fusion protein was analysed by circular dichroism. The results indicated that the PH domain was composed of α helix 1 7%,β pleated sheet 80 5% and radom coli 17 8%.展开更多
Bi4Ti3O12 （BIT） crystals were controllably synthesized via a facile hydrothermal process without adding any surfactant or template. The morphologies of BIT with nanosphere, nanoplate, nanobelt, and nanosheet can be ...Bi4Ti3O12 （BIT） crystals were controllably synthesized via a facile hydrothermal process without adding any surfactant or template. The morphologies of BIT with nanosphere, nanoplate, nanobelt, and nanosheet can be selectively obtained by adjusting the pH value of the reactant. The formation mechanisms of these distinctive morphologies were then discussed based on the structural analysis of samples obtained at different pH values. BIT sample prepared at pH=1 showed the highest photocatalytic activity under visible light irradiation. The photocatalytic activities difference for the BIT samples synthesized at different pH values was studied based on their shape, size, and the variation of local structure.展开更多
The supply of cadmium from soil to plant roots mainly depends on the diffusion process. This work was conducted tostudy the effect of some soil properties on cadmium diffusion coefficient (D) in soil. Measurements we...The supply of cadmium from soil to plant roots mainly depends on the diffusion process. This work was conducted tostudy the effect of some soil properties on cadmium diffusion coefficient (D) in soil. Measurements were made using the Shofield and Graham-Bryce's isotopic labelling method. Cadmium diffusion coefficients varied from 10￣(-7) to 10￣(-9) cm￣2s￣(-1),Higher values were observed in acid sandy soils and lower values in calcareous clay soils. Liming an acid soil resulted in a sub-stantial decrease of D. Addition of cadmium as nitrate salt generally increased D, while addition of sewage sludge and organ-ic matter resulted in a significant decrease of cadmium diffusion. The rhizospheric activity also induced a moderate reduction in D. The relationships between D 10￣(-9)cm￣2s￣(-1)) on the one hand and soil PH, moisture (Mc, g kg￣(-1)), organic matter (OM, gkg￣1 ), clay (Cy, g kg￣(-1)) and cadmium content (Cd, mg kg￣(-1)) on the other were obtained by the multigle regression:D=182. 1-29.g1 pH+0.210Mc-0.303OM+0.011Cy+1.64Cd (R￣2=0.859,n=22 ).展开更多
文摘The serine/threonine protein kinase B(PKB) is related to cellular survival and regulation. PKB is composed of PH domain, catalytic domain and carboxyl terminal regulator domain. The PH domain of PKB is crucial to the activation of kinase. In order to investigate the function and the structure function relationship of PKB, the cDNA coding fragment of PKB PH domain was amplified from human dental pulp mRNA by RT PCR and cloned into pMD18 T vector to analyze the sequence. The result showed that DNA sequence of cloned human PKB PH domain was consistent with that reported previously. To express PKB PH domain, the cDNA was subcloned into expression vector pRSET A which was then transformed into E.coli BL21(DE3) pLysS, and the strain highly expressing soluble 6His PKB PH domain in minimal medium was obtained. The fusion protein was purified by Ni 2+ NTA agarose beads. The secondary structure of the purified 6His PKB PH domain fusion protein was analysed by circular dichroism. The results indicated that the PH domain was composed of α helix 1 7%,β pleated sheet 80 5% and radom coli 17 8%.
基金ACKNOWLEDGMENTS This work was supported by the National Natural Science Foundation of China （No.61308095）, China Postdoctoral Science Foundation （No.2013M531286）, the Key Laboratory of Preparation and Application Environmentally Friendly Materials of the Ministry of Education of China, and the Science Development Project of Jilin Province No.20130102004JC）. （No.20130522071JH and
文摘Bi4Ti3O12 （BIT） crystals were controllably synthesized via a facile hydrothermal process without adding any surfactant or template. The morphologies of BIT with nanosphere, nanoplate, nanobelt, and nanosheet can be selectively obtained by adjusting the pH value of the reactant. The formation mechanisms of these distinctive morphologies were then discussed based on the structural analysis of samples obtained at different pH values. BIT sample prepared at pH=1 showed the highest photocatalytic activity under visible light irradiation. The photocatalytic activities difference for the BIT samples synthesized at different pH values was studied based on their shape, size, and the variation of local structure.
文摘The supply of cadmium from soil to plant roots mainly depends on the diffusion process. This work was conducted tostudy the effect of some soil properties on cadmium diffusion coefficient (D) in soil. Measurements were made using the Shofield and Graham-Bryce's isotopic labelling method. Cadmium diffusion coefficients varied from 10￣(-7) to 10￣(-9) cm￣2s￣(-1),Higher values were observed in acid sandy soils and lower values in calcareous clay soils. Liming an acid soil resulted in a sub-stantial decrease of D. Addition of cadmium as nitrate salt generally increased D, while addition of sewage sludge and organ-ic matter resulted in a significant decrease of cadmium diffusion. The rhizospheric activity also induced a moderate reduction in D. The relationships between D 10￣(-9)cm￣2s￣(-1)) on the one hand and soil PH, moisture (Mc, g kg￣(-1)), organic matter (OM, gkg￣1 ), clay (Cy, g kg￣(-1)) and cadmium content (Cd, mg kg￣(-1)) on the other were obtained by the multigle regression:D=182. 1-29.g1 pH+0.210Mc-0.303OM+0.011Cy+1.64Cd (R￣2=0.859,n=22 ).
