DEFECTIVE LYMPHOCYTES SUPPORTING HEMATOPOIESIS IN PATIENTS WITH PAROXYSMAL NOCTURNAL HEMOGLOBINURIA

Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired hemo-lytic disease in which there is a stem cell disorder of clonal nature. Previous studies have demonstrated that the numbers of burst-forming units-erythroid (BFU-E) and colony-forming units-granulocyte / macrophage (CFU-GM) from bone marrow of PNH patients growing in the medium containing PHA-LCM from the normai donors were more reduced than those of normai bone marrow. The purpose of present study was to investigate if PNH lymphocytes are defective in supporting hematopoiesis in vitro. PHA-LCM from PNH blood was added to the culture medium for the growth of PNH and normai BFU-E and CFU-GM. The numbers of PNH bone marrow BFU-E and CFU-GM in the medium containing PHA-LCM from PNH blood were less than those from normai blood; the numbers of normai bone marrow BFU-E and CFU-GM grown in the medium containing PHA-LCM from PNH blood were more decreased than those from normai blood. The results suggest that diminished numbers of PNH bone marrovv BFU-

急性非淋巴细胞白血病祖细胞的培养及分析

本文根据Moriyama建立的PHA—LCM液相—半固相二步法培养体系,观察了急性非淋巴细胞白血病(AML)祖细胞(AML-CFU)的生长,并通过比较AML-CFU对PHA-LCM的依赖性,分析了AML-CFU的异质性。

PHA—LCM与HM—CM对急性髓细胞白血病集落生长的研究

急性髓细胞白血病(AML)祖细胞体外培养应用Pike和Robinson的双层琼脂培养法集落产率很低,多数为细胞簇,已见于Moore等的报道、1976年Dicke等应用植物血凝素—白细胞条件培养基(PHA—LCM),结果形成大量的白血病祖细胞集落(CFU—AML)。近来国内一些报道应用胎肌一条件培养基(HM—CM)作为集落刺激因子(CSF)